Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis
Abstract Arabinase is an enzyme recognized for its ability to degrade arabinan, a plant cell wall constituent. It has been applied in the food industry most commonly for juice processing. One commercial source of arabinase is Aspergillus tubingensis (A. tubingensis), a black Aspergillus species. Giv...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.1329 |
| _version_ | 1797221380924637184 |
|---|---|
| author | Nobuo Okado Mai Sugi Sawako Kasamoto Fukutaro Mizuhashi Ashley Roberts Barbara Danielewska‐Nikiel Christina Sulaiman Sa Pham |
| author_facet | Nobuo Okado Mai Sugi Sawako Kasamoto Fukutaro Mizuhashi Ashley Roberts Barbara Danielewska‐Nikiel Christina Sulaiman Sa Pham |
| author_sort | Nobuo Okado |
| collection | DOAJ |
| description | Abstract Arabinase is an enzyme recognized for its ability to degrade arabinan, a plant cell wall constituent. It has been applied in the food industry most commonly for juice processing. One commercial source of arabinase is Aspergillus tubingensis (A. tubingensis), a black Aspergillus species. Given the intended use in food for human consumption, and noting its potential presence at trace levels in finished products, a series of safety studies including in vitro Ames and chromosome aberration assays, in vivo mammalian erythrocyte micronucleus and alkaline comet assays, and a 90‐day rat oral toxicity study were conducted. No test article‐related mutagenic activity was observed in the Ames assay. Although positive activity was observed in the chromosome aberration assay, this was not replicated in the in vivo genotoxicity assays including in preabsorptive cells. In the subchronic toxicity study, no test article‐related adverse effects were observed following oral administration of arabinase at doses of 15.3, 153, or 1,530 mg total organic solids (TOS)/kg body weight/day to Sprague Dawley rats. The no‐observed‐adverse‐effect level was considered to be the highest dose tested (1,530 mg TOS/kg body weight/day). The results of the genotoxicity studies and the subchronic toxicity study support the safe use of arabinase from A. tubingensis in food production. |
| first_indexed | 2024-04-24T13:04:31Z |
| format | Article |
| id | doaj.art-d33158e45d44410e825e8ee84ad88a4c |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-24T13:04:31Z |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-d33158e45d44410e825e8ee84ad88a4c2024-04-05T09:16:03ZengWileyFood Science & Nutrition2048-71772020-01-018145647810.1002/fsn3.1329Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensisNobuo Okado0Mai Sugi1Sawako Kasamoto2Fukutaro Mizuhashi3Ashley Roberts4Barbara Danielewska‐Nikiel5Christina Sulaiman6Sa Pham7Shin Nihon Chemical Co., Ltd Aichi JapanBiosafety Research Center, Foods, Drugs, and Pesticides (currently BioSafety Research Center Inc.) Shizuoka JapanBiosafety Research Center, Foods, Drugs, and Pesticides (currently BioSafety Research Center Inc.) Shizuoka JapanBiosafety Research Center, Foods, Drugs, and Pesticides (currently BioSafety Research Center Inc.) Shizuoka JapanIntertek Scientific and Regulatory Consultancy Mississauga ON CanadaIntertek Scientific and Regulatory Consultancy Mississauga ON CanadaIntertek Scientific and Regulatory Consultancy Mississauga ON CanadaIntertek Scientific and Regulatory Consultancy Mississauga ON CanadaAbstract Arabinase is an enzyme recognized for its ability to degrade arabinan, a plant cell wall constituent. It has been applied in the food industry most commonly for juice processing. One commercial source of arabinase is Aspergillus tubingensis (A. tubingensis), a black Aspergillus species. Given the intended use in food for human consumption, and noting its potential presence at trace levels in finished products, a series of safety studies including in vitro Ames and chromosome aberration assays, in vivo mammalian erythrocyte micronucleus and alkaline comet assays, and a 90‐day rat oral toxicity study were conducted. No test article‐related mutagenic activity was observed in the Ames assay. Although positive activity was observed in the chromosome aberration assay, this was not replicated in the in vivo genotoxicity assays including in preabsorptive cells. In the subchronic toxicity study, no test article‐related adverse effects were observed following oral administration of arabinase at doses of 15.3, 153, or 1,530 mg total organic solids (TOS)/kg body weight/day to Sprague Dawley rats. The no‐observed‐adverse‐effect level was considered to be the highest dose tested (1,530 mg TOS/kg body weight/day). The results of the genotoxicity studies and the subchronic toxicity study support the safe use of arabinase from A. tubingensis in food production.https://doi.org/10.1002/fsn3.1329ArabinaseAspergillus tubingensisfood safetygenotoxicitysubchronic toxicity |
| spellingShingle | Nobuo Okado Mai Sugi Sawako Kasamoto Fukutaro Mizuhashi Ashley Roberts Barbara Danielewska‐Nikiel Christina Sulaiman Sa Pham Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis Food Science & Nutrition Arabinase Aspergillus tubingensis food safety genotoxicity subchronic toxicity |
| title | Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis |
| title_full | Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis |
| title_fullStr | Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis |
| title_full_unstemmed | Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis |
| title_short | Safety evaluation of arabinase (arabinan endo‐1,5‐α‐L‐arabinanase) from Aspergillus tubingensis |
| title_sort | safety evaluation of arabinase arabinan endo 1 5 α l arabinanase from aspergillus tubingensis |
| topic | Arabinase Aspergillus tubingensis food safety genotoxicity subchronic toxicity |
| url | https://doi.org/10.1002/fsn3.1329 |
| work_keys_str_mv | AT nobuookado safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis AT maisugi safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis AT sawakokasamoto safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis AT fukutaromizuhashi safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis AT ashleyroberts safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis AT barbaradanielewskanikiel safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis AT christinasulaiman safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis AT sapham safetyevaluationofarabinasearabinanendo15alarabinanasefromaspergillustubingensis |