Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in Japan
Abstract Background Salmonella contamination of raw meat‐based diets (RMBDs) for pets poses a major public health concern but has not been investigated in Japan. Objective To investigate Salmonella contamination in RMBDs for dogs marketed in Japan and the anti‐microbial resistance profiles of the Sa...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.739 |
| _version_ | 1827816440042881024 |
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| author | Shoichiro Yukawa Ikuo Uchida Hiroshi Takemitsu Asako Okamoto Motomi Yukawa Seinosuke Ohshima Yutaka Tamura |
| author_facet | Shoichiro Yukawa Ikuo Uchida Hiroshi Takemitsu Asako Okamoto Motomi Yukawa Seinosuke Ohshima Yutaka Tamura |
| author_sort | Shoichiro Yukawa |
| collection | DOAJ |
| description | Abstract Background Salmonella contamination of raw meat‐based diets (RMBDs) for pets poses a major public health concern but has not been investigated in Japan. Objective To investigate Salmonella contamination in RMBDs for dogs marketed in Japan and the anti‐microbial resistance profiles of the Salmonella isolates. Methods Sixty commercial RMBD samples were collected in the Okayama and Osaka Prefectures, Japan, between December 2016 and March 2017. The obtained Salmonella isolates were serotyped, their anti‐microbial resistance patterns were determined, and the anti‐microbial‐resistant isolates were screened for the presence of resistance genes by polymerase chain reaction. Results Salmonella enterica subsp. enterica was detected in seven of the 60 RMBD samples. Among them, five isolates were identified as S. Infantis (n = 3), S. Typhimurium (n = 1) and S. Schwarzengrund (n = 1), while the serotypes of two isolates were unable to be identified. All isolates were susceptible to ampicillin, cefazolin, cefotaxime and gentamycin. Two isolates were resistant to more than one anti‐microbial agent; one of the S. Infantis isolates was resistant to streptomycin, kanamycin, tetracycline and trimethoprim, while the S. Typhimurium isolate was resistant to nalidixic acid, ciprofloxacin and chloramphenicol. The S. Schwarzengrund isolate was resistant to tetracycline. Additionally, the S. Typhimurium isolate harboured the anti‐microbial resistance gene gyrA with a mutation corresponding to Ser‐83→Phe amino acid substitution. Conclusion The study findings suggest that RMBDs for dogs marketed in Japan can be a potential source of Salmonella infection for dogs and humans including infections caused by quinolone‐resistant isolates. |
| first_indexed | 2024-03-12T00:15:21Z |
| format | Article |
| id | doaj.art-d35ebd96cce146a19b1aa9ca8f83c578 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-12T00:15:21Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-d35ebd96cce146a19b1aa9ca8f83c5782023-09-15T15:20:44ZengWileyVeterinary Medicine and Science2053-10952022-05-018398298910.1002/vms3.739Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in JapanShoichiro Yukawa0Ikuo Uchida1Hiroshi Takemitsu2Asako Okamoto3Motomi Yukawa4Seinosuke Ohshima5Yutaka Tamura6Department of Comparative Animal Science, College of Life Science Kurashiki University of Science and The Arts Kurashiki‐shi Okayama JapanDepartment of Pathobiology, School of Veterinary Medicine Rakuno Gakuen University Ebetsu‐shi Hokkaido JapanDepartment of Comparative Animal Science, College of Life Science Kurashiki University of Science and The Arts Kurashiki‐shi Okayama JapanDepartment of Comparative Animal Science, College of Life Science Kurashiki University of Science and The Arts Kurashiki‐shi Okayama JapanYukawa Animal Clinic Kainan‐shi Wakayama JapanDepartment of Comparative Animal Science, College of Life Science Kurashiki University of Science and The Arts Kurashiki‐shi Okayama JapanCenter for Veterinary Drug Development Rakuno Gakuen University Ebetsu‐shi Hokkaido JapanAbstract Background Salmonella contamination of raw meat‐based diets (RMBDs) for pets poses a major public health concern but has not been investigated in Japan. Objective To investigate Salmonella contamination in RMBDs for dogs marketed in Japan and the anti‐microbial resistance profiles of the Salmonella isolates. Methods Sixty commercial RMBD samples were collected in the Okayama and Osaka Prefectures, Japan, between December 2016 and March 2017. The obtained Salmonella isolates were serotyped, their anti‐microbial resistance patterns were determined, and the anti‐microbial‐resistant isolates were screened for the presence of resistance genes by polymerase chain reaction. Results Salmonella enterica subsp. enterica was detected in seven of the 60 RMBD samples. Among them, five isolates were identified as S. Infantis (n = 3), S. Typhimurium (n = 1) and S. Schwarzengrund (n = 1), while the serotypes of two isolates were unable to be identified. All isolates were susceptible to ampicillin, cefazolin, cefotaxime and gentamycin. Two isolates were resistant to more than one anti‐microbial agent; one of the S. Infantis isolates was resistant to streptomycin, kanamycin, tetracycline and trimethoprim, while the S. Typhimurium isolate was resistant to nalidixic acid, ciprofloxacin and chloramphenicol. The S. Schwarzengrund isolate was resistant to tetracycline. Additionally, the S. Typhimurium isolate harboured the anti‐microbial resistance gene gyrA with a mutation corresponding to Ser‐83→Phe amino acid substitution. Conclusion The study findings suggest that RMBDs for dogs marketed in Japan can be a potential source of Salmonella infection for dogs and humans including infections caused by quinolone‐resistant isolates.https://doi.org/10.1002/vms3.739dog foodraw meat‐based diet, Salmonella |
| spellingShingle | Shoichiro Yukawa Ikuo Uchida Hiroshi Takemitsu Asako Okamoto Motomi Yukawa Seinosuke Ohshima Yutaka Tamura Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in Japan Veterinary Medicine and Science dog food raw meat‐based diet, Salmonella |
| title | Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in Japan |
| title_full | Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in Japan |
| title_fullStr | Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in Japan |
| title_full_unstemmed | Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in Japan |
| title_short | Anti‐microbial resistance of Salmonella isolates from raw meat‐based dog food in Japan |
| title_sort | anti microbial resistance of salmonella isolates from raw meat based dog food in japan |
| topic | dog food raw meat‐based diet, Salmonella |
| url | https://doi.org/10.1002/vms3.739 |
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