Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation
The anisotropic microstructure of bone tissue is crucial for appropriate mechanical and biological functions of bone. We recently revealed that the construction of oriented bone matrix is established by osteoblast alignment; there is a quite unique correlation between cell alignment and cell-produce...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-06-01
|
| Series: | Crystals |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2073-4352/6/6/73 |
| _version_ | 1811304567745282048 |
|---|---|
| author | Aira Matsugaki Takayoshi Nakano |
| author_facet | Aira Matsugaki Takayoshi Nakano |
| author_sort | Aira Matsugaki |
| collection | DOAJ |
| description | The anisotropic microstructure of bone tissue is crucial for appropriate mechanical and biological functions of bone. We recently revealed that the construction of oriented bone matrix is established by osteoblast alignment; there is a quite unique correlation between cell alignment and cell-produced bone matrix orientation governed by the molecular interactions between material surface and cells. Titanium and its alloys are one of the most attractive materials for biomedical applications. We previously succeeded in controlling cellular arrangement using the dislocations of a crystallographic slip system in titanium single crystals with hexagonal close-packing (hcp) crystal lattice. Here, we induced a specific surface topography by deformation twinning and dislocation motion to control cell orientation. Dislocation and deformation twinning were introduced into α-titanium polycrystals in compression, inducing a characteristic surface structure involving nanometer-scale highly concentrated twinning traces. The plastic deformation-induced surface topography strongly influenced osteoblast orientation, causing them to align preferentially along the slip and twinning traces. This surface morphology, exhibiting a characteristic grating structure, controlled the localization of focal adhesions and subsequent elongation of stress fibers in osteoblasts. These results indicate that cellular responses against dislocation and deformation twinning are useful for controlling osteoblast alignment and the resulting bone matrix anisotropy. |
| first_indexed | 2024-04-13T08:09:30Z |
| format | Article |
| id | doaj.art-d32983d778764b8ebf61d4a99bcb709e |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-04-13T08:09:30Z |
| publishDate | 2016-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-d32983d778764b8ebf61d4a99bcb709e2022-12-22T02:55:03ZengMDPI AGCrystals2073-43522016-06-01667310.3390/cryst6060073cryst6060073Control of Cellular Arrangement by Surface Topography Induced by Plastic DeformationAira Matsugaki0Takayoshi Nakano1Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, JapanDivision of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, JapanThe anisotropic microstructure of bone tissue is crucial for appropriate mechanical and biological functions of bone. We recently revealed that the construction of oriented bone matrix is established by osteoblast alignment; there is a quite unique correlation between cell alignment and cell-produced bone matrix orientation governed by the molecular interactions between material surface and cells. Titanium and its alloys are one of the most attractive materials for biomedical applications. We previously succeeded in controlling cellular arrangement using the dislocations of a crystallographic slip system in titanium single crystals with hexagonal close-packing (hcp) crystal lattice. Here, we induced a specific surface topography by deformation twinning and dislocation motion to control cell orientation. Dislocation and deformation twinning were introduced into α-titanium polycrystals in compression, inducing a characteristic surface structure involving nanometer-scale highly concentrated twinning traces. The plastic deformation-induced surface topography strongly influenced osteoblast orientation, causing them to align preferentially along the slip and twinning traces. This surface morphology, exhibiting a characteristic grating structure, controlled the localization of focal adhesions and subsequent elongation of stress fibers in osteoblasts. These results indicate that cellular responses against dislocation and deformation twinning are useful for controlling osteoblast alignment and the resulting bone matrix anisotropy.http://www.mdpi.com/2073-4352/6/6/73bone tissue anisotropyosteoblastcell arrangementplastic deformationdislocation glideslip tracedeformation twinning |
| spellingShingle | Aira Matsugaki Takayoshi Nakano Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation Crystals bone tissue anisotropy osteoblast cell arrangement plastic deformation dislocation glide slip trace deformation twinning |
| title | Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation |
| title_full | Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation |
| title_fullStr | Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation |
| title_full_unstemmed | Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation |
| title_short | Control of Cellular Arrangement by Surface Topography Induced by Plastic Deformation |
| title_sort | control of cellular arrangement by surface topography induced by plastic deformation |
| topic | bone tissue anisotropy osteoblast cell arrangement plastic deformation dislocation glide slip trace deformation twinning |
| url | http://www.mdpi.com/2073-4352/6/6/73 |
| work_keys_str_mv | AT airamatsugaki controlofcellulararrangementbysurfacetopographyinducedbyplasticdeformation AT takayoshinakano controlofcellulararrangementbysurfacetopographyinducedbyplasticdeformation |