Higher interleukin-33 levels in aggressive periodontitis cases
Context: Interleukin-33 (IL-33) is a novel alarmin that warns immune cells of tissue destruction in injury or infection. Aims: This study is aimed at analyzing and correlating the concentration of IL-33 in the gingival crevicular fluid (GCF) and plasma of healthy subjects and chronic and aggressive...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2019-01-01
|
| Series: | Journal of Indian Society of Periodontology |
| Subjects: | |
| Online Access: | http://www.jisponline.com/article.asp?issn=0972-124X;year=2019;volume=23;issue=5;spage=424;epage=429;aulast=Ballambettu |
| _version_ | 1811263784564555776 |
|---|---|
| author | Sujatha Pai Ballambettu A R Pradeep Meera Purushottam Somdatta Sen |
| author_facet | Sujatha Pai Ballambettu A R Pradeep Meera Purushottam Somdatta Sen |
| author_sort | Sujatha Pai Ballambettu |
| collection | DOAJ |
| description | Context: Interleukin-33 (IL-33) is a novel alarmin that warns immune cells of tissue destruction in injury or infection. Aims: This study is aimed at analyzing and correlating the concentration of IL-33 in the gingival crevicular fluid (GCF) and plasma of healthy subjects and chronic and aggressive periodontitis patients with intronic variant single nucleotide polymorphisms (SNPs), rs1157505 (C/G) and rs7044343 (C/T) in the IL-33 gene. Settings and Design: This is a cross-sectional, biochemical, genetic study. Materials and Methods: Ninety subjects were divided into Group H: subjects with healthy periodontium, Group CP: chronic periodontitis patients, and Group GAgP: generalized aggressive periodontitis patients, based on clinical parameters of periodontal inflammation. IL-33 concentration in GCF, as well as plasma, was quantified using enzyme-linked immunosorbent assay. The SNPs rs1157505 and rs7044343 were analyzed using polymerase chain reaction-restriction fragment length polymorphism. Results: A significant difference was found in IL-33 concentration in GCF and plasma between the three groups. GG genotype of IL-33 SNP rs1157505 was associated with the highest GCF and plasma IL-33 concentration and was significantly more in GAgP than healthy or CP groups. IL-33 SNP rs7044343 did not show any such association. All GAgP patients had the highest GCF and plasma concentration of IL-33. Conclusions: IL-33 may be a potential inflammatory marker of periodontitis. GG genotype of SNP rs1157505 may be associated with GAgP. |
| first_indexed | 2024-04-12T19:51:28Z |
| format | Article |
| id | doaj.art-d32ea380383548af9f81cbc315043cc3 |
| institution | Directory Open Access Journal |
| issn | 0972-124X |
| language | English |
| last_indexed | 2024-04-12T19:51:28Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Periodontology |
| spelling | doaj.art-d32ea380383548af9f81cbc315043cc32022-12-22T03:18:49ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2019-01-0123542442910.4103/jisp.jisp_217_19Higher interleukin-33 levels in aggressive periodontitis casesSujatha Pai BallambettuA R PradeepMeera PurushottamSomdatta SenContext: Interleukin-33 (IL-33) is a novel alarmin that warns immune cells of tissue destruction in injury or infection. Aims: This study is aimed at analyzing and correlating the concentration of IL-33 in the gingival crevicular fluid (GCF) and plasma of healthy subjects and chronic and aggressive periodontitis patients with intronic variant single nucleotide polymorphisms (SNPs), rs1157505 (C/G) and rs7044343 (C/T) in the IL-33 gene. Settings and Design: This is a cross-sectional, biochemical, genetic study. Materials and Methods: Ninety subjects were divided into Group H: subjects with healthy periodontium, Group CP: chronic periodontitis patients, and Group GAgP: generalized aggressive periodontitis patients, based on clinical parameters of periodontal inflammation. IL-33 concentration in GCF, as well as plasma, was quantified using enzyme-linked immunosorbent assay. The SNPs rs1157505 and rs7044343 were analyzed using polymerase chain reaction-restriction fragment length polymorphism. Results: A significant difference was found in IL-33 concentration in GCF and plasma between the three groups. GG genotype of IL-33 SNP rs1157505 was associated with the highest GCF and plasma IL-33 concentration and was significantly more in GAgP than healthy or CP groups. IL-33 SNP rs7044343 did not show any such association. All GAgP patients had the highest GCF and plasma concentration of IL-33. Conclusions: IL-33 may be a potential inflammatory marker of periodontitis. GG genotype of SNP rs1157505 may be associated with GAgP.http://www.jisponline.com/article.asp?issn=0972-124X;year=2019;volume=23;issue=5;spage=424;epage=429;aulast=BallambettuAggressive periodontitischronic periodontitisgenetic polymorphismgingival crevicular fluidinterleukin |
| spellingShingle | Sujatha Pai Ballambettu A R Pradeep Meera Purushottam Somdatta Sen Higher interleukin-33 levels in aggressive periodontitis cases Journal of Indian Society of Periodontology Aggressive periodontitis chronic periodontitis genetic polymorphism gingival crevicular fluid interleukin |
| title | Higher interleukin-33 levels in aggressive periodontitis cases |
| title_full | Higher interleukin-33 levels in aggressive periodontitis cases |
| title_fullStr | Higher interleukin-33 levels in aggressive periodontitis cases |
| title_full_unstemmed | Higher interleukin-33 levels in aggressive periodontitis cases |
| title_short | Higher interleukin-33 levels in aggressive periodontitis cases |
| title_sort | higher interleukin 33 levels in aggressive periodontitis cases |
| topic | Aggressive periodontitis chronic periodontitis genetic polymorphism gingival crevicular fluid interleukin |
| url | http://www.jisponline.com/article.asp?issn=0972-124X;year=2019;volume=23;issue=5;spage=424;epage=429;aulast=Ballambettu |
| work_keys_str_mv | AT sujathapaiballambettu higherinterleukin33levelsinaggressiveperiodontitiscases AT arpradeep higherinterleukin33levelsinaggressiveperiodontitiscases AT meerapurushottam higherinterleukin33levelsinaggressiveperiodontitiscases AT somdattasen higherinterleukin33levelsinaggressiveperiodontitiscases |