From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis
Abdominal sepsis triggers the transition of microorganisms from the gut to the peritoneum and bloodstream. Unfortunately, there is a limitation of methods and biomarkers to reliably study the emergence of pathobiomes and to monitor their respective dynamics. Three-month-old CD-1 female mice underwen...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/3/627 |
| _version_ | 1797436296946253824 |
|---|---|
| author | Christina Hartwig Susanne Drechsler Yevhen Vainshtein Madeline Maneth Theresa Schmitt Monika Ehling-Schulz Marcin Osuchowski Kai Sohn |
| author_facet | Christina Hartwig Susanne Drechsler Yevhen Vainshtein Madeline Maneth Theresa Schmitt Monika Ehling-Schulz Marcin Osuchowski Kai Sohn |
| author_sort | Christina Hartwig |
| collection | DOAJ |
| description | Abdominal sepsis triggers the transition of microorganisms from the gut to the peritoneum and bloodstream. Unfortunately, there is a limitation of methods and biomarkers to reliably study the emergence of pathobiomes and to monitor their respective dynamics. Three-month-old CD-1 female mice underwent cecal ligation and puncture (CLP) to induce abdominal sepsis. Serial and terminal endpoint specimens were collected for fecal, peritoneal lavage, and blood samples within 72 h. Microbial species compositions were determined by NGS of (cell-free) DNA and confirmed by microbiological cultivation. As a result, CLP induced rapid and early changes of gut microbial communities, with a transition of pathogenic species into the peritoneum and blood detected at 24 h post-CLP. NGS was able to identify pathogenic species in a time course-dependent manner in individual mice using cfDNA from as few as 30 microliters of blood. Absolute levels of cfDNA from pathogens changed rapidly during acute sepsis, demonstrating its short half-life. Pathogenic species and genera in CLP mice significantly overlapped with pathobiomes from septic patients. The study demonstrated that pathobiomes serve as reservoirs following CLP for the transition of pathogens into the bloodstream. Due to its short half-life, cfDNA can serve as a precise biomarker for pathogen identification in blood. |
| first_indexed | 2024-03-09T11:00:35Z |
| format | Article |
| id | doaj.art-d346c55ff0bc488eb91ccb1d9479fec0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:00:35Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-d346c55ff0bc488eb91ccb1d9479fec02023-12-01T01:21:54ZengMDPI AGMicroorganisms2076-26072023-02-0111362710.3390/microorganisms11030627From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine SepsisChristina Hartwig0Susanne Drechsler1Yevhen Vainshtein2Madeline Maneth3Theresa Schmitt4Monika Ehling-Schulz5Marcin Osuchowski6Kai Sohn7Innovation Field In-Vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, GermanyLudwig Boltzmann Institute for Traumatology the Research Centre in Cooperation with AUVA, 1200 Vienna, AustriaInnovation Field In-Vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, GermanyInnovation Field In-Vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, GermanyInnovation Field In-Vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, GermanyFunctional Microbiology, Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine Vienna, 1210 Vienna, AustriaLudwig Boltzmann Institute for Traumatology the Research Centre in Cooperation with AUVA, 1200 Vienna, AustriaInnovation Field In-Vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, 70569 Stuttgart, GermanyAbdominal sepsis triggers the transition of microorganisms from the gut to the peritoneum and bloodstream. Unfortunately, there is a limitation of methods and biomarkers to reliably study the emergence of pathobiomes and to monitor their respective dynamics. Three-month-old CD-1 female mice underwent cecal ligation and puncture (CLP) to induce abdominal sepsis. Serial and terminal endpoint specimens were collected for fecal, peritoneal lavage, and blood samples within 72 h. Microbial species compositions were determined by NGS of (cell-free) DNA and confirmed by microbiological cultivation. As a result, CLP induced rapid and early changes of gut microbial communities, with a transition of pathogenic species into the peritoneum and blood detected at 24 h post-CLP. NGS was able to identify pathogenic species in a time course-dependent manner in individual mice using cfDNA from as few as 30 microliters of blood. Absolute levels of cfDNA from pathogens changed rapidly during acute sepsis, demonstrating its short half-life. Pathogenic species and genera in CLP mice significantly overlapped with pathobiomes from septic patients. The study demonstrated that pathobiomes serve as reservoirs following CLP for the transition of pathogens into the bloodstream. Due to its short half-life, cfDNA can serve as a precise biomarker for pathogen identification in blood.https://www.mdpi.com/2076-2607/11/3/627cecal ligation punctureCLPgut microbiomecell-free DNApathogen liquid biopsysepsis |
| spellingShingle | Christina Hartwig Susanne Drechsler Yevhen Vainshtein Madeline Maneth Theresa Schmitt Monika Ehling-Schulz Marcin Osuchowski Kai Sohn From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis Microorganisms cecal ligation puncture CLP gut microbiome cell-free DNA pathogen liquid biopsy sepsis |
| title | From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis |
| title_full | From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis |
| title_fullStr | From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis |
| title_full_unstemmed | From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis |
| title_short | From Gut to Blood: Spatial and Temporal Pathobiome Dynamics during Acute Abdominal Murine Sepsis |
| title_sort | from gut to blood spatial and temporal pathobiome dynamics during acute abdominal murine sepsis |
| topic | cecal ligation puncture CLP gut microbiome cell-free DNA pathogen liquid biopsy sepsis |
| url | https://www.mdpi.com/2076-2607/11/3/627 |
| work_keys_str_mv | AT christinahartwig fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis AT susannedrechsler fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis AT yevhenvainshtein fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis AT madelinemaneth fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis AT theresaschmitt fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis AT monikaehlingschulz fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis AT marcinosuchowski fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis AT kaisohn fromguttobloodspatialandtemporalpathobiomedynamicsduringacuteabdominalmurinesepsis |