HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice
Bone marrow-derived mesenchymal stem cells (BMSCs) can differentiate into hepatocyte-like cells (HLCs) to alleviate acute liver injury (ALI). Herpetfluorenone (HPF), as an active ingredient in the dried, mature seeds <i>Herpetospermum caudigerum</i> Wall, used in Tibetan medicine, has be...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5686 |
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| author | Bin Yang Qiu-Lin Luo Nan Wang Yan-Ting Hu Wen-Xin Zheng Hong Li Maimaitituxun Maierziya Jian Gu Qin Wang |
| author_facet | Bin Yang Qiu-Lin Luo Nan Wang Yan-Ting Hu Wen-Xin Zheng Hong Li Maimaitituxun Maierziya Jian Gu Qin Wang |
| author_sort | Bin Yang |
| collection | DOAJ |
| description | Bone marrow-derived mesenchymal stem cells (BMSCs) can differentiate into hepatocyte-like cells (HLCs) to alleviate acute liver injury (ALI). Herpetfluorenone (HPF), as an active ingredient in the dried, mature seeds <i>Herpetospermum caudigerum</i> Wall, used in Tibetan medicine, has been proven to effectively alleviate ALI. Therefore, the purpose of this study was to determine whether HPF can promote the differentiation of BMSCs into HLCs and promote ALI recovery. Mouse BMSCs were isolated, and the BMSCs’ differentiation into HLCs was induced by HPF and hepatocyte growth factor (HGF). Under the induction of HPF and HGF, the expression of hepatocellular specific markers and the accumulation of glycogen and lipids in the BMSCs increased, indicating that BMSCs successfully differentiated into HLCs. Then, the ALI mouse model was established, using carbon tetrachloride, followed by an intravenous injection of BMSCs. Then, only HPF was injected intraperitoneally, in order to verify the effect of HPF in vivo. In vivo imaging was used to detect the homing ability of HPF–BMSCs, and it was detected that HPF–BMSCs significantly increased the levels of serum AST, ALT and ALP in the liver of ALI mice, and alleviated liver cell necrosis, oxidative stress and liver pathology. In conclusion, HPF can promote the differentiation of BMSCs into HLCs and promote the recovery of ALI in mice. |
| first_indexed | 2024-03-11T06:25:28Z |
| format | Article |
| id | doaj.art-d32657768c4f4d18910c45d4135de516 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:25:28Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d32657768c4f4d18910c45d4135de5162023-11-17T11:37:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246568610.3390/ijms24065686HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in MiceBin Yang0Qiu-Lin Luo1Nan Wang2Yan-Ting Hu3Wen-Xin Zheng4Hong Li5Maimaitituxun Maierziya6Jian Gu7Qin Wang8School of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaSchool of Pharmacy, Southwest Minzu University, Chengdu 610225, ChinaBone marrow-derived mesenchymal stem cells (BMSCs) can differentiate into hepatocyte-like cells (HLCs) to alleviate acute liver injury (ALI). Herpetfluorenone (HPF), as an active ingredient in the dried, mature seeds <i>Herpetospermum caudigerum</i> Wall, used in Tibetan medicine, has been proven to effectively alleviate ALI. Therefore, the purpose of this study was to determine whether HPF can promote the differentiation of BMSCs into HLCs and promote ALI recovery. Mouse BMSCs were isolated, and the BMSCs’ differentiation into HLCs was induced by HPF and hepatocyte growth factor (HGF). Under the induction of HPF and HGF, the expression of hepatocellular specific markers and the accumulation of glycogen and lipids in the BMSCs increased, indicating that BMSCs successfully differentiated into HLCs. Then, the ALI mouse model was established, using carbon tetrachloride, followed by an intravenous injection of BMSCs. Then, only HPF was injected intraperitoneally, in order to verify the effect of HPF in vivo. In vivo imaging was used to detect the homing ability of HPF–BMSCs, and it was detected that HPF–BMSCs significantly increased the levels of serum AST, ALT and ALP in the liver of ALI mice, and alleviated liver cell necrosis, oxidative stress and liver pathology. In conclusion, HPF can promote the differentiation of BMSCs into HLCs and promote the recovery of ALI in mice.https://www.mdpi.com/1422-0067/24/6/5686herpetfluorenonebone marrow-derived mesenchymal stem cellshepatocyte-like cellsalleviate acute liver injury |
| spellingShingle | Bin Yang Qiu-Lin Luo Nan Wang Yan-Ting Hu Wen-Xin Zheng Hong Li Maimaitituxun Maierziya Jian Gu Qin Wang HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice International Journal of Molecular Sciences herpetfluorenone bone marrow-derived mesenchymal stem cells hepatocyte-like cells alleviate acute liver injury |
| title | HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice |
| title_full | HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice |
| title_fullStr | HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice |
| title_full_unstemmed | HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice |
| title_short | HPF Modulates the Differentiation of BMSCs into HLCs and Promotes the Recovery of Acute Liver Injury in Mice |
| title_sort | hpf modulates the differentiation of bmscs into hlcs and promotes the recovery of acute liver injury in mice |
| topic | herpetfluorenone bone marrow-derived mesenchymal stem cells hepatocyte-like cells alleviate acute liver injury |
| url | https://www.mdpi.com/1422-0067/24/6/5686 |
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