The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i>
Avian pathogenic <i>Escherichia coli</i> (APEC) is one of the common extraintestinal infectious disease pathogens in chickens, geese, and other birds. It can cause a variety of infections, and even the death of poultry, causing enormous economic losses. However, the misuse and abuse of a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/3/383 |
| _version_ | 1827760643290169344 |
|---|---|
| author | Kai Ma Hui Wang Zhenfei Lv Yutong Hu Hongli Wang Fang Shu Chengfeng Zhu Ting Xue |
| author_facet | Kai Ma Hui Wang Zhenfei Lv Yutong Hu Hongli Wang Fang Shu Chengfeng Zhu Ting Xue |
| author_sort | Kai Ma |
| collection | DOAJ |
| description | Avian pathogenic <i>Escherichia coli</i> (APEC) is one of the common extraintestinal infectious disease pathogens in chickens, geese, and other birds. It can cause a variety of infections, and even the death of poultry, causing enormous economic losses. However, the misuse and abuse of antibiotics in the poultry industry have led to the development of drug resistance in the gut microbes, posing a challenge for the treatment of APEC infections. It has been reported that the CpxRA two-component system has an effect on bacterial drug resistance, but the specific regulatory mechanism remains unclear. In this study, the regulatory mechanism of CpxRA on APEC biofilm formation and EmrKY efflux pump was investigated. The <i>cpxRA</i> knockout strain of <i>E. coli</i> APEC40 was constructed, and the molecular regulatory mechanism of CpxR on biofilms and efflux pump-coding genes were identified by biofilm formation assays, drug susceptibility test, real-time reverse transcription quantitative PCR, and electrophoretic mobility shift assay (EMSA). The results indicated that CpxR can directly bind to the promoter region of <i>emrKY</i> and negatively regulate the sensitivity of bacteria to ofloxacin and erythromycin. These results confirm the important regulatory role of the CpxRA two-component system under antibiotic stress in APEC. |
| first_indexed | 2024-03-11T09:54:26Z |
| format | Article |
| id | doaj.art-d356e394b3d64d759f6472155a8bb4b2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:54:26Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-d356e394b3d64d759f6472155a8bb4b22023-11-16T16:00:06ZengMDPI AGAnimals2076-26152023-01-0113338310.3390/ani13030383The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i>Kai Ma0Hui Wang1Zhenfei Lv2Yutong Hu3Hongli Wang4Fang Shu5Chengfeng Zhu6Ting Xue7School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaAvian pathogenic <i>Escherichia coli</i> (APEC) is one of the common extraintestinal infectious disease pathogens in chickens, geese, and other birds. It can cause a variety of infections, and even the death of poultry, causing enormous economic losses. However, the misuse and abuse of antibiotics in the poultry industry have led to the development of drug resistance in the gut microbes, posing a challenge for the treatment of APEC infections. It has been reported that the CpxRA two-component system has an effect on bacterial drug resistance, but the specific regulatory mechanism remains unclear. In this study, the regulatory mechanism of CpxRA on APEC biofilm formation and EmrKY efflux pump was investigated. The <i>cpxRA</i> knockout strain of <i>E. coli</i> APEC40 was constructed, and the molecular regulatory mechanism of CpxR on biofilms and efflux pump-coding genes were identified by biofilm formation assays, drug susceptibility test, real-time reverse transcription quantitative PCR, and electrophoretic mobility shift assay (EMSA). The results indicated that CpxR can directly bind to the promoter region of <i>emrKY</i> and negatively regulate the sensitivity of bacteria to ofloxacin and erythromycin. These results confirm the important regulatory role of the CpxRA two-component system under antibiotic stress in APEC.https://www.mdpi.com/2076-2615/13/3/383avian pathogenic <i>Escherichia coli</i>CpxRA two-component systemantibiotic susceptibilitybiofilms |
| spellingShingle | Kai Ma Hui Wang Zhenfei Lv Yutong Hu Hongli Wang Fang Shu Chengfeng Zhu Ting Xue The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i> Animals avian pathogenic <i>Escherichia coli</i> CpxRA two-component system antibiotic susceptibility biofilms |
| title | The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i> |
| title_full | The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i> |
| title_fullStr | The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i> |
| title_full_unstemmed | The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i> |
| title_short | The Two-Component System CpxRA Affects Antibiotic Susceptibility and Biofilm Formation in Avian Pathogenic <i>Escherichia coli</i> |
| title_sort | two component system cpxra affects antibiotic susceptibility and biofilm formation in avian pathogenic i escherichia coli i |
| topic | avian pathogenic <i>Escherichia coli</i> CpxRA two-component system antibiotic susceptibility biofilms |
| url | https://www.mdpi.com/2076-2615/13/3/383 |
| work_keys_str_mv | AT kaima thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT huiwang thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT zhenfeilv thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT yutonghu thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT hongliwang thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT fangshu thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT chengfengzhu thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT tingxue thetwocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT kaima twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT huiwang twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT zhenfeilv twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT yutonghu twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT hongliwang twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT fangshu twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT chengfengzhu twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii AT tingxue twocomponentsystemcpxraaffectsantibioticsusceptibilityandbiofilmformationinavianpathogeniciescherichiacolii |