Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in Mexico
Since Aromatic hydrocarbons are recalcitrant and toxic, strategies to remove them are needed. The aim of this work was to isolate fungi capable of using aromatic hydrocarbons as carbon sources. Two isolates from an oil polluted site in Mexico were identified through morphological and molecular marke...
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MDPI AG
2020-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/6/3/135 |
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| author | Martín R. Ide-Pérez Maikel Gilberto Fernández-López Ayixon Sánchez-Reyes Alfonso Leija Ramón Alberto Batista-García Jorge Luis Folch-Mallol María del Rayo Sánchez-Carbente |
| author_facet | Martín R. Ide-Pérez Maikel Gilberto Fernández-López Ayixon Sánchez-Reyes Alfonso Leija Ramón Alberto Batista-García Jorge Luis Folch-Mallol María del Rayo Sánchez-Carbente |
| author_sort | Martín R. Ide-Pérez |
| collection | DOAJ |
| description | Since Aromatic hydrocarbons are recalcitrant and toxic, strategies to remove them are needed. The aim of this work was to isolate fungi capable of using aromatic hydrocarbons as carbon sources. Two isolates from an oil polluted site in Mexico were identified through morphological and molecular markers as a novel <i>Rhodotorula</i> sp. and an <i>Exophiala</i> sp. Both strains were able to grow in a wide range of pH media, from 4 to 12, showing their optimal growth at alkaline pH’s and are both halotolerant. The <i>Exophiala</i> strain switched from hyphae to yeast morphotype in high salinity conditions. To the best of our knowledge, this is the first report of salt triggering dimorphism. The <i>Rhodotorula</i> strain, which is likely a new undescribed species, was capable of removing singled ringed aromatic compounds such as benzene, xylene, and toluene, but could not remove benzo[<i>a</i>] pyrene nor phenanthrene. Nevertheless, these hydrocarbons did not impair its growth. The <i>Exophiala</i> strain showed a different removal capacity. It could remove the polyaromatic hydrocarbons but performed poorly at removing toluene and xylene. Nevertheless, it still could grow well in the presence of the aromatic compounds. These strains could have a potential for aromatic compounds removal. |
| first_indexed | 2024-03-10T17:25:46Z |
| format | Article |
| id | doaj.art-d34c2317e35b4f528e962a0dc5a83a24 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T17:25:46Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-d34c2317e35b4f528e962a0dc5a83a242023-11-20T10:11:15ZengMDPI AGJournal of Fungi2309-608X2020-08-016313510.3390/jof6030135Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in MexicoMartín R. Ide-Pérez0Maikel Gilberto Fernández-López1Ayixon Sánchez-Reyes2Alfonso Leija3Ramón Alberto Batista-García4Jorge Luis Folch-Mallol5María del Rayo Sánchez-Carbente6Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, MexicoCentro de Investigación en Dinámica Celular-Instituto de Investigaciones Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, MexicoCátedras Conacyt-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62209, MexicoCentro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca 62209, MexicoCentro de Investigación en Dinámica Celular-Instituto de Investigaciones Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, MexicoCentro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, MexicoSince Aromatic hydrocarbons are recalcitrant and toxic, strategies to remove them are needed. The aim of this work was to isolate fungi capable of using aromatic hydrocarbons as carbon sources. Two isolates from an oil polluted site in Mexico were identified through morphological and molecular markers as a novel <i>Rhodotorula</i> sp. and an <i>Exophiala</i> sp. Both strains were able to grow in a wide range of pH media, from 4 to 12, showing their optimal growth at alkaline pH’s and are both halotolerant. The <i>Exophiala</i> strain switched from hyphae to yeast morphotype in high salinity conditions. To the best of our knowledge, this is the first report of salt triggering dimorphism. The <i>Rhodotorula</i> strain, which is likely a new undescribed species, was capable of removing singled ringed aromatic compounds such as benzene, xylene, and toluene, but could not remove benzo[<i>a</i>] pyrene nor phenanthrene. Nevertheless, these hydrocarbons did not impair its growth. The <i>Exophiala</i> strain showed a different removal capacity. It could remove the polyaromatic hydrocarbons but performed poorly at removing toluene and xylene. Nevertheless, it still could grow well in the presence of the aromatic compounds. These strains could have a potential for aromatic compounds removal.https://www.mdpi.com/2309-608X/6/3/135<i>Exophiala</i> sp.<i>Rhodotorula</i> sp.dimorphismaromatic hydrocarbonsphenanthrenebenzo[<i>a</i>]pyrene |
| spellingShingle | Martín R. Ide-Pérez Maikel Gilberto Fernández-López Ayixon Sánchez-Reyes Alfonso Leija Ramón Alberto Batista-García Jorge Luis Folch-Mallol María del Rayo Sánchez-Carbente Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in Mexico Journal of Fungi <i>Exophiala</i> sp. <i>Rhodotorula</i> sp. dimorphism aromatic hydrocarbons phenanthrene benzo[<i>a</i>]pyrene |
| title | Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in Mexico |
| title_full | Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in Mexico |
| title_fullStr | Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in Mexico |
| title_full_unstemmed | Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in Mexico |
| title_short | Aromatic Hydrocarbon Removal by Novel Extremotolerant <i>Exophiala</i> and <i>Rhodotorula</i> Spp. from an Oil Polluted Site in Mexico |
| title_sort | aromatic hydrocarbon removal by novel extremotolerant i exophiala i and i rhodotorula i spp from an oil polluted site in mexico |
| topic | <i>Exophiala</i> sp. <i>Rhodotorula</i> sp. dimorphism aromatic hydrocarbons phenanthrene benzo[<i>a</i>]pyrene |
| url | https://www.mdpi.com/2309-608X/6/3/135 |
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