Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis
Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective o...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01174/full |
| _version_ | 1811287591847198720 |
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| author | Lingna Lyu Xiuli Zhang Xiuli Zhang Cuidan Li Cuidan Li Tingting Yang Tingting Yang Jinghui Wang Liping Pan Hongyan Jia Zihui Li Qi Sun Liya Yue Fei Chen Fei Chen Zongde Zhang |
| author_facet | Lingna Lyu Xiuli Zhang Xiuli Zhang Cuidan Li Cuidan Li Tingting Yang Tingting Yang Jinghui Wang Liping Pan Hongyan Jia Zihui Li Qi Sun Liya Yue Fei Chen Fei Chen Zongde Zhang |
| author_sort | Lingna Lyu |
| collection | DOAJ |
| description | Tuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective on investigation of the process of TB infection. In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC). Our results revealed distinct miRNA profile of the exosomes in the three groups. We screened 250 differentially expressed miRNAs including 130 specifically expressed miRNAs. Some miRNAs were further validated to be specifically expressed in LTBI (hsa-let-7e-5p, hsa-let-7d-5p, hsa-miR-450a-5p, and hsa-miR-140-5p) and TB samples (hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3P, hsa-miR-28-3p, and hsa-miR-193b-5p). Additionally, we demonstrated four expression panels in LTBI and TB groups, and six expression patterns among the three groups. These specifically expressed miRNAs and differentially expressed miRNAs in different panels and patterns provide potential biomarkers for detection/diagnosis of latent and active TB using exosomal miRNAs. Additionally, we also discovered plenty of small RNAs derived from genomic repetitive sequences, which might play roles in host immune responses along with Mtb infection progresses. Overall, our findings provide important reference and an improved understanding about miRNAs and repetitive region-derived small RNAs in exosomes during the Mtb infectious process, and facilitate the development of potential molecular targets for detection/diagnosis of latent and active tuberculosis. |
| first_indexed | 2024-04-13T03:20:47Z |
| format | Article |
| id | doaj.art-d364252aa11a4bf998a1e24b3f3ef186 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T03:20:47Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d364252aa11a4bf998a1e24b3f3ef1862022-12-22T03:04:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-05-011010.3389/fmicb.2019.01174444274Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active TuberculosisLingna Lyu0Xiuli Zhang1Xiuli Zhang2Cuidan Li3Cuidan Li4Tingting Yang5Tingting Yang6Jinghui Wang7Liping Pan8Hongyan Jia9Zihui Li10Qi Sun11Liya Yue12Fei Chen13Fei Chen14Zongde Zhang15Beijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaCAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaCAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, ChinaTuberculosis (TB) has been the leading lethal infectious disease worldwide since 2014, and about one third of the world’s population has a latent TB infection (LTBI). This is largely attributed to the difficulties in diagnosis and treatment of TB and LTBI patients. Exosomes offer a new perspective on investigation of the process of TB infection. In this study, we performed small RNA sequencing to explore small RNA profiles of serum exosomes derived from LTBI and TB patients and healthy controls (HC). Our results revealed distinct miRNA profile of the exosomes in the three groups. We screened 250 differentially expressed miRNAs including 130 specifically expressed miRNAs. Some miRNAs were further validated to be specifically expressed in LTBI (hsa-let-7e-5p, hsa-let-7d-5p, hsa-miR-450a-5p, and hsa-miR-140-5p) and TB samples (hsa-miR-1246, hsa-miR-2110, hsa-miR-370-3P, hsa-miR-28-3p, and hsa-miR-193b-5p). Additionally, we demonstrated four expression panels in LTBI and TB groups, and six expression patterns among the three groups. These specifically expressed miRNAs and differentially expressed miRNAs in different panels and patterns provide potential biomarkers for detection/diagnosis of latent and active TB using exosomal miRNAs. Additionally, we also discovered plenty of small RNAs derived from genomic repetitive sequences, which might play roles in host immune responses along with Mtb infection progresses. Overall, our findings provide important reference and an improved understanding about miRNAs and repetitive region-derived small RNAs in exosomes during the Mtb infectious process, and facilitate the development of potential molecular targets for detection/diagnosis of latent and active tuberculosis.https://www.frontiersin.org/article/10.3389/fmicb.2019.01174/fullexosomemiRNAtuberculosisRNA sequencingsmall RNAlatent TB infection |
| spellingShingle | Lingna Lyu Xiuli Zhang Xiuli Zhang Cuidan Li Cuidan Li Tingting Yang Tingting Yang Jinghui Wang Liping Pan Hongyan Jia Zihui Li Qi Sun Liya Yue Fei Chen Fei Chen Zongde Zhang Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis Frontiers in Microbiology exosome miRNA tuberculosis RNA sequencing small RNA latent TB infection |
| title | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
| title_full | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
| title_fullStr | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
| title_full_unstemmed | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
| title_short | Small RNA Profiles of Serum Exosomes Derived From Individuals With Latent and Active Tuberculosis |
| title_sort | small rna profiles of serum exosomes derived from individuals with latent and active tuberculosis |
| topic | exosome miRNA tuberculosis RNA sequencing small RNA latent TB infection |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01174/full |
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