Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program.
Rapid advances in biological and digital support systems are revolutionizing the population control of invasive disease vectors such as Aedes aegypti. Methods such as the sterile and incompatible insect techniques (SIT/IIT) rely on modified males to seek out and successfully mate with females, and i...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-06-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0009357 |
| _version_ | 1818567956005650432 |
|---|---|
| author | Brendan J Trewin Daniel E Pagendam Brian J Johnson Chris Paton Nigel Snoad Scott A Ritchie Kyran M Staunton Bradley J White Sara Mitchell Nigel W Beebe |
| author_facet | Brendan J Trewin Daniel E Pagendam Brian J Johnson Chris Paton Nigel Snoad Scott A Ritchie Kyran M Staunton Bradley J White Sara Mitchell Nigel W Beebe |
| author_sort | Brendan J Trewin |
| collection | DOAJ |
| description | Rapid advances in biological and digital support systems are revolutionizing the population control of invasive disease vectors such as Aedes aegypti. Methods such as the sterile and incompatible insect techniques (SIT/IIT) rely on modified males to seek out and successfully mate with females, and in doing so outcompete the wild male population for mates. Currently, these interventions most frequently infer mating success through area-wide population surveillance and estimates of mating competitiveness are rare. Furthermore, little is known about male Ae. aegypti behaviour and biology in field settings. In preparation for a large, community scale IIT program, we undertook a series of mark- release-recapture experiments using rhodamine B to mark male Ae. aegypti sperm and measure mating interactions with females. We also developed a Spatial and Temporally Evolving Isotropic Kernel (STEIK) framework to assist researchers to estimate the movement of individuals through space and time. Results showed that ~40% of wild females captured daily were unmated, suggesting interventions will need to release males multiple times per week to be effective at suppressing Ae. aegypti populations. Males moved rapidly through the landscape, particularly when released during the night. Although males moved further than what is typically observed in females of the species, survival was considerably lower. These unique insights improve our understanding of mating interactions in wild Ae. aegypti populations and lay the foundation for robust suppression strategies in the future. |
| first_indexed | 2024-12-14T06:29:58Z |
| format | Article |
| id | doaj.art-d361c4ed77dd4a8588a6c63ade2f1c01 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-14T06:29:58Z |
| publishDate | 2021-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-d361c4ed77dd4a8588a6c63ade2f1c012022-12-21T23:13:33ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352021-06-01156e000935710.1371/journal.pntd.0009357Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program.Brendan J TrewinDaniel E PagendamBrian J JohnsonChris PatonNigel SnoadScott A RitchieKyran M StauntonBradley J WhiteSara MitchellNigel W BeebeRapid advances in biological and digital support systems are revolutionizing the population control of invasive disease vectors such as Aedes aegypti. Methods such as the sterile and incompatible insect techniques (SIT/IIT) rely on modified males to seek out and successfully mate with females, and in doing so outcompete the wild male population for mates. Currently, these interventions most frequently infer mating success through area-wide population surveillance and estimates of mating competitiveness are rare. Furthermore, little is known about male Ae. aegypti behaviour and biology in field settings. In preparation for a large, community scale IIT program, we undertook a series of mark- release-recapture experiments using rhodamine B to mark male Ae. aegypti sperm and measure mating interactions with females. We also developed a Spatial and Temporally Evolving Isotropic Kernel (STEIK) framework to assist researchers to estimate the movement of individuals through space and time. Results showed that ~40% of wild females captured daily were unmated, suggesting interventions will need to release males multiple times per week to be effective at suppressing Ae. aegypti populations. Males moved rapidly through the landscape, particularly when released during the night. Although males moved further than what is typically observed in females of the species, survival was considerably lower. These unique insights improve our understanding of mating interactions in wild Ae. aegypti populations and lay the foundation for robust suppression strategies in the future.https://doi.org/10.1371/journal.pntd.0009357 |
| spellingShingle | Brendan J Trewin Daniel E Pagendam Brian J Johnson Chris Paton Nigel Snoad Scott A Ritchie Kyran M Staunton Bradley J White Sara Mitchell Nigel W Beebe Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program. PLoS Neglected Tropical Diseases |
| title | Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program. |
| title_full | Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program. |
| title_fullStr | Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program. |
| title_full_unstemmed | Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program. |
| title_short | Mark-release-recapture of male Aedes aegypti (Diptera: Culicidae): Use of rhodamine B to estimate movement, mating and population parameters in preparation for an incompatible male program. |
| title_sort | mark release recapture of male aedes aegypti diptera culicidae use of rhodamine b to estimate movement mating and population parameters in preparation for an incompatible male program |
| url | https://doi.org/10.1371/journal.pntd.0009357 |
| work_keys_str_mv | AT brendanjtrewin markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT danielepagendam markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT brianjjohnson markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT chrispaton markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT nigelsnoad markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT scottaritchie markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT kyranmstaunton markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT bradleyjwhite markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT saramitchell markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram AT nigelwbeebe markreleaserecaptureofmaleaedesaegyptidipteraculicidaeuseofrhodaminebtoestimatemovementmatingandpopulationparametersinpreparationforanincompatiblemaleprogram |