RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic density
Abstract Background Wood basic density (WBD), the biomass of plant cell walls per unit volume, is an important trait for elite tree selection in kraft pulp production. Here, we investigated the correlation between WBD and wood volumes or wood properties using 98 open-pollinated, 2.4 to 2.8 year-old...
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-018-1371-9 |
| _version_ | 1818940806772293632 |
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| author | Katsuhiko Nakahama Nobuaki Urata Tomotaka Shinya Kazunori Hayashi Kazuya Nanto Antonio C. Rosa Akiyoshi Kawaoka |
| author_facet | Katsuhiko Nakahama Nobuaki Urata Tomotaka Shinya Kazunori Hayashi Kazuya Nanto Antonio C. Rosa Akiyoshi Kawaoka |
| author_sort | Katsuhiko Nakahama |
| collection | DOAJ |
| description | Abstract Background Wood basic density (WBD), the biomass of plant cell walls per unit volume, is an important trait for elite tree selection in kraft pulp production. Here, we investigated the correlation between WBD and wood volumes or wood properties using 98 open-pollinated, 2.4 to 2.8 year-old hybrid Eucalyptus (Eucalyptus urophylla x E. grandis). Transcript levels of lignocellulose biosynthesis-related genes were studied. Results The progeny plants had average WBD of 516 kg/m3 with normal distribution and did not show any correlations between WBD and wood volume or components of α-cellulose, hemicellulose and Klason lignin content. Transcriptomic analysis of two groups of five plants each with high (570–609 kg/m3) or low (378–409 kg/m3) WBD was carried out by RNA-Seq analysis with total RNAs extracted from developing xylem tissues at a breast height. Lignocellulose biosynthesis-related genes, such as cellulose synthase, invertase, cinnamate-4-hydroxylase and cinnamoyl-CoA reductase showed higher transcript levels in the high WBD group. Among plant cell wall modifying genes, increased transcript levels of several expansin and xyloglucan endo-transglycosylase/hydrolase genes were also found in high WBD plants. Interestingly, strong transcript levels of several cytoskeleton genes encoding tubulin, actin and myosin were observed in high WBD plants. Furthermore, we also found elevated transcript levels of genes encoding NAC, MYB, basic helix-loop-helix, homeodomain, WRKY and LIM transcription factors in the high WBD plants. All these results indicate that the high WBD in plants has been associated with the increased transcription of many genes related to lignocellulose formation. Conclusions Most lignocellulose biosynthesis related genes exhibited a tendency to transcribe at relatively higher level in high WBD plants. These results suggest that lignocellulose biosynthesis-related genes may be associated with WBD. |
| first_indexed | 2024-12-20T06:45:30Z |
| format | Article |
| id | doaj.art-d33ae5548384421eb4b2e220a88b098a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T06:45:30Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-d33ae5548384421eb4b2e220a88b098a2022-12-21T19:49:44ZengBMCBMC Plant Biology1471-22292018-08-0118111210.1186/s12870-018-1371-9RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic densityKatsuhiko Nakahama0Nobuaki Urata1Tomotaka Shinya2Kazunori Hayashi3Kazuya Nanto4Antonio C. Rosa5Akiyoshi Kawaoka6Nippon Paper Industries Co., Ltd., Agri-Biotechnology Research LaboratoryNippon Paper Industries Co., Ltd., Agri-Biotechnology Research LaboratoryNippon Paper Industries Co., Ltd., Agri-Biotechnology Research LaboratoryForest Research Division, Amapá Florestal e Celulose S.ANippon Paper Industries Co., Ltd., Agri-Biotechnology Research LaboratoryForest Research Division, Amapá Florestal e Celulose S.ANippon Paper Industries Co., Ltd., Agri-Biotechnology Research LaboratoryAbstract Background Wood basic density (WBD), the biomass of plant cell walls per unit volume, is an important trait for elite tree selection in kraft pulp production. Here, we investigated the correlation between WBD and wood volumes or wood properties using 98 open-pollinated, 2.4 to 2.8 year-old hybrid Eucalyptus (Eucalyptus urophylla x E. grandis). Transcript levels of lignocellulose biosynthesis-related genes were studied. Results The progeny plants had average WBD of 516 kg/m3 with normal distribution and did not show any correlations between WBD and wood volume or components of α-cellulose, hemicellulose and Klason lignin content. Transcriptomic analysis of two groups of five plants each with high (570–609 kg/m3) or low (378–409 kg/m3) WBD was carried out by RNA-Seq analysis with total RNAs extracted from developing xylem tissues at a breast height. Lignocellulose biosynthesis-related genes, such as cellulose synthase, invertase, cinnamate-4-hydroxylase and cinnamoyl-CoA reductase showed higher transcript levels in the high WBD group. Among plant cell wall modifying genes, increased transcript levels of several expansin and xyloglucan endo-transglycosylase/hydrolase genes were also found in high WBD plants. Interestingly, strong transcript levels of several cytoskeleton genes encoding tubulin, actin and myosin were observed in high WBD plants. Furthermore, we also found elevated transcript levels of genes encoding NAC, MYB, basic helix-loop-helix, homeodomain, WRKY and LIM transcription factors in the high WBD plants. All these results indicate that the high WBD in plants has been associated with the increased transcription of many genes related to lignocellulose formation. Conclusions Most lignocellulose biosynthesis related genes exhibited a tendency to transcribe at relatively higher level in high WBD plants. These results suggest that lignocellulose biosynthesis-related genes may be associated with WBD.http://link.springer.com/article/10.1186/s12870-018-1371-9Hybrid EucalyptusWood basic densityCelluloseHemicelluloseLigninRNA-Seq |
| spellingShingle | Katsuhiko Nakahama Nobuaki Urata Tomotaka Shinya Kazunori Hayashi Kazuya Nanto Antonio C. Rosa Akiyoshi Kawaoka RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic density BMC Plant Biology Hybrid Eucalyptus Wood basic density Cellulose Hemicellulose Lignin RNA-Seq |
| title | RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic density |
| title_full | RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic density |
| title_fullStr | RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic density |
| title_full_unstemmed | RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic density |
| title_short | RNA-seq analysis of lignocellulose-related genes in hybrid Eucalyptus with contrasting wood basic density |
| title_sort | rna seq analysis of lignocellulose related genes in hybrid eucalyptus with contrasting wood basic density |
| topic | Hybrid Eucalyptus Wood basic density Cellulose Hemicellulose Lignin RNA-Seq |
| url | http://link.springer.com/article/10.1186/s12870-018-1371-9 |
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