Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and Bioactivity
Considering the increase in patients who suffer from osteoporosis and the bone defects that occur in these patients, bone tissue regeneration is a promising option to solve this problem. To achieve a synergistic effect between the synthesis of a proper structure and bioactive/pharmaceutical activity...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/1996-1944/17/2/373 |
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| author | Debora Carrozza Erika Ferrari Gianluca Malavasi |
| author_facet | Debora Carrozza Erika Ferrari Gianluca Malavasi |
| author_sort | Debora Carrozza |
| collection | DOAJ |
| description | Considering the increase in patients who suffer from osteoporosis and the bone defects that occur in these patients, bone tissue regeneration is a promising option to solve this problem. To achieve a synergistic effect between the synthesis of a proper structure and bioactive/pharmaceutical activity, ions with a physiological effect can be added to silica structures, such as Ca<sup>2+</sup>, thanks to its bioactive behavior, and Ga<sup>3+</sup> for its antibacterial and anticancer action. In this work, the synthesis of large pore mesoporous silica (LPMS), potential bioactive glasses containing Ca<sup>2+</sup> and Ga<sup>3+</sup>, has been studied. Corresponding structures, in terms of composition, have been synthesized following the Sol-Gel EISA (Evaporation Induced Self-Assembly) process (obtaining Classical Mesoporous Silica, MS). Pore structure characterization of LPMSs and MSs has been performed using N<sub>2</sub> adsorption/desorption and Hg-porosimetry, showing the presence of pores for LPMSs in the range of 20–60 and 200–600 nm. Nisin, a polycyclic antibacterial peptide, has been used for load tests. The load and release tests performed highlight a higher loading and releasing, doubled for LPMSs if compared to MSs. To confirm the maintenance of the structure of LPMSs and their mechanical strength and resistance, scanning electron microscopy images were acquired before and after release tests. Ca and Ga release in SBF has been studied through inductively coupled plasma—optical emission spectroscopy (ICP-OES), showing a particularly high release of these ions performed with LPMSs. The bioactive behavior of Ca-containing structures has been confirmed using FT-IR (Fourier-transform infrared spectroscopy), SEM-EDS (Scanning Electron Microscope-Energy Dispersive Spectroscopy), and X-ray powder diffraction (XRDP). In conclusion, LPMSs showed better loading and releasing properties compared with classical MS and better release in terms of active ions. In addition, it has also been demonstrated that LPMSs have bioactive behavior (a well-known characteristic of MSs). |
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| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-08T10:43:44Z |
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| series | Materials |
| spelling | doaj.art-d35cc49dff18457283dbcf85adc7a9ee2024-01-26T17:27:00ZengMDPI AGMaterials1996-19442024-01-0117237310.3390/ma17020373Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and BioactivityDebora Carrozza0Erika Ferrari1Gianluca Malavasi2Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, ItalyDepartment of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, ItalyDepartment of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, ItalyConsidering the increase in patients who suffer from osteoporosis and the bone defects that occur in these patients, bone tissue regeneration is a promising option to solve this problem. To achieve a synergistic effect between the synthesis of a proper structure and bioactive/pharmaceutical activity, ions with a physiological effect can be added to silica structures, such as Ca<sup>2+</sup>, thanks to its bioactive behavior, and Ga<sup>3+</sup> for its antibacterial and anticancer action. In this work, the synthesis of large pore mesoporous silica (LPMS), potential bioactive glasses containing Ca<sup>2+</sup> and Ga<sup>3+</sup>, has been studied. Corresponding structures, in terms of composition, have been synthesized following the Sol-Gel EISA (Evaporation Induced Self-Assembly) process (obtaining Classical Mesoporous Silica, MS). Pore structure characterization of LPMSs and MSs has been performed using N<sub>2</sub> adsorption/desorption and Hg-porosimetry, showing the presence of pores for LPMSs in the range of 20–60 and 200–600 nm. Nisin, a polycyclic antibacterial peptide, has been used for load tests. The load and release tests performed highlight a higher loading and releasing, doubled for LPMSs if compared to MSs. To confirm the maintenance of the structure of LPMSs and their mechanical strength and resistance, scanning electron microscopy images were acquired before and after release tests. Ca and Ga release in SBF has been studied through inductively coupled plasma—optical emission spectroscopy (ICP-OES), showing a particularly high release of these ions performed with LPMSs. The bioactive behavior of Ca-containing structures has been confirmed using FT-IR (Fourier-transform infrared spectroscopy), SEM-EDS (Scanning Electron Microscope-Energy Dispersive Spectroscopy), and X-ray powder diffraction (XRDP). In conclusion, LPMSs showed better loading and releasing properties compared with classical MS and better release in terms of active ions. In addition, it has also been demonstrated that LPMSs have bioactive behavior (a well-known characteristic of MSs).https://www.mdpi.com/1996-1944/17/2/373large pore mesoporous silicanisinbioactive glassesGa-containing glassespharmaceutical peptidesporous biomaterials |
| spellingShingle | Debora Carrozza Erika Ferrari Gianluca Malavasi Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and Bioactivity Materials large pore mesoporous silica nisin bioactive glasses Ga-containing glasses pharmaceutical peptides porous biomaterials |
| title | Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and Bioactivity |
| title_full | Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and Bioactivity |
| title_fullStr | Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and Bioactivity |
| title_full_unstemmed | Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and Bioactivity |
| title_short | Very Large Pore Mesoporous Bioactive Silicate Glasses: Comparison of Behavior toward Classical Mesoporous Bioactive Glasses in Terms of Drug Loading/Release and Bioactivity |
| title_sort | very large pore mesoporous bioactive silicate glasses comparison of behavior toward classical mesoporous bioactive glasses in terms of drug loading release and bioactivity |
| topic | large pore mesoporous silica nisin bioactive glasses Ga-containing glasses pharmaceutical peptides porous biomaterials |
| url | https://www.mdpi.com/1996-1944/17/2/373 |
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