Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis
N-Acetylneuraminic acid (Neu5Ac), the most common type of Sia, generally acts as the terminal sugar in cell surface glycans, glycoconjugates, oligosaccharides, lipo-oligosaccharides, and polysaccharides, thus exerting numerous physiological functions. The extensive applications of Neu5Ac in the food...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Synthetic and Systems Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X23000546 |
| _version_ | 1827267951919628288 |
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| author | Mingli Zhao Yingying Zhu Hao Wang Wenli Zhang Wanmeng Mu |
| author_facet | Mingli Zhao Yingying Zhu Hao Wang Wenli Zhang Wanmeng Mu |
| author_sort | Mingli Zhao |
| collection | DOAJ |
| description | N-Acetylneuraminic acid (Neu5Ac), the most common type of Sia, generally acts as the terminal sugar in cell surface glycans, glycoconjugates, oligosaccharides, lipo-oligosaccharides, and polysaccharides, thus exerting numerous physiological functions. The extensive applications of Neu5Ac in the food, cosmetic, and pharmaceutical industries make large-scale production of this chemical desirable. Biosynthesis which is associated with important application potential and environmental friendliness has become an indispensable approach for large-scale synthesis of Neu5Ac. In this review, the physiological roles of Neu5Ac was first summarized in detail. Second, the safety evaluation, regulatory status, and applications of Neu5Ac were discussed. Third, enzyme-catalyzed preparation, whole-cell biocatalysis, and microbial de novo synthesis of Neu5Ac were comprehensively reviewed. In addition, we discussed the main challenges of Neu5Ac de novo biosynthesis, such as screening and engineering of key enzymes, identifying exporters of intermediates and Neu5Ac, and balancing cell growth and biosynthesis. The corresponding strategies and systematic strategies were proposed to overcome these challenges and facilitate Neu5Ac industrial-scale production. |
| first_indexed | 2024-03-12T11:02:54Z |
| format | Article |
| id | doaj.art-d33acb85ab86458090dc3a107d819369 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2025-03-22T04:41:54Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-d33acb85ab86458090dc3a107d8193692024-04-27T22:13:00ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2023-09-0183509519Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesisMingli Zhao0Yingying Zhu1Hao Wang2Wenli Zhang3Wanmeng Mu4State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, PR ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, PR ChinaBloomage Biotechnology Corp., Ltd., Jinan, Shandong, 250010, PR ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, PR ChinaState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, PR China; Corresponding author. State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, PR China.N-Acetylneuraminic acid (Neu5Ac), the most common type of Sia, generally acts as the terminal sugar in cell surface glycans, glycoconjugates, oligosaccharides, lipo-oligosaccharides, and polysaccharides, thus exerting numerous physiological functions. The extensive applications of Neu5Ac in the food, cosmetic, and pharmaceutical industries make large-scale production of this chemical desirable. Biosynthesis which is associated with important application potential and environmental friendliness has become an indispensable approach for large-scale synthesis of Neu5Ac. In this review, the physiological roles of Neu5Ac was first summarized in detail. Second, the safety evaluation, regulatory status, and applications of Neu5Ac were discussed. Third, enzyme-catalyzed preparation, whole-cell biocatalysis, and microbial de novo synthesis of Neu5Ac were comprehensively reviewed. In addition, we discussed the main challenges of Neu5Ac de novo biosynthesis, such as screening and engineering of key enzymes, identifying exporters of intermediates and Neu5Ac, and balancing cell growth and biosynthesis. The corresponding strategies and systematic strategies were proposed to overcome these challenges and facilitate Neu5Ac industrial-scale production.http://www.sciencedirect.com/science/article/pii/S2405805X23000546N-Acetylneuraminic acidSiaPhysiological effectsBiosynthesisMetabolic engineering strategies |
| spellingShingle | Mingli Zhao Yingying Zhu Hao Wang Wenli Zhang Wanmeng Mu Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis Synthetic and Systems Biotechnology N-Acetylneuraminic acid Sia Physiological effects Biosynthesis Metabolic engineering strategies |
| title | Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis |
| title_full | Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis |
| title_fullStr | Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis |
| title_full_unstemmed | Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis |
| title_short | Recent advances on N-acetylneuraminic acid: Physiological roles, applications, and biosynthesis |
| title_sort | recent advances on n acetylneuraminic acid physiological roles applications and biosynthesis |
| topic | N-Acetylneuraminic acid Sia Physiological effects Biosynthesis Metabolic engineering strategies |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X23000546 |
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