A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan
Abstract Background The timing of bud break is very important for the flowering and fruiting of longan. To obtain new insights into the underlying regulatory mechanism of bud break in longan, a comparative analysis was conducted in three flower induction stages of two longan varieties with opposite...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-10-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03868-3 |
| _version_ | 1798030015677333504 |
|---|---|
| author | Dengwei Jue Liqin Liu Xuelian Sang Shengyou Shi |
| author_facet | Dengwei Jue Liqin Liu Xuelian Sang Shengyou Shi |
| author_sort | Dengwei Jue |
| collection | DOAJ |
| description | Abstract Background The timing of bud break is very important for the flowering and fruiting of longan. To obtain new insights into the underlying regulatory mechanism of bud break in longan, a comparative analysis was conducted in three flower induction stages of two longan varieties with opposite flowering phenotypes by using isobaric tags for relative and absolute quantification (iTRAQ). Results In total, 3180 unique proteins were identified in 18 samples, and 1101 differentially abundant proteins (DAPs) were identified. “SX” (“Shixia”), a common longan cultivated variety that needs an appropriate period of low temperatures to accumulate energy and nutrients for flower induction, had a strong primary inflorescence, had a strong axillary inflorescence, and contained high contents of sugars, and most DAPs during the bud break process were enriched in assimilates and energy metabolism. Combined with our previous transcriptome data, it was observed that sucrose synthase 6 (SS6) and granule-bound starch synthase 1 (GBSSI) might be the key DAPs for “SX” bud break. Compared to those of “SX”, the primary inflorescence, axillary inflorescence, floral primordium, bract, and prophyll of “SJ” (“Sijimi”) were weaker. In addition, light, rather than a high sugar content or chilling duration, might act as the key signal for triggering bud break. In addition, catalase isozyme 1, an important enzyme in the redox cycle, and RuBisCO, a key enzyme in the Calvin cycle of photosynthetic carbon assimilation, might be the key DAPs for SJ bud break. Conclusion Our results present a dynamic picture of the bud break of longan, not only revealing the temporal specific expression of key candidate genes and proteins but also providing a scientific basis for the genetic improvement of this fruit tree species. |
| first_indexed | 2024-04-11T19:33:28Z |
| format | Article |
| id | doaj.art-d32eacb0b34747099bf9d0dbc4b50609 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-11T19:33:28Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-d32eacb0b34747099bf9d0dbc4b506092022-12-22T04:06:56ZengBMCBMC Plant Biology1471-22292022-10-0122111310.1186/s12870-022-03868-3A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longanDengwei Jue0Liqin Liu1Xuelian Sang2Shengyou Shi3Chongqing Key Laboratory of Economic Plant Biotechnology, Collaborative Innovation Center of Special Plant Industry in Chongqing, Chongqing Engineering Research Center for Special Plant Seedling, Institute of Special Plants, Chongqing University of Arts and SciencesKey Laboratory of Tropical Fruit Biology (Ministry of Agriculture), South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural SciencesChongqing Key Laboratory of Economic Plant Biotechnology, Collaborative Innovation Center of Special Plant Industry in Chongqing, Chongqing Engineering Research Center for Special Plant Seedling, Institute of Special Plants, Chongqing University of Arts and SciencesKey Laboratory of Tropical Fruit Biology (Ministry of Agriculture), South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural SciencesAbstract Background The timing of bud break is very important for the flowering and fruiting of longan. To obtain new insights into the underlying regulatory mechanism of bud break in longan, a comparative analysis was conducted in three flower induction stages of two longan varieties with opposite flowering phenotypes by using isobaric tags for relative and absolute quantification (iTRAQ). Results In total, 3180 unique proteins were identified in 18 samples, and 1101 differentially abundant proteins (DAPs) were identified. “SX” (“Shixia”), a common longan cultivated variety that needs an appropriate period of low temperatures to accumulate energy and nutrients for flower induction, had a strong primary inflorescence, had a strong axillary inflorescence, and contained high contents of sugars, and most DAPs during the bud break process were enriched in assimilates and energy metabolism. Combined with our previous transcriptome data, it was observed that sucrose synthase 6 (SS6) and granule-bound starch synthase 1 (GBSSI) might be the key DAPs for “SX” bud break. Compared to those of “SX”, the primary inflorescence, axillary inflorescence, floral primordium, bract, and prophyll of “SJ” (“Sijimi”) were weaker. In addition, light, rather than a high sugar content or chilling duration, might act as the key signal for triggering bud break. In addition, catalase isozyme 1, an important enzyme in the redox cycle, and RuBisCO, a key enzyme in the Calvin cycle of photosynthetic carbon assimilation, might be the key DAPs for SJ bud break. Conclusion Our results present a dynamic picture of the bud break of longan, not only revealing the temporal specific expression of key candidate genes and proteins but also providing a scientific basis for the genetic improvement of this fruit tree species.https://doi.org/10.1186/s12870-022-03868-3LonganFlower bud breakiTRAQCarbohydratePhotosynthesis |
| spellingShingle | Dengwei Jue Liqin Liu Xuelian Sang Shengyou Shi A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan BMC Plant Biology Longan Flower bud break iTRAQ Carbohydrate Photosynthesis |
| title | A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan |
| title_full | A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan |
| title_fullStr | A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan |
| title_full_unstemmed | A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan |
| title_short | A comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan |
| title_sort | comparative proteomic analysis provides insight into the molecular mechanism of bud break in longan |
| topic | Longan Flower bud break iTRAQ Carbohydrate Photosynthesis |
| url | https://doi.org/10.1186/s12870-022-03868-3 |
| work_keys_str_mv | AT dengweijue acomparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan AT liqinliu acomparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan AT xueliansang acomparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan AT shengyoushi acomparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan AT dengweijue comparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan AT liqinliu comparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan AT xueliansang comparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan AT shengyoushi comparativeproteomicanalysisprovidesinsightintothemolecularmechanismofbudbreakinlongan |