Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton
Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequ...
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| Format: | Article |
| Language: | English |
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Universitas Udayana
2016-01-01
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| Series: | Jurnal Veteriner |
| Subjects: | |
| Online Access: | https://ojs.unud.ac.id/index.php/jvet/article/view/17340 |
| _version_ | 1818558632100364288 |
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| author | Asmarani Kusumawati Atik Ratnawati Ida Arlita Wulandari Sri Hartati Tri Untari |
| author_facet | Asmarani Kusumawati Atik Ratnawati Ida Arlita Wulandari Sri Hartati Tri Untari |
| author_sort | Asmarani Kusumawati |
| collection | DOAJ |
| description | Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA. |
| first_indexed | 2024-12-14T00:14:49Z |
| format | Article |
| id | doaj.art-d349ccb32a114c7d904a3622dcf57b46 |
| institution | Directory Open Access Journal |
| issn | 1411-8327 2477-5665 |
| language | English |
| last_indexed | 2024-12-14T00:14:49Z |
| publishDate | 2016-01-01 |
| publisher | Universitas Udayana |
| record_format | Article |
| series | Jurnal Veteriner |
| spelling | doaj.art-d349ccb32a114c7d904a3622dcf57b462022-12-21T23:25:35ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652016-01-0116456957517340Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based AmplificatonAsmarani Kusumawati0Atik RatnawatiIda Arlita WulandariSri HartatiTri UntariFakultas Kedokteran Hewan, Universitas Udayana, BaliJembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.https://ojs.unud.ac.id/index.php/jvet/article/view/17340Bali cattleNASBAenv-TM geneJDVTabanan 1995 strain |
| spellingShingle | Asmarani Kusumawati Atik Ratnawati Ida Arlita Wulandari Sri Hartati Tri Untari Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton Jurnal Veteriner Bali cattle NASBA env-TM gene JDV Tabanan 1995 strain |
| title | Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton |
| title_full | Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton |
| title_fullStr | Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton |
| title_full_unstemmed | Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton |
| title_short | Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton |
| title_sort | pelacakan gen env tm virus penyakit jembrana galur tabanan 1995 dengan metode nucleic acid sequence based amplificaton |
| topic | Bali cattle NASBA env-TM gene JDV Tabanan 1995 strain |
| url | https://ojs.unud.ac.id/index.php/jvet/article/view/17340 |
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