Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i>
The rice leaf folder, <i>Cnaphalocrocis medinalis</i> (Lepidoptera: Pyralidae), is a notorious pest of rice in Asia. The larvae and adults of <i>C. medinalis</i> utilize specialized chemosensory systems to adapt to different environmental odors and physiological behaviors. Ho...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/12/2165 |
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| author | Hai-Tao Du Jia-Qi Lu Kun Ji Chu-Chu Wang Zhi-Chao Yao Fang Liu Yao Li |
| author_facet | Hai-Tao Du Jia-Qi Lu Kun Ji Chu-Chu Wang Zhi-Chao Yao Fang Liu Yao Li |
| author_sort | Hai-Tao Du |
| collection | DOAJ |
| description | The rice leaf folder, <i>Cnaphalocrocis medinalis</i> (Lepidoptera: Pyralidae), is a notorious pest of rice in Asia. The larvae and adults of <i>C. medinalis</i> utilize specialized chemosensory systems to adapt to different environmental odors and physiological behaviors. However, the differences in chemosensory genes between the olfactory organs of these two different developmental stages remain unclear. Here, we conducted a transcriptome analysis of larvae heads, male antennae, and female antennae in <i>C. medinalis</i> and identified 131 putative chemosensory genes, including 32 <i>OBP</i>s (8 novel <i>OBP</i>s), 23 <i>CSP</i>s (2 novel <i>CSP</i>s), 55 <i>OR</i>s (17 novel <i>OR</i>s), 19 <i>IR</i>s (5 novel <i>IR</i>s) and 2 <i>SNMP</i>s. Comparisons between larvae and adults of <i>C. medinalis</i> by transcriptome and RT-qPCR analysis revealed that the number and expression of chemosensory genes in larval heads were less than that of adult antennae. Only 17 chemosensory genes (7 <i>OBP</i>s and 10 <i>CSP</i>s) were specifically or preferentially expressed in the larval heads, while a total of 101 chemosensory genes (21 <i>OBP</i>s, 9 <i>CSP</i>s, 51 <i>OR</i>s, 18 <i>IR</i>s, and 2 <i>SNMP</i>s) were specifically or preferentially expressed in adult antennae. Our study found differences in chemosensory gene expression between larvae and adults, suggesting their specialized functions at different developmental stages of <i>C. medinalis</i>. These results provide a theoretical basis for screening chemosensory genes as potential molecular targets and developing novel management strategies to control <i>C. medinalis</i>. |
| first_indexed | 2024-03-08T20:45:07Z |
| format | Article |
| id | doaj.art-d361888d61df4636a7d0990a125402b2 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-08T20:45:07Z |
| publishDate | 2023-11-01 |
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| series | Genes |
| spelling | doaj.art-d361888d61df4636a7d0990a125402b22023-12-22T14:11:09ZengMDPI AGGenes2073-44252023-11-011412216510.3390/genes14122165Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i>Hai-Tao Du0Jia-Qi Lu1Kun Ji2Chu-Chu Wang3Zhi-Chao Yao4Fang Liu5Yao Li6College of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaCollege of Plant Protection, Yangzhou University, Yangzhou 225009, ChinaThe rice leaf folder, <i>Cnaphalocrocis medinalis</i> (Lepidoptera: Pyralidae), is a notorious pest of rice in Asia. The larvae and adults of <i>C. medinalis</i> utilize specialized chemosensory systems to adapt to different environmental odors and physiological behaviors. However, the differences in chemosensory genes between the olfactory organs of these two different developmental stages remain unclear. Here, we conducted a transcriptome analysis of larvae heads, male antennae, and female antennae in <i>C. medinalis</i> and identified 131 putative chemosensory genes, including 32 <i>OBP</i>s (8 novel <i>OBP</i>s), 23 <i>CSP</i>s (2 novel <i>CSP</i>s), 55 <i>OR</i>s (17 novel <i>OR</i>s), 19 <i>IR</i>s (5 novel <i>IR</i>s) and 2 <i>SNMP</i>s. Comparisons between larvae and adults of <i>C. medinalis</i> by transcriptome and RT-qPCR analysis revealed that the number and expression of chemosensory genes in larval heads were less than that of adult antennae. Only 17 chemosensory genes (7 <i>OBP</i>s and 10 <i>CSP</i>s) were specifically or preferentially expressed in the larval heads, while a total of 101 chemosensory genes (21 <i>OBP</i>s, 9 <i>CSP</i>s, 51 <i>OR</i>s, 18 <i>IR</i>s, and 2 <i>SNMP</i>s) were specifically or preferentially expressed in adult antennae. Our study found differences in chemosensory gene expression between larvae and adults, suggesting their specialized functions at different developmental stages of <i>C. medinalis</i>. These results provide a theoretical basis for screening chemosensory genes as potential molecular targets and developing novel management strategies to control <i>C. medinalis</i>.https://www.mdpi.com/2073-4425/14/12/2165<i>Cnaphalocrocis medinalis</i>chemosensory geneslarvaadulttranscriptome analysisexpression pattern |
| spellingShingle | Hai-Tao Du Jia-Qi Lu Kun Ji Chu-Chu Wang Zhi-Chao Yao Fang Liu Yao Li Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i> Genes <i>Cnaphalocrocis medinalis</i> chemosensory genes larva adult transcriptome analysis expression pattern |
| title | Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i> |
| title_full | Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i> |
| title_fullStr | Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i> |
| title_full_unstemmed | Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i> |
| title_short | Comparative Transcriptomic Assessment of Chemosensory Genes in Adult and Larval Olfactory Organs of <i>Cnaphalocrocis medinalis</i> |
| title_sort | comparative transcriptomic assessment of chemosensory genes in adult and larval olfactory organs of i cnaphalocrocis medinalis i |
| topic | <i>Cnaphalocrocis medinalis</i> chemosensory genes larva adult transcriptome analysis expression pattern |
| url | https://www.mdpi.com/2073-4425/14/12/2165 |
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