Cross validation of cut–off limit estimations for milk indicators of subclinical ketosis

Real time analyses of main milk components are attended in milking parlours today. Breeders can know milk composition regularly. Energy (ketosis) milk quotients such as fat/crude protein (F/CP) and fat/lactose (F/L) could be essential for ketosis prevention and losses which can be linked with it. Ai...

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Main Authors: Oto Hanuš, Petr Roubal, Eva Samková, Daniel Falta, Gustav Chládek, Marcela Klimešová Vyletělová
Format: Article
Language:English
Published: Mendel University Press 2013-01-01
Series:Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
Subjects:
Online Access:https://acta.mendelu.cz/61/6/1661/
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author Oto Hanuš
Petr Roubal
Eva Samková
Daniel Falta
Gustav Chládek
Marcela Klimešová Vyletělová
author_facet Oto Hanuš
Petr Roubal
Eva Samková
Daniel Falta
Gustav Chládek
Marcela Klimešová Vyletělová
author_sort Oto Hanuš
collection DOAJ
description Real time analyses of main milk components are attended in milking parlours today. Breeders can know milk composition regularly. Energy (ketosis) milk quotients such as fat/crude protein (F/CP) and fat/lactose (F/L) could be essential for ketosis prevention and losses which can be linked with it. Aim was to do a reciprocal validation of reliability of milk indicators for subclinical ketosis (SK) identification. There were: dairy cows on 1st and other lactations free of clinical and superior subclinical mastitis; 3 herds of Czech Fleckvieh (CF) breed, 3 herds of Holstein (H) breed and 1 herd with CF and H; 329 individual milk samples from summer and winter season and 1st third of lactation. Average milk yield varied from 5,500 to 10,000 kg per lactation. Thresholds of F/CP and F/L were estimated (P < 0.05) according to SK milk acetone cut–off limit (≥10 mg.l−1). These were: for first lactation 1.27 (CF) and 1.32 (H), for other lactations 1.52 (CF) and 1.42 (H) in F/CP; for first lactation 0.84 (CF) and 0.84 (H), for other lactations 0.87 (CF) and 0.85 (H) in F/L. They were used for cross validation (P < 0.001) in the same order: 1.251, 1.31, 1.31 and 1.383 in F/CP; 0.831, 0.821, 0.989 and 0.852 in F/L. Validated cut–off limits are quite similar to their original values. It confirmes good reliability of original limits. The validated cut–off limits of milk indicators (F/CP and F/L) for SK in early lactation can be used at technological innovation in animal husbandry. Use of both quotients could improve the regular investigation of SK in practice.
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spelling doaj.art-d32b7ed0870141c491f48b9ef1bba0e62022-12-22T01:37:28ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102013-01-016161661166710.11118/actaun201361061661Cross validation of cut–off limit estimations for milk indicators of subclinical ketosisOto Hanuš0Petr Roubal1Eva Samková2Daniel Falta3Gustav Chládek4Marcela Klimešová Vyletělová5Dairy Research Institute, Ltd., Prague, Ke Dvoru 12a, 160 00 Praha 6 - Vokovice, Czech RepublicDairy Research Institute, Ltd., Prague, Ke Dvoru 12a, 160 00 Praha 6 - Vokovice, Czech RepublicFaculty of Agriculture, University of South Bohemia České Budějovice, Branišovská 1, 370 05 České Budějovice, Czech RepublicDepartment of Animal Breeding, Mendel University in Brno, 613 00 Brno, Zemědělská 1, Czech RepublicDepartment of Animal Breeding, Mendel University in Brno, 613 00 Brno, Zemědělská 1, Czech RepublicDairy Research Institute, Ltd., Prague, Ke Dvoru 12a, 160 00 Praha 6 - Vokovice, Czech RepublicReal time analyses of main milk components are attended in milking parlours today. Breeders can know milk composition regularly. Energy (ketosis) milk quotients such as fat/crude protein (F/CP) and fat/lactose (F/L) could be essential for ketosis prevention and losses which can be linked with it. Aim was to do a reciprocal validation of reliability of milk indicators for subclinical ketosis (SK) identification. There were: dairy cows on 1st and other lactations free of clinical and superior subclinical mastitis; 3 herds of Czech Fleckvieh (CF) breed, 3 herds of Holstein (H) breed and 1 herd with CF and H; 329 individual milk samples from summer and winter season and 1st third of lactation. Average milk yield varied from 5,500 to 10,000 kg per lactation. Thresholds of F/CP and F/L were estimated (P < 0.05) according to SK milk acetone cut–off limit (≥10 mg.l−1). These were: for first lactation 1.27 (CF) and 1.32 (H), for other lactations 1.52 (CF) and 1.42 (H) in F/CP; for first lactation 0.84 (CF) and 0.84 (H), for other lactations 0.87 (CF) and 0.85 (H) in F/L. They were used for cross validation (P < 0.001) in the same order: 1.251, 1.31, 1.31 and 1.383 in F/CP; 0.831, 0.821, 0.989 and 0.852 in F/L. Validated cut–off limits are quite similar to their original values. It confirmes good reliability of original limits. The validated cut–off limits of milk indicators (F/CP and F/L) for SK in early lactation can be used at technological innovation in animal husbandry. Use of both quotients could improve the regular investigation of SK in practice.https://acta.mendelu.cz/61/6/1661/dairy cowindividual milk samplemilk compositionenergy quotientsubclinical ketosis identification
spellingShingle Oto Hanuš
Petr Roubal
Eva Samková
Daniel Falta
Gustav Chládek
Marcela Klimešová Vyletělová
Cross validation of cut–off limit estimations for milk indicators of subclinical ketosis
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
dairy cow
individual milk sample
milk composition
energy quotient
subclinical ketosis identification
title Cross validation of cut–off limit estimations for milk indicators of subclinical ketosis
title_full Cross validation of cut–off limit estimations for milk indicators of subclinical ketosis
title_fullStr Cross validation of cut–off limit estimations for milk indicators of subclinical ketosis
title_full_unstemmed Cross validation of cut–off limit estimations for milk indicators of subclinical ketosis
title_short Cross validation of cut–off limit estimations for milk indicators of subclinical ketosis
title_sort cross validation of cut off limit estimations for milk indicators of subclinical ketosis
topic dairy cow
individual milk sample
milk composition
energy quotient
subclinical ketosis identification
url https://acta.mendelu.cz/61/6/1661/
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