Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress
Thiocyanate (SCN−) can find its way into cultivated fields, which might hamper the harmony in carbon and nitrogen metabolism (CNM) of plants, ebbing their quality and productivity. In the current study, we investigated the role of the exogenous application of 2-oxoglutarate (2-OG) in maintaining hom...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1086098/full |
| _version_ | 1797897105122000896 |
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| author | Yu-Xi Feng Li Yang Yu-Juan Lin Ying Song Xiao-Zhang Yu |
| author_facet | Yu-Xi Feng Li Yang Yu-Juan Lin Ying Song Xiao-Zhang Yu |
| author_sort | Yu-Xi Feng |
| collection | DOAJ |
| description | Thiocyanate (SCN−) can find its way into cultivated fields, which might hamper the harmony in carbon and nitrogen metabolism (CNM) of plants, ebbing their quality and productivity. In the current study, we investigated the role of the exogenous application of 2-oxoglutarate (2-OG) in maintaining homeostasis of CNM in rice seedlings under SCN− stress. Results showed that SCN− exposure significantly repressed the gene expression and activities of CNM-related enzymes (e.g., phosphoenolpyruvate carboxylase, NADP-dependent isocitrate dehydrogenases, and isocitrate dehydrogenases) in rice seedlings, thereby reducing their relative growth rate (RGR). Exogenous application of 2-OG effectively mitigated the toxic effects of SCN− on rice seedlings, judged by the aforementioned parameters. The co-expression network analysis showed that genes activated in CNM pathways were categorized into four modules (Modules 1–4). In order to identify the key module activated in CNM in rice seedlings exposed to SCN−, the results from real-time quantitative PCR (RT-qPCR) tests were used to calculate the possibility of the occurrence of genes grouped in four different modules. Notably, Module 3 showed the highest occurrence probability, which is mainly related to N metabolism and 2-OG synthesis. We can conclude that exogenous application of 2-OG can modify the imbalance of CNM caused by SCN− exposure through regulating N metabolism and 2-OG synthesis in rice seedlings. |
| first_indexed | 2024-04-10T07:53:33Z |
| format | Article |
| id | doaj.art-d3606ad03b9e488c85a66863f0de85bf |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T07:53:33Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d3606ad03b9e488c85a66863f0de85bf2023-02-23T07:54:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.10860981086098Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stressYu-Xi FengLi YangYu-Juan LinYing SongXiao-Zhang YuThiocyanate (SCN−) can find its way into cultivated fields, which might hamper the harmony in carbon and nitrogen metabolism (CNM) of plants, ebbing their quality and productivity. In the current study, we investigated the role of the exogenous application of 2-oxoglutarate (2-OG) in maintaining homeostasis of CNM in rice seedlings under SCN− stress. Results showed that SCN− exposure significantly repressed the gene expression and activities of CNM-related enzymes (e.g., phosphoenolpyruvate carboxylase, NADP-dependent isocitrate dehydrogenases, and isocitrate dehydrogenases) in rice seedlings, thereby reducing their relative growth rate (RGR). Exogenous application of 2-OG effectively mitigated the toxic effects of SCN− on rice seedlings, judged by the aforementioned parameters. The co-expression network analysis showed that genes activated in CNM pathways were categorized into four modules (Modules 1–4). In order to identify the key module activated in CNM in rice seedlings exposed to SCN−, the results from real-time quantitative PCR (RT-qPCR) tests were used to calculate the possibility of the occurrence of genes grouped in four different modules. Notably, Module 3 showed the highest occurrence probability, which is mainly related to N metabolism and 2-OG synthesis. We can conclude that exogenous application of 2-OG can modify the imbalance of CNM caused by SCN− exposure through regulating N metabolism and 2-OG synthesis in rice seedlings.https://www.frontiersin.org/articles/10.3389/fpls.2023.1086098/fullricethiocyanate2-OGcarbon metabolismnitrogen metabolism |
| spellingShingle | Yu-Xi Feng Li Yang Yu-Juan Lin Ying Song Xiao-Zhang Yu Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress Frontiers in Plant Science rice thiocyanate 2-OG carbon metabolism nitrogen metabolism |
| title | Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress |
| title_full | Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress |
| title_fullStr | Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress |
| title_full_unstemmed | Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress |
| title_short | Merging the occurrence possibility into gene co-expression network deciphers the importance of exogenous 2-oxoglutarate in improving the growth of rice seedlings under thiocyanate stress |
| title_sort | merging the occurrence possibility into gene co expression network deciphers the importance of exogenous 2 oxoglutarate in improving the growth of rice seedlings under thiocyanate stress |
| topic | rice thiocyanate 2-OG carbon metabolism nitrogen metabolism |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1086098/full |
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