Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae
Summary: Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed on olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that the ectopic expression of an AgOR (AgOR2) in ORNs dampened the activity of the expr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124722002273 |
| _version_ | 1828490474850091008 |
|---|---|
| author | Sarah E. Maguire Ali Afify Loyal A. Goff Christopher J. Potter |
| author_facet | Sarah E. Maguire Ali Afify Loyal A. Goff Christopher J. Potter |
| author_sort | Sarah E. Maguire |
| collection | DOAJ |
| description | Summary: Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed on olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that the ectopic expression of an AgOR (AgOR2) in ORNs dampened the activity of the expressing neuron. This contrasts with studies in Drosophila melanogaster in which the ectopic expression of non-native ORs in ORNs confers ectopic neuronal responses without interfering with native olfactory physiology. RNA-seq analyses comparing wild-type antennae to those ectopically expressing AgOR2 in ORNs indicated that nearly all AgOR transcripts were significantly downregulated (except for AgOR2). Additional experiments suggest that AgOR2 protein rather than mRNA mediates this downregulation. Using in situ hybridization, we find that AgOR gene choice is active into adulthood and that AgOR2 expression inhibits AgORs from turning on at this late stage. Our study shows that the ORNs of Anopheles mosquitoes (in contrast to Drosophila) are sensitive to a currently unexplored mechanism of AgOR regulation. |
| first_indexed | 2024-12-11T10:44:14Z |
| format | Article |
| id | doaj.art-d3534b33fa4448d4a761b77468a1976d |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T10:44:14Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-d3534b33fa4448d4a761b77468a1976d2022-12-22T01:10:31ZengElsevierCell Reports2211-12472022-03-013810110494Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiaeSarah E. Maguire0Ali Afify1Loyal A. Goff2Christopher J. Potter3Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Rangos 434, 855 N. Wolfe Street, Baltimore, MD 21205, USASolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Rangos 434, 855 N. Wolfe Street, Baltimore, MD 21205, USASolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Rangos 434, 855 N. Wolfe Street, Baltimore, MD 21205, USA; Department of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USASolomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Rangos 434, 855 N. Wolfe Street, Baltimore, MD 21205, USA; Corresponding authorSummary: Mosquitoes locate and approach humans based on the activity of odorant receptors (ORs) expressed on olfactory receptor neurons (ORNs). Olfactogenetic experiments in Anopheles gambiae mosquitoes revealed that the ectopic expression of an AgOR (AgOR2) in ORNs dampened the activity of the expressing neuron. This contrasts with studies in Drosophila melanogaster in which the ectopic expression of non-native ORs in ORNs confers ectopic neuronal responses without interfering with native olfactory physiology. RNA-seq analyses comparing wild-type antennae to those ectopically expressing AgOR2 in ORNs indicated that nearly all AgOR transcripts were significantly downregulated (except for AgOR2). Additional experiments suggest that AgOR2 protein rather than mRNA mediates this downregulation. Using in situ hybridization, we find that AgOR gene choice is active into adulthood and that AgOR2 expression inhibits AgORs from turning on at this late stage. Our study shows that the ORNs of Anopheles mosquitoes (in contrast to Drosophila) are sensitive to a currently unexplored mechanism of AgOR regulation.http://www.sciencedirect.com/science/article/pii/S2211124722002273Q-systemolfactioncalcium imagingsingle sensillum recordingsodorant receptorsRNA-seq |
| spellingShingle | Sarah E. Maguire Ali Afify Loyal A. Goff Christopher J. Potter Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae Cell Reports Q-system olfaction calcium imaging single sensillum recordings odorant receptors RNA-seq |
| title | Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae |
| title_full | Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae |
| title_fullStr | Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae |
| title_full_unstemmed | Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae |
| title_short | Odorant-receptor-mediated regulation of chemosensory gene expression in the malaria mosquito Anopheles gambiae |
| title_sort | odorant receptor mediated regulation of chemosensory gene expression in the malaria mosquito anopheles gambiae |
| topic | Q-system olfaction calcium imaging single sensillum recordings odorant receptors RNA-seq |
| url | http://www.sciencedirect.com/science/article/pii/S2211124722002273 |
| work_keys_str_mv | AT sarahemaguire odorantreceptormediatedregulationofchemosensorygeneexpressioninthemalariamosquitoanophelesgambiae AT aliafify odorantreceptormediatedregulationofchemosensorygeneexpressioninthemalariamosquitoanophelesgambiae AT loyalagoff odorantreceptormediatedregulationofchemosensorygeneexpressioninthemalariamosquitoanophelesgambiae AT christopherjpotter odorantreceptormediatedregulationofchemosensorygeneexpressioninthemalariamosquitoanophelesgambiae |