Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat
Wheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B × Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1147019/full |
| _version_ | 1811161653523251200 |
|---|---|
| author | Dengan Xu Qianlin Hao Tingzhi Yang Xinru Lv Huimin Qin Yalin Wang Chenfei Jia Wenxing Liu Xuehuan Dai Jianbin Zeng Hongsheng Zhang Zhonghu He Xianchun Xia Shuanghe Cao Wujun Ma |
| author_facet | Dengan Xu Qianlin Hao Tingzhi Yang Xinru Lv Huimin Qin Yalin Wang Chenfei Jia Wenxing Liu Xuehuan Dai Jianbin Zeng Hongsheng Zhang Zhonghu He Xianchun Xia Shuanghe Cao Wujun Ma |
| author_sort | Dengan Xu |
| collection | DOAJ |
| description | Wheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B × Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL mapping. Three QTL for CL were mapped on chromosomes 2BL, 4BS and 4DS. Of them, two major QTL QCL.qau-4BS and QCL.qau-4DS were detected, which could explain 9.1%–22.2% of the phenotypic variances across environments on Rht-B1 and Rht-D1 loci, respectively. Several studies have reported that Rht-B1b may reduce the length of wheat CL but no study has been carried out at molecular level. In order to verify that the Rht-B1 gene is the functional gene for the 4B QTL, an overexpression line Rht-B1b-OE and a CRISPR/SpCas9 line Rht-B1b-KO were studied. The results showed that Rht-B1b overexpression could reduce the CL, while loss-of-function of Rht-B1b would increase the CL relative to that of the null transgenic plants (TNL). To dissect the underlying regulatory mechanism of Rht-B1b on CL, comparative RNA-Seq was conducted between Rht-B1b-OE and TNL. Transcriptome profiles revealed a few key pathways involving the function of Rht-B1b in coleoptile development, including phytohormones, circadian rhythm and starch and sucrose metabolism. Our findings may facilitate wheat breeding for longer coleoptiles to improve seedling early vigor for better penetration through the soil crust in arid regions. |
| first_indexed | 2024-04-10T06:17:46Z |
| format | Article |
| id | doaj.art-d346ff1c2e624554a29d8db5bf17ad7f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T06:17:46Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d346ff1c2e624554a29d8db5bf17ad7f2023-03-02T05:47:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11470191147019Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheatDengan Xu0Qianlin Hao1Tingzhi Yang2Xinru Lv3Huimin Qin4Yalin Wang5Chenfei Jia6Wenxing Liu7Xuehuan Dai8Jianbin Zeng9Hongsheng Zhang10Zhonghu He11Xianchun Xia12Shuanghe Cao13Wujun Ma14College of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaNational Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Wheat Improvement Center, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao, ChinaWheat coleoptile is a sheath-like structure that helps to deliver the first leaf from embryo to the soil surface. Here, a RIL population consisting of 245 lines derived from Zhou 8425B × Chinese Spring cross was genotyped by the high-density Illumina iSelect 90K assay for coleoptile length (CL) QTL mapping. Three QTL for CL were mapped on chromosomes 2BL, 4BS and 4DS. Of them, two major QTL QCL.qau-4BS and QCL.qau-4DS were detected, which could explain 9.1%–22.2% of the phenotypic variances across environments on Rht-B1 and Rht-D1 loci, respectively. Several studies have reported that Rht-B1b may reduce the length of wheat CL but no study has been carried out at molecular level. In order to verify that the Rht-B1 gene is the functional gene for the 4B QTL, an overexpression line Rht-B1b-OE and a CRISPR/SpCas9 line Rht-B1b-KO were studied. The results showed that Rht-B1b overexpression could reduce the CL, while loss-of-function of Rht-B1b would increase the CL relative to that of the null transgenic plants (TNL). To dissect the underlying regulatory mechanism of Rht-B1b on CL, comparative RNA-Seq was conducted between Rht-B1b-OE and TNL. Transcriptome profiles revealed a few key pathways involving the function of Rht-B1b in coleoptile development, including phytohormones, circadian rhythm and starch and sucrose metabolism. Our findings may facilitate wheat breeding for longer coleoptiles to improve seedling early vigor for better penetration through the soil crust in arid regions.https://www.frontiersin.org/articles/10.3389/fpls.2023.1147019/fullwheatcoleoptile lengthRht-B1bQTLtranscriptome |
| spellingShingle | Dengan Xu Qianlin Hao Tingzhi Yang Xinru Lv Huimin Qin Yalin Wang Chenfei Jia Wenxing Liu Xuehuan Dai Jianbin Zeng Hongsheng Zhang Zhonghu He Xianchun Xia Shuanghe Cao Wujun Ma Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat Frontiers in Plant Science wheat coleoptile length Rht-B1b QTL transcriptome |
| title | Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat |
| title_full | Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat |
| title_fullStr | Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat |
| title_full_unstemmed | Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat |
| title_short | Impact of “Green Revolution” gene Rht-B1b on coleoptile length of wheat |
| title_sort | impact of green revolution gene rht b1b on coleoptile length of wheat |
| topic | wheat coleoptile length Rht-B1b QTL transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1147019/full |
| work_keys_str_mv | AT denganxu impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT qianlinhao impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT tingzhiyang impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT xinrulv impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT huiminqin impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT yalinwang impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT chenfeijia impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT wenxingliu impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT xuehuandai impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT jianbinzeng impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT hongshengzhang impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT zhonghuhe impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT xianchunxia impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT shuanghecao impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat AT wujunma impactofgreenrevolutiongenerhtb1boncoleoptilelengthofwheat |