Combinatorial pathway assembly in yeast
With the emergence of synthetic biology and the vast knowledge about individual biocatalytic reactions, the challenge nowadays is to implement whole natural or synthetic pathways into microorganisms. For this purpose balanced enzyme activities throughout the pathway need to be achieved in addition t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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AIMS Press
2015-10-01
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| Series: | AIMS Bioengineering |
| Subjects: | |
| Online Access: | http://www.aimspress.com/Bioengineering/article/473/fulltext.html |
| _version_ | 1811306156445925376 |
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| author | Khalil Essani Anton Glieder Martina Geier |
| author_facet | Khalil Essani Anton Glieder Martina Geier |
| author_sort | Khalil Essani |
| collection | DOAJ |
| description | With the emergence of synthetic biology and the vast knowledge about individual biocatalytic reactions, the challenge nowadays is to implement whole natural or synthetic pathways into microorganisms. For this purpose balanced enzyme activities throughout the pathway need to be achieved in addition to simple functional gene expression to avoid bottlenecks and to obtain high titers of the desired product. As the optimization of pathways in a specific biological context is often hard to achieve by rational design, combinatorial approaches have been developed to address this issue. Here, current strategies and proof of concepts for combinatorial pathway assembly in yeasts are reviewed. By exploiting its ability to join multiple DNA fragments in a very efficient and easy manner, the yeast <i>Saccharomyces cerevisiae</i> does not only constitute an attractive host for heterologous pathway expression, but also for assembling pathways by recombination <i>in vivo</i>. |
| first_indexed | 2024-04-13T08:39:22Z |
| format | Article |
| id | doaj.art-d34cf4e996824f0d943498d8d4de7314 |
| institution | Directory Open Access Journal |
| issn | 2375-1495 |
| language | English |
| last_indexed | 2024-04-13T08:39:22Z |
| publishDate | 2015-10-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Bioengineering |
| spelling | doaj.art-d34cf4e996824f0d943498d8d4de73142022-12-22T02:53:59ZengAIMS PressAIMS Bioengineering2375-14952015-10-012442343610.3934/bioeng.2015.4.423201504423Combinatorial pathway assembly in yeastKhalil EssaniAnton GliederMartina Geier0Austrian Centre of Industrial Biotechnology (ACIB GmbH), 8010 Graz, AustriaWith the emergence of synthetic biology and the vast knowledge about individual biocatalytic reactions, the challenge nowadays is to implement whole natural or synthetic pathways into microorganisms. For this purpose balanced enzyme activities throughout the pathway need to be achieved in addition to simple functional gene expression to avoid bottlenecks and to obtain high titers of the desired product. As the optimization of pathways in a specific biological context is often hard to achieve by rational design, combinatorial approaches have been developed to address this issue. Here, current strategies and proof of concepts for combinatorial pathway assembly in yeasts are reviewed. By exploiting its ability to join multiple DNA fragments in a very efficient and easy manner, the yeast <i>Saccharomyces cerevisiae</i> does not only constitute an attractive host for heterologous pathway expression, but also for assembling pathways by recombination <i>in vivo</i>.http://www.aimspress.com/Bioengineering/article/473/fulltext.htmlheterologous pathway expressionhomologous recombinationmetabolic engineering<i>Saccharomyces cerevisiae</i>synthetic biology |
| spellingShingle | Khalil Essani Anton Glieder Martina Geier Combinatorial pathway assembly in yeast AIMS Bioengineering heterologous pathway expression homologous recombination metabolic engineering <i>Saccharomyces cerevisiae</i> synthetic biology |
| title | Combinatorial pathway assembly in yeast |
| title_full | Combinatorial pathway assembly in yeast |
| title_fullStr | Combinatorial pathway assembly in yeast |
| title_full_unstemmed | Combinatorial pathway assembly in yeast |
| title_short | Combinatorial pathway assembly in yeast |
| title_sort | combinatorial pathway assembly in yeast |
| topic | heterologous pathway expression homologous recombination metabolic engineering <i>Saccharomyces cerevisiae</i> synthetic biology |
| url | http://www.aimspress.com/Bioengineering/article/473/fulltext.html |
| work_keys_str_mv | AT khalilessani combinatorialpathwayassemblyinyeast AT antonglieder combinatorialpathwayassemblyinyeast AT martinageier combinatorialpathwayassemblyinyeast |