Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis
D-Glyceric acid (D-GA) is a promising chemical for minimizing the toxic effect of acetaldehyde. It has been successfully produced by bioprocesses using glycerol as a substrate. However, high concentrations of glycerol are not beneficial for cell growth. A two-step culture strategy was employed to de...
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Croatian Society of Chemical Engineers
2018-03-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
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| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/13-1-18.pdf |
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| author | B. Wang Y. Pu H. G. Gerken Y. Xie L. Lin H. Chen Y. Lu |
| author_facet | B. Wang Y. Pu H. G. Gerken Y. Xie L. Lin H. Chen Y. Lu |
| author_sort | B. Wang |
| collection | DOAJ |
| description | D-Glyceric acid (D-GA) is a promising chemical for minimizing the toxic effect of acetaldehyde. It has been successfully produced by bioprocesses using glycerol as a substrate. However, high concentrations of glycerol are not beneficial for cell growth. A two-step culture strategy was employed to deal with the ambivalent culture conditions between the growth of Acetobacter tropicalis and the biosynthesis of D-GA. The first stage focuses on biomass accumulation with low initial glycerol concentration; the second stage provides D-GA accumulation through whole-cell biocatalysis. Approximately 2-fold of D-GA yield and 4.5-fold of D-GA productivity were gained by the two-step culture strategy compared to the traditional fermentation with 150 g L–1 of glycerol. Our results also showed that A. tropicalis was able to catalyze the conversion of glycerol to D-GA in the presence of up to 10 % methanol. These findings have important implications to enhance D-GA yield by strategy optimization and reduce its cost. |
| first_indexed | 2024-12-12T19:36:57Z |
| format | Article |
| id | doaj.art-d33990b67d654df1b982c8206a7cbbb6 |
| institution | Directory Open Access Journal |
| issn | 0352-9568 1846-5153 |
| language | English |
| last_indexed | 2024-12-12T19:36:57Z |
| publishDate | 2018-03-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Chemical and Biochemical Engineering Quarterly |
| spelling | doaj.art-d33990b67d654df1b982c8206a7cbbb62022-12-22T00:14:18ZengCroatian Society of Chemical EngineersChemical and Biochemical Engineering Quarterly0352-95681846-51532018-03-0132113514010.15255/CABEQ.2017.1181Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalisB. Wang0 Y. Pu1H. G. Gerken2Y. Xie3 L. Lin4H. Chen5Y. Lu6a) College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, China, e) Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou 362000, ChinaCollege of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaArizona Center for Algal Technology and Innovation, Arizona State University, Mesa, Arizona 85212, USCollege of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, ChinaCollege of Oceanology and Food Science, Quanzhou Normal University, Quanzhou 362000, ChinaCollege of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaCollege of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, ChinaD-Glyceric acid (D-GA) is a promising chemical for minimizing the toxic effect of acetaldehyde. It has been successfully produced by bioprocesses using glycerol as a substrate. However, high concentrations of glycerol are not beneficial for cell growth. A two-step culture strategy was employed to deal with the ambivalent culture conditions between the growth of Acetobacter tropicalis and the biosynthesis of D-GA. The first stage focuses on biomass accumulation with low initial glycerol concentration; the second stage provides D-GA accumulation through whole-cell biocatalysis. Approximately 2-fold of D-GA yield and 4.5-fold of D-GA productivity were gained by the two-step culture strategy compared to the traditional fermentation with 150 g L–1 of glycerol. Our results also showed that A. tropicalis was able to catalyze the conversion of glycerol to D-GA in the presence of up to 10 % methanol. These findings have important implications to enhance D-GA yield by strategy optimization and reduce its cost.http://silverstripe.fkit.hr/cabeq/assets/Uploads/13-1-18.pdfglyceric acidAcetobactertwo-step culturebiocatalysisfermentationcost-effective production |
| spellingShingle | B. Wang Y. Pu H. G. Gerken Y. Xie L. Lin H. Chen Y. Lu Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis Chemical and Biochemical Engineering Quarterly glyceric acid Acetobacter two-step culture biocatalysis fermentation cost-effective production |
| title | Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis |
| title_full | Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis |
| title_fullStr | Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis |
| title_full_unstemmed | Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis |
| title_short | Production of D-Glyceric Acid by a Two-step Culture Strategy Based on Whole-cell Biocatalysis of Acetobacter tropicalis |
| title_sort | production of d glyceric acid by a two step culture strategy based on whole cell biocatalysis of acetobacter tropicalis |
| topic | glyceric acid Acetobacter two-step culture biocatalysis fermentation cost-effective production |
| url | http://silverstripe.fkit.hr/cabeq/assets/Uploads/13-1-18.pdf |
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