The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i>
Histones are the core components of the eukaryote chromosome, and have been implicated in transcriptional gene regulation. There are three major isoforms of histone H3 in <i>Arabidopsis</i>. Studies have shown that the H3.3 variant is pivotal in modulating nucleosome structure and gene t...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/5/2549 |
| _version_ | 1797264471505240064 |
|---|---|
| author | Xiaogang Long Wandong Yang Yanfang Lv Xiaoming Zhong Lin Chen Qingzhu Li Zhaopeng Lv Yanzhuo Li Yajun Cai Hongchun Yang |
| author_facet | Xiaogang Long Wandong Yang Yanfang Lv Xiaoming Zhong Lin Chen Qingzhu Li Zhaopeng Lv Yanzhuo Li Yajun Cai Hongchun Yang |
| author_sort | Xiaogang Long |
| collection | DOAJ |
| description | Histones are the core components of the eukaryote chromosome, and have been implicated in transcriptional gene regulation. There are three major isoforms of histone H3 in <i>Arabidopsis</i>. Studies have shown that the H3.3 variant is pivotal in modulating nucleosome structure and gene transcription. However, the function of H3.3 during development remains to be further investigated in plants. In this study, we disrupted all three <i>H3.3</i> genes in <i>Arabidopsis</i>. Two triple mutants, <i>h3.3cr-4</i> and <i>h3.3cr-5,</i> were created by the CRISPR/Cas9 system. The mutant plants displayed smaller rosettes and decreased fertility. The stunted growth of <i>h3.3cr-4</i> may result from reduced expression of cell cycle regulators. The shorter stamen filaments, but not the fertile ability of the gametophytes, resulted in reduced fertility of <i>h3.3cr-4</i>. The transcriptome analysis suggested that the reduced filament elongation of <i>h3.3cr-4</i> was probably caused by the ectopic expression of several <i>JASMONATE-ZIM DOMAIN</i> (<i>JAZ</i>) genes, which are the key repressors of the signaling pathway of the phytohormone jasmonic acid (JA). These observations suggest that the histone variant H3.3 promotes plant growth, including rosette growth and filament elongation. |
| first_indexed | 2024-04-25T00:29:26Z |
| format | Article |
| id | doaj.art-d32b948486584c4abfa9734f9ecdc015 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:29:26Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d32b948486584c4abfa9734f9ecdc0152024-03-12T16:45:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01255254910.3390/ijms25052549The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i>Xiaogang Long0Wandong Yang1Yanfang Lv2Xiaoming Zhong3Lin Chen4Qingzhu Li5Zhaopeng Lv6Yanzhuo Li7Yajun Cai8Hongchun Yang9State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaHistones are the core components of the eukaryote chromosome, and have been implicated in transcriptional gene regulation. There are three major isoforms of histone H3 in <i>Arabidopsis</i>. Studies have shown that the H3.3 variant is pivotal in modulating nucleosome structure and gene transcription. However, the function of H3.3 during development remains to be further investigated in plants. In this study, we disrupted all three <i>H3.3</i> genes in <i>Arabidopsis</i>. Two triple mutants, <i>h3.3cr-4</i> and <i>h3.3cr-5,</i> were created by the CRISPR/Cas9 system. The mutant plants displayed smaller rosettes and decreased fertility. The stunted growth of <i>h3.3cr-4</i> may result from reduced expression of cell cycle regulators. The shorter stamen filaments, but not the fertile ability of the gametophytes, resulted in reduced fertility of <i>h3.3cr-4</i>. The transcriptome analysis suggested that the reduced filament elongation of <i>h3.3cr-4</i> was probably caused by the ectopic expression of several <i>JASMONATE-ZIM DOMAIN</i> (<i>JAZ</i>) genes, which are the key repressors of the signaling pathway of the phytohormone jasmonic acid (JA). These observations suggest that the histone variant H3.3 promotes plant growth, including rosette growth and filament elongation.https://www.mdpi.com/1422-0067/25/5/2549<i>Arabidopsis</i>histone variant H3.3rosette growthfilament elongation<i>JAZ</i> |
| spellingShingle | Xiaogang Long Wandong Yang Yanfang Lv Xiaoming Zhong Lin Chen Qingzhu Li Zhaopeng Lv Yanzhuo Li Yajun Cai Hongchun Yang The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i> International Journal of Molecular Sciences <i>Arabidopsis</i> histone variant H3.3 rosette growth filament elongation <i>JAZ</i> |
| title | The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i> |
| title_full | The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i> |
| title_fullStr | The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i> |
| title_full_unstemmed | The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i> |
| title_short | The Histone Variant H3.3 Is Required for Plant Growth and Fertility in <i>Arabidopsis</i> |
| title_sort | histone variant h3 3 is required for plant growth and fertility in i arabidopsis i |
| topic | <i>Arabidopsis</i> histone variant H3.3 rosette growth filament elongation <i>JAZ</i> |
| url | https://www.mdpi.com/1422-0067/25/5/2549 |
| work_keys_str_mv | AT xiaoganglong thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT wandongyang thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT yanfanglv thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT xiaomingzhong thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT linchen thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT qingzhuli thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT zhaopenglv thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT yanzhuoli thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT yajuncai thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT hongchunyang thehistonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT xiaoganglong histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT wandongyang histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT yanfanglv histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT xiaomingzhong histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT linchen histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT qingzhuli histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT zhaopenglv histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT yanzhuoli histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT yajuncai histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi AT hongchunyang histonevarianth33isrequiredforplantgrowthandfertilityiniarabidopsisi |