Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in Keratinocytes
Hye Ran Kim,1 So Yeon Lee,1 Ga Eun You,2 Hye One Kim,1 Chun Wook Park,1 Bo Young Chung1 1Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, 07441, Korea; 2Research and Development Institute, Biosolution, Seoul Technopark, Seoul,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-12-01
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| Series: | Clinical, Cosmetic and Investigational Dermatology |
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| Online Access: | https://www.dovepress.com/adipose-derived-stem-cell-exosomes-alleviate-psoriasis-serum-exosomes--peer-reviewed-fulltext-article-CCID |
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| author | Kim HR Lee SY You GE Kim HO Park CW Chung BY |
| author_facet | Kim HR Lee SY You GE Kim HO Park CW Chung BY |
| author_sort | Kim HR |
| collection | DOAJ |
| description | Hye Ran Kim,1 So Yeon Lee,1 Ga Eun You,2 Hye One Kim,1 Chun Wook Park,1 Bo Young Chung1 1Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, 07441, Korea; 2Research and Development Institute, Biosolution, Seoul Technopark, Seoul, 01811, KoreaCorrespondence: Bo Young Chung, Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 1 Singil-ro, Yeongdeungpo-gu, Seoul, 07441, Korea, Tel +82-2-829-5221, Fax +82-2-832-3237, Email victoryby@hallym.or.krIntroduction: Exosomes play a key role in cell communication and are involved in both pathological and physiological processes. Autophagy dysfunction and oxidative stress are linked to immune-mediated inflammatory diseases such as psoriasis. Stem cell-derived exosomes exhibit immunomodulatory and antioxidant efficacy.Methods: We aimed to investigate the impact of psoriasis serum-derived exosomes on inflammation, oxidative stress, and autophagy in keratinocytes. Additionally, we explored the therapeutic potential of adipose-derived stem cell (ADSC) exosomes against inflammation induced by psoriasis serum exosomes. To validate psoriasis patient serum-derived exosomes and ADSC exosomes, we used nanoparticle tracking analysis, Western blotting, flow cytometry, and immunofluorescence. qPCR was used to study changes in the gene expression of proinflammatory cytokines and oxidative stress markers in HaCaT cells treated with psoriasis serum-derived exosomes or ADSC exosomes. The effects of these exosomes on autophagy in HaCaT cells were evaluated by Western blotting and immunofluorescence.Result: The treatment of HaCaT cells with psoriasis serum-derived exosomes increased proinflammatory cytokine production and oxidative stress-related factor (Nox2 and Nox4) expression and decreased Nrf2 expression via P65/NF-κB and P38/MAPK activation. Compared with healthy control serum-derived exosomes, psoriasis serum-derived exosomes decreased ATG5, P62, Beclin1, and LC3 expression and autophagosome production in HaCaT cells. Conversely, ADSC exosomes suppressed proinflammatory cytokine and oxidative stress production, and restored autophagy in HaCaT cells treated with psoriasis serum-derived exosomes.Discussion: These findings suggest that ADSC exosomes exhibit a suppressive effect on psoriasis serum exosome-induced inflammation and oxidative stress by regulating autophagy in keratinocytes.Keywords: exosomes, adipose-derived stem cell, psoriasis, autophagy, oxidative stress |
| first_indexed | 2024-03-08T19:22:54Z |
| format | Article |
| id | doaj.art-d32dde4219454b6b9d4592cdb3125bf6 |
| institution | Directory Open Access Journal |
| issn | 1178-7015 |
| language | English |
| last_indexed | 2024-03-08T19:22:54Z |
| publishDate | 2023-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj.art-d32dde4219454b6b9d4592cdb3125bf62023-12-26T18:05:30ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152023-12-01Volume 163699371189273Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in KeratinocytesKim HRLee SYYou GEKim HOPark CWChung BYHye Ran Kim,1 So Yeon Lee,1 Ga Eun You,2 Hye One Kim,1 Chun Wook Park,1 Bo Young Chung1 1Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, 07441, Korea; 2Research and Development Institute, Biosolution, Seoul Technopark, Seoul, 01811, KoreaCorrespondence: Bo Young Chung, Department of Dermatology, Hallym University Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 1 Singil-ro, Yeongdeungpo-gu, Seoul, 07441, Korea, Tel +82-2-829-5221, Fax +82-2-832-3237, Email victoryby@hallym.or.krIntroduction: Exosomes play a key role in cell communication and are involved in both pathological and physiological processes. Autophagy dysfunction and oxidative stress are linked to immune-mediated inflammatory diseases such as psoriasis. Stem cell-derived exosomes exhibit immunomodulatory and antioxidant efficacy.Methods: We aimed to investigate the impact of psoriasis serum-derived exosomes on inflammation, oxidative stress, and autophagy in keratinocytes. Additionally, we explored the therapeutic potential of adipose-derived stem cell (ADSC) exosomes against inflammation induced by psoriasis serum exosomes. To validate psoriasis patient serum-derived exosomes and ADSC exosomes, we used nanoparticle tracking analysis, Western blotting, flow cytometry, and immunofluorescence. qPCR was used to study changes in the gene expression of proinflammatory cytokines and oxidative stress markers in HaCaT cells treated with psoriasis serum-derived exosomes or ADSC exosomes. The effects of these exosomes on autophagy in HaCaT cells were evaluated by Western blotting and immunofluorescence.Result: The treatment of HaCaT cells with psoriasis serum-derived exosomes increased proinflammatory cytokine production and oxidative stress-related factor (Nox2 and Nox4) expression and decreased Nrf2 expression via P65/NF-κB and P38/MAPK activation. Compared with healthy control serum-derived exosomes, psoriasis serum-derived exosomes decreased ATG5, P62, Beclin1, and LC3 expression and autophagosome production in HaCaT cells. Conversely, ADSC exosomes suppressed proinflammatory cytokine and oxidative stress production, and restored autophagy in HaCaT cells treated with psoriasis serum-derived exosomes.Discussion: These findings suggest that ADSC exosomes exhibit a suppressive effect on psoriasis serum exosome-induced inflammation and oxidative stress by regulating autophagy in keratinocytes.Keywords: exosomes, adipose-derived stem cell, psoriasis, autophagy, oxidative stresshttps://www.dovepress.com/adipose-derived-stem-cell-exosomes-alleviate-psoriasis-serum-exosomes--peer-reviewed-fulltext-article-CCIDexosomesadipose-derived stem cellpsoriasisautophagyoxidative stress |
| spellingShingle | Kim HR Lee SY You GE Kim HO Park CW Chung BY Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in Keratinocytes Clinical, Cosmetic and Investigational Dermatology exosomes adipose-derived stem cell psoriasis autophagy oxidative stress |
| title | Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in Keratinocytes |
| title_full | Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in Keratinocytes |
| title_fullStr | Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in Keratinocytes |
| title_full_unstemmed | Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in Keratinocytes |
| title_short | Adipose-Derived Stem Cell Exosomes Alleviate Psoriasis Serum Exosomes-Induced Inflammation by Regulating Autophagy and Redox Status in Keratinocytes |
| title_sort | adipose derived stem cell exosomes alleviate psoriasis serum exosomes induced inflammation by regulating autophagy and redox status in keratinocytes |
| topic | exosomes adipose-derived stem cell psoriasis autophagy oxidative stress |
| url | https://www.dovepress.com/adipose-derived-stem-cell-exosomes-alleviate-psoriasis-serum-exosomes--peer-reviewed-fulltext-article-CCID |
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