Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection
The cervicovaginal microbiome (CVM) is a dynamic continuous microenvironment that can be clustered in microbial community state types (CSTs) and is associated with women’s cervical health. Lactobacillus-depleted communities particularly associate with an increased susceptibility for persistence of h...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037023003239 |
| _version_ | 1797384096714850304 |
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| author | Mariano A. Molina Willem J.G. Melchers Karolina M. Andralojc William P.J. Leenders Martijn A. Huynen |
| author_facet | Mariano A. Molina Willem J.G. Melchers Karolina M. Andralojc William P.J. Leenders Martijn A. Huynen |
| author_sort | Mariano A. Molina |
| collection | DOAJ |
| description | The cervicovaginal microbiome (CVM) is a dynamic continuous microenvironment that can be clustered in microbial community state types (CSTs) and is associated with women’s cervical health. Lactobacillus-depleted communities particularly associate with an increased susceptibility for persistence of high-risk human papillomavirus (hrHPV) infections and progression of disease, but the long-term ecological dynamics of CSTs after hrHPV infection diagnosis remain poorly understood. To determine such dynamics, we examined the CVM of our longitudinal cohort of 141 women diagnosed with hrHPV infection at baseline with collected cervical smears at two timepoints six-months apart. Here we describe that the long-term microbiome dissimilarity has a positive correlation with microbial diversity at both visits and that women with high abundance and dominance for Lactobacillus iners at baseline exhibit more similar microbiome composition at second visit than women with Lactobacillus-depleted communities at baseline. We further show that the species Lactobacillus acidophilus and Megasphaera genomosp type 1 associate with CST changes between both visits. Lastly, we also observe that Gardnerella vaginalis is associated with the stability of Lactobacillus-depleted communities while L. iners is associated with the instability of Megasphaera genomosp type 1-dominated communities. Our data suggest dynamic patterns of cervicovaginal CSTs during hrHPV infection, which could be potentially used to develop microbiome-based therapies against infection progression towards disease. |
| first_indexed | 2024-03-08T21:30:34Z |
| format | Article |
| id | doaj.art-d35db961d3b5445790e72f357751bb34 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-03-08T21:30:34Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-d35db961d3b5445790e72f357751bb342023-12-21T07:32:07ZengElsevierComputational and Structural Biotechnology Journal2001-03702023-01-012144244431Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infectionMariano A. Molina0Willem J.G. Melchers1Karolina M. Andralojc2William P.J. Leenders3Martijn A. Huynen4Department of Medical Microbiology, Radboud University Medical Center, 6500 HB, Nijmegen, the Netherlands; Department of Medical Microbiology, Radboud Institute for Molecular Life Sciences, Nijmegen, the NetherlandsDepartment of Medical Microbiology, Radboud University Medical Center, 6500 HB, Nijmegen, the NetherlandsDepartment of Medical Microbiology, Radboud University Medical Center, 6500 HB, Nijmegen, the NetherlandsPredica Diagnostics, Toernooiveld 1, 6525 ED, Nijmegen, the NetherlandsCenter for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, 6525 GA, Nijmegen, the Netherlands; Corresponding author.The cervicovaginal microbiome (CVM) is a dynamic continuous microenvironment that can be clustered in microbial community state types (CSTs) and is associated with women’s cervical health. Lactobacillus-depleted communities particularly associate with an increased susceptibility for persistence of high-risk human papillomavirus (hrHPV) infections and progression of disease, but the long-term ecological dynamics of CSTs after hrHPV infection diagnosis remain poorly understood. To determine such dynamics, we examined the CVM of our longitudinal cohort of 141 women diagnosed with hrHPV infection at baseline with collected cervical smears at two timepoints six-months apart. Here we describe that the long-term microbiome dissimilarity has a positive correlation with microbial diversity at both visits and that women with high abundance and dominance for Lactobacillus iners at baseline exhibit more similar microbiome composition at second visit than women with Lactobacillus-depleted communities at baseline. We further show that the species Lactobacillus acidophilus and Megasphaera genomosp type 1 associate with CST changes between both visits. Lastly, we also observe that Gardnerella vaginalis is associated with the stability of Lactobacillus-depleted communities while L. iners is associated with the instability of Megasphaera genomosp type 1-dominated communities. Our data suggest dynamic patterns of cervicovaginal CSTs during hrHPV infection, which could be potentially used to develop microbiome-based therapies against infection progression towards disease.http://www.sciencedirect.com/science/article/pii/S2001037023003239MicrobiomeDynamicsMicrobial communitiesHrHPVCervical cancerCervicovaginal microbiome |
| spellingShingle | Mariano A. Molina Willem J.G. Melchers Karolina M. Andralojc William P.J. Leenders Martijn A. Huynen Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection Computational and Structural Biotechnology Journal Microbiome Dynamics Microbial communities HrHPV Cervical cancer Cervicovaginal microbiome |
| title | Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection |
| title_full | Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection |
| title_fullStr | Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection |
| title_full_unstemmed | Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection |
| title_short | Longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrHPV infection |
| title_sort | longitudinal analysis on the ecological dynamics of the cervicovaginal microbiome in hrhpv infection |
| topic | Microbiome Dynamics Microbial communities HrHPV Cervical cancer Cervicovaginal microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S2001037023003239 |
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