Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific Activities
Pineapple weed <i>(Matricaria discoidea</i> DC.) is a widespread plant in Europe and North America. In ethnomedicine, it is well-known for its anti-inflammatory and spasmolytic activities. The aim of this research was to develop novel methods of <i>M. discoidea</i> processing...
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MDPI AG
2024-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/3/361 |
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| author | Janne Sepp Oleh Koshovyi Valdas Jakštas Vaidotas Žvikas Iryna Botsula Igor Kireyev Hanna Severina Oleksandr Kukhtenko Kaisa Põhako-Palu Karin Kogermann Jyrki Heinämäki Ain Raal |
| author_facet | Janne Sepp Oleh Koshovyi Valdas Jakštas Vaidotas Žvikas Iryna Botsula Igor Kireyev Hanna Severina Oleksandr Kukhtenko Kaisa Põhako-Palu Karin Kogermann Jyrki Heinämäki Ain Raal |
| author_sort | Janne Sepp |
| collection | DOAJ |
| description | Pineapple weed <i>(Matricaria discoidea</i> DC.) is a widespread plant in Europe and North America. In ethnomedicine, it is well-known for its anti-inflammatory and spasmolytic activities. The aim of this research was to develop novel methods of <i>M. discoidea</i> processing to obtain essential oil and dry extracts and to investigate their phytochemical compositions. Moreover, the molecular docking of the main substances and the in vivo studies on their soporific and analgesic activities were conducted. The essential oil and two dry extracts from <i>M. discoidea</i> were prepared. A total of 16 phenolic compounds (seven flavonoids, seven hydroxycinnamic acids, and two phenolic acids) in the dry extracts were identified by means of UPLC-MS/MS. In the essential oil, nine main terpenoids were identified by gas chromatography (GC). It was shown that phenolic extraction from the herb was successful when using 70% ethanol in a triple extraction method and at a ratio of 1:14–1:16. The in vivo studies with rodents demonstrated the analgesic activity of the <i>M. discoidea</i> extracts and improvements in the sleep of animals. The dry extracts of <i>M. discoidea</i> did not show any toxicity. The molecular docking analysis showed a high probability of COX-1,2 inhibition and NMDA receptor antagonism by the extracts. |
| first_indexed | 2024-04-24T18:30:36Z |
| format | Article |
| id | doaj.art-d3406a84470a4b0eb6b4a81c24e2ea3f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-24T18:30:36Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-d3406a84470a4b0eb6b4a81c24e2ea3f2024-03-27T13:28:04ZengMDPI AGBiomolecules2218-273X2024-03-0114336110.3390/biom14030361Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific ActivitiesJanne Sepp0Oleh Koshovyi1Valdas Jakštas2Vaidotas Žvikas3Iryna Botsula4Igor Kireyev5Hanna Severina6Oleksandr Kukhtenko7Kaisa Põhako-Palu8Karin Kogermann9Jyrki Heinämäki10Ain Raal11Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaInstitute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaDepartment of Clinical pharmacology and clinical pharmacy, National University of Pharmacy, 61002 Kharkiv, UkraineDepartment of Clinical pharmacology and clinical pharmacy, National University of Pharmacy, 61002 Kharkiv, UkrainePharmaceutical chemistry department, National University of Pharmacy, 61002 Kharkiv, UkrainePharmaceutical Technology of Drugs Department, National University of Pharmacy, 61002 Kharkiv, UkraineInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaInstitute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, EstoniaPineapple weed <i>(Matricaria discoidea</i> DC.) is a widespread plant in Europe and North America. In ethnomedicine, it is well-known for its anti-inflammatory and spasmolytic activities. The aim of this research was to develop novel methods of <i>M. discoidea</i> processing to obtain essential oil and dry extracts and to investigate their phytochemical compositions. Moreover, the molecular docking of the main substances and the in vivo studies on their soporific and analgesic activities were conducted. The essential oil and two dry extracts from <i>M. discoidea</i> were prepared. A total of 16 phenolic compounds (seven flavonoids, seven hydroxycinnamic acids, and two phenolic acids) in the dry extracts were identified by means of UPLC-MS/MS. In the essential oil, nine main terpenoids were identified by gas chromatography (GC). It was shown that phenolic extraction from the herb was successful when using 70% ethanol in a triple extraction method and at a ratio of 1:14–1:16. The in vivo studies with rodents demonstrated the analgesic activity of the <i>M. discoidea</i> extracts and improvements in the sleep of animals. The dry extracts of <i>M. discoidea</i> did not show any toxicity. The molecular docking analysis showed a high probability of COX-1,2 inhibition and NMDA receptor antagonism by the extracts.https://www.mdpi.com/2218-273X/14/3/361pineapple weedherbextractionterpenoidspolyphenolscytotoxicity |
| spellingShingle | Janne Sepp Oleh Koshovyi Valdas Jakštas Vaidotas Žvikas Iryna Botsula Igor Kireyev Hanna Severina Oleksandr Kukhtenko Kaisa Põhako-Palu Karin Kogermann Jyrki Heinämäki Ain Raal Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific Activities Biomolecules pineapple weed herb extraction terpenoids polyphenols cytotoxicity |
| title | Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific Activities |
| title_full | Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific Activities |
| title_fullStr | Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific Activities |
| title_full_unstemmed | Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific Activities |
| title_short | Phytochemical, Pharmacological, and Molecular Docking Study of Dry Extracts of <i>Matricaria discoidea</i> DC. with Analgesic and Soporific Activities |
| title_sort | phytochemical pharmacological and molecular docking study of dry extracts of i matricaria discoidea i dc with analgesic and soporific activities |
| topic | pineapple weed herb extraction terpenoids polyphenols cytotoxicity |
| url | https://www.mdpi.com/2218-273X/14/3/361 |
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