| Summary: | Alopecurus pratensis L. is one of the most important fodder grasses distributed in sub-frigid regions of the world. In this study, the complete chloroplast genome of A. pratensis was deciphered and is 136,157 bp in length. The genome includes a large single-copy region of 80,275 bp, small single-copy region of 12,830 bp, and a pair of inverted repeat regions of 21,526 bp. The GC content of the complete chloroplast genome of A. pratensis is 38.30%. Among the 134 unique genes in the circular genome, 38 tRNA, eight rRNA, and 88 protein-coding genes were annotated. We constructed the Maximum likelihood (ML) tree with 13 species from the Poaceae and found that A. pratensis was phylogenetically related to A. arundinaceus. The published A. pratensis chloroplast genome will provide useful information for phylogenetic and evolutionary study of the genus Alopecurus in the Poaceae.
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