Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 Generation
The adverse effects of maternal pesticides exposure on the progeny is very well established. However, the impact of paternal exposure to pesticides such as Fenitrothion (FNT) on the histomorphometry of progeny’s organs in unexposed mothers are much less well studied. Therefore, this study aims to ev...
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MDPI AG
2021-07-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/9/7/159 |
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| author | Nur Afizah Yusoff Izatus Shima Taib Siti Balkis Budin Mahaneem Mohamed |
| author_facet | Nur Afizah Yusoff Izatus Shima Taib Siti Balkis Budin Mahaneem Mohamed |
| author_sort | Nur Afizah Yusoff |
| collection | DOAJ |
| description | The adverse effects of maternal pesticides exposure on the progeny is very well established. However, the impact of paternal exposure to pesticides such as Fenitrothion (FNT) on the histomorphometry of progeny’s organs in unexposed mothers are much less well studied. Therefore, this study aims to evaluate the effects of paternal FNT exposure on the sperm quality of the parent rat and its effects on the histomorphometry of the progeny’s organs. Randomly, male Sprague Dawley rats (<i>n</i> = 24) categorized as F0 were distributed equally into three groups namely Control, FNT-10, and FNT-20. Control received 1 mL/kg corn oil while FNT-10 and FNT-20 received 10 mg/kg and 20 mg/kg of FNT, respectively, via oral force feeding for 28 consecutive days. At the end of the study, male rats were mated with unexposed female rats and the male rats were sacrificed to obtain sperm for sperm characterization and DNA damage evaluation. Meanwhile, the rats’ progeny (F1) namely <i>p</i>Control, <i>p</i>FNT-10, and <i>p</i>FNT-20 were left to grow until postnatal day 70 before being sacrificed to obtain the matured organs for histology and morphometric analysis. Our results showed that both doses of FNT reduced sperm quality and caused DNA fragmentation in F0 rats compared with the control group (<i>p</i> < 0.05). The number of Leydig cells as well as the diameter of the seminiferous tubules and glomerulus of the <i>p</i>FNT-20 group had significantly decreased (<i>p</i> < 0.05) compared with the <i>p</i>Control group. The Bowman’s space of the <i>p</i>FNT-20 group had significantly increased (<i>p</i> < 0.05) compared with the <i>p</i>FNT-10 and <i>p</i>Control groups. Therefore, paternal exposure to FNT reduced the sperm quality and increased sperm DNA fragmentation in F0 male Sprague Dawley rats and altered the histology and morphometry of the selected organs in the F1 progeny. |
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| last_indexed | 2024-03-10T09:21:08Z |
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| spelling | doaj.art-d340a3f60eed4be0a38baff30bb9be552023-11-22T05:10:29ZengMDPI AGToxics2305-63042021-07-019715910.3390/toxics9070159Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 GenerationNur Afizah Yusoff0Izatus Shima Taib1Siti Balkis Budin2Mahaneem Mohamed3Biomedical Science Programme, Centre of Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaBiomedical Science Programme, Centre of Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaBiomedical Science Programme, Centre of Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, MalaysiaDepartment of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, MalaysiaThe adverse effects of maternal pesticides exposure on the progeny is very well established. However, the impact of paternal exposure to pesticides such as Fenitrothion (FNT) on the histomorphometry of progeny’s organs in unexposed mothers are much less well studied. Therefore, this study aims to evaluate the effects of paternal FNT exposure on the sperm quality of the parent rat and its effects on the histomorphometry of the progeny’s organs. Randomly, male Sprague Dawley rats (<i>n</i> = 24) categorized as F0 were distributed equally into three groups namely Control, FNT-10, and FNT-20. Control received 1 mL/kg corn oil while FNT-10 and FNT-20 received 10 mg/kg and 20 mg/kg of FNT, respectively, via oral force feeding for 28 consecutive days. At the end of the study, male rats were mated with unexposed female rats and the male rats were sacrificed to obtain sperm for sperm characterization and DNA damage evaluation. Meanwhile, the rats’ progeny (F1) namely <i>p</i>Control, <i>p</i>FNT-10, and <i>p</i>FNT-20 were left to grow until postnatal day 70 before being sacrificed to obtain the matured organs for histology and morphometric analysis. Our results showed that both doses of FNT reduced sperm quality and caused DNA fragmentation in F0 rats compared with the control group (<i>p</i> < 0.05). The number of Leydig cells as well as the diameter of the seminiferous tubules and glomerulus of the <i>p</i>FNT-20 group had significantly decreased (<i>p</i> < 0.05) compared with the <i>p</i>Control group. The Bowman’s space of the <i>p</i>FNT-20 group had significantly increased (<i>p</i> < 0.05) compared with the <i>p</i>FNT-10 and <i>p</i>Control groups. Therefore, paternal exposure to FNT reduced the sperm quality and increased sperm DNA fragmentation in F0 male Sprague Dawley rats and altered the histology and morphometry of the selected organs in the F1 progeny.https://www.mdpi.com/2305-6304/9/7/159histomorphometryinfertilityorganophosphateprogenyreproductive toxicitysperm DNA damage |
| spellingShingle | Nur Afizah Yusoff Izatus Shima Taib Siti Balkis Budin Mahaneem Mohamed Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 Generation Toxics histomorphometry infertility organophosphate progeny reproductive toxicity sperm DNA damage |
| title | Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 Generation |
| title_full | Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 Generation |
| title_fullStr | Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 Generation |
| title_full_unstemmed | Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 Generation |
| title_short | Paternal Fenitrothion Exposures in Rats Causes Sperm DNA Fragmentation in F0 and Histomorphometric Changes in Selected Organs of F1 Generation |
| title_sort | paternal fenitrothion exposures in rats causes sperm dna fragmentation in f0 and histomorphometric changes in selected organs of f1 generation |
| topic | histomorphometry infertility organophosphate progeny reproductive toxicity sperm DNA damage |
| url | https://www.mdpi.com/2305-6304/9/7/159 |
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