Metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms
Abstract Background Ginseng rusty root symptoms (GRS) is one of the primary diseases of ginseng. This disease leads to a severe decline in the quality of ginseng. It has been shown that the occurrence of GRS is associated with soil environmental degradation, which may involve changes in soil microbi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-05-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-021-03001-w |
| _version_ | 1818453799919943680 |
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| author | Xingbo Bian Yan Zhao Shengyuan Xiao He Yang Yongzhong Han Lianxue Zhang |
| author_facet | Xingbo Bian Yan Zhao Shengyuan Xiao He Yang Yongzhong Han Lianxue Zhang |
| author_sort | Xingbo Bian |
| collection | DOAJ |
| description | Abstract Background Ginseng rusty root symptoms (GRS) is one of the primary diseases of ginseng. This disease leads to a severe decline in the quality of ginseng. It has been shown that the occurrence of GRS is associated with soil environmental degradation, which may involve changes in soil microbiology and physicochemical properties. Results In this study, GRS and healthy ginseng (HG) samples were used as experimental materials for comparative analysis of transcriptome and metabolome. Compared with those in HG samples, 949 metabolites and 9451 genes were significantly changed at the metabolic and transcriptional levels in diseased samples. The diseased tissues’ metabolic patterns changed, and the accumulation of various organic acids, alkaloids, alcohols and phenols in diseased tissues increased significantly. There were significant differences in the expression of genes involved in plant hormone signal transduction, phenylpropanoid biosynthesis, the peroxidase pathway, and the plant-pathogen interaction pathway. Conclusion The current study involved a comparative metabolome and transcriptome analysis of GRS and HG samples. Based on the findings at the transcriptional and metabolic levels, a mechanism model of the ginseng response to GRS was established. Our results provide new insights into ginseng’s response to GRS, which will reveal the potential molecular mechanisms of this disease in ginseng. |
| first_indexed | 2024-12-14T21:44:44Z |
| format | Article |
| id | doaj.art-d35e5502741a4ffc9a2bb52c54ac9e9c |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-14T21:44:44Z |
| publishDate | 2021-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-d35e5502741a4ffc9a2bb52c54ac9e9c2022-12-21T22:46:23ZengBMCBMC Plant Biology1471-22292021-05-0121111610.1186/s12870-021-03001-wMetabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptomsXingbo Bian0Yan Zhao1Shengyuan Xiao2He Yang3Yongzhong Han4Lianxue Zhang5College of Chinese Medicinal Materials, Jilin Agricultural UniversityCollege of Chinese Medicinal Materials, Jilin Agricultural UniversityCollege of Chinese Medicinal Materials, Jilin Agricultural UniversityCollege of Chinese Medicinal Materials, Jilin Agricultural UniversityJilin Provincial Ginseng and Pilose Antler OfficeCollege of Chinese Medicinal Materials, Jilin Agricultural UniversityAbstract Background Ginseng rusty root symptoms (GRS) is one of the primary diseases of ginseng. This disease leads to a severe decline in the quality of ginseng. It has been shown that the occurrence of GRS is associated with soil environmental degradation, which may involve changes in soil microbiology and physicochemical properties. Results In this study, GRS and healthy ginseng (HG) samples were used as experimental materials for comparative analysis of transcriptome and metabolome. Compared with those in HG samples, 949 metabolites and 9451 genes were significantly changed at the metabolic and transcriptional levels in diseased samples. The diseased tissues’ metabolic patterns changed, and the accumulation of various organic acids, alkaloids, alcohols and phenols in diseased tissues increased significantly. There were significant differences in the expression of genes involved in plant hormone signal transduction, phenylpropanoid biosynthesis, the peroxidase pathway, and the plant-pathogen interaction pathway. Conclusion The current study involved a comparative metabolome and transcriptome analysis of GRS and HG samples. Based on the findings at the transcriptional and metabolic levels, a mechanism model of the ginseng response to GRS was established. Our results provide new insights into ginseng’s response to GRS, which will reveal the potential molecular mechanisms of this disease in ginseng.https://doi.org/10.1186/s12870-021-03001-wPanax ginsengRusty rootMetabolomeTranscriptomeEnvironmental stress |
| spellingShingle | Xingbo Bian Yan Zhao Shengyuan Xiao He Yang Yongzhong Han Lianxue Zhang Metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms BMC Plant Biology Panax ginseng Rusty root Metabolome Transcriptome Environmental stress |
| title | Metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms |
| title_full | Metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms |
| title_fullStr | Metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms |
| title_full_unstemmed | Metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms |
| title_short | Metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms |
| title_sort | metabolome and transcriptome analysis reveals the molecular profiles underlying the ginseng response to rusty root symptoms |
| topic | Panax ginseng Rusty root Metabolome Transcriptome Environmental stress |
| url | https://doi.org/10.1186/s12870-021-03001-w |
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