Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity
Bangia fuscopurpurea is a widespread intertidal seaweed that is commercially cultured in China. This seaweed is frequently exposed to hyposalinity stress, but little is known about the adaptation mechanisms. Ascorbate−glutathione (AsA−GSH) cycle plays important roles in many organisms under a variet...
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| Format: | Article |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1174472/full |
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| author | Citong Niu Citong Niu Wenjun Wang Wenjun Wang Haiqin Yao Zhourui Liang Zhourui Liang Pengyan Zhang Xiaoping Lu |
| author_facet | Citong Niu Citong Niu Wenjun Wang Wenjun Wang Haiqin Yao Zhourui Liang Zhourui Liang Pengyan Zhang Xiaoping Lu |
| author_sort | Citong Niu |
| collection | DOAJ |
| description | Bangia fuscopurpurea is a widespread intertidal seaweed that is commercially cultured in China. This seaweed is frequently exposed to hyposalinity stress, but little is known about the adaptation mechanisms. Ascorbate−glutathione (AsA−GSH) cycle plays important roles in many organisms under a variety of abiotic stress, including hyposaline stress. In this study, we investigated the response of key metabolites and enzymes involved in the AsA−GSH cycle of B. fuscopurpurea under hyposalinity, with the addition of exogenous GSH and Lbuthionine-sulfoximine (BSO). The quantification of BfAPX gene expression was assessed across varied treatment regimens. And the putative interaction proteins of BfAPX were screened by yeast two hybrid system. It was found that under hyposalinity (15 and/or 0 psu), the content of reduced glutathione (GSH), total glutathione (GSH+ oxidized glutathione, GSSG) and cysteine, the ratio of GSH/GSSG and ascorbic acid (AsA)/ dehydroascorbic acid (DHA), and the activity of ascorbic acid peroxidase (APX) and monodehydroascorbate reductase (MDHAR) was significantly up-regulated. The hyposality-promoted GSH/GSSG was weakened while the glutathione reductase (GR) activity was promoted by adding exogenous GSH and BSO. The hyposality-promoted AsA/DHA ratio was strengthened by exogenous GSH but weakened by BSO. The dehydroascorbate reductase (DHAR) activity had no significant changes either with or without exogenous GSH under all salinities, while DHAR activity together with DHA content was enhanced by BSO. The expression of APX gene markedly increased under hyposalinity+BSO treatment. Putative interacting proteins of APX, including glutamate dehydrogenase 1a and fructose diphosphate aldolase, were identified through screening. The results indicated that the AsA−GSH cycle was involved in response of B. fuscopurpurea to hyposalinity by means of increasing GSH/GSSG ratio (through promoting GSH biosynthesis pathway and GSH regeneration from GSSG by GR catalyzation) and AsA/DHA ratio (promoting AsA regeneration through MDHAR). These findings would contribute to improve the aquaculture of this promising economic species and unveil how intertidal seaweeds address the global climate challenges. |
| first_indexed | 2024-04-09T18:24:50Z |
| format | Article |
| id | doaj.art-d36436e65dc64f108f018857e8e8012e |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-09T18:24:50Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj.art-d36436e65dc64f108f018857e8e8012e2023-04-12T04:42:36ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-04-011010.3389/fmars.2023.11744721174472Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinityCitong Niu0Citong Niu1Wenjun Wang2Wenjun Wang3Haiqin Yao4Zhourui Liang5Zhourui Liang6Pengyan Zhang7Xiaoping Lu8College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaKey Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaBangia fuscopurpurea is a widespread intertidal seaweed that is commercially cultured in China. This seaweed is frequently exposed to hyposalinity stress, but little is known about the adaptation mechanisms. Ascorbate−glutathione (AsA−GSH) cycle plays important roles in many organisms under a variety of abiotic stress, including hyposaline stress. In this study, we investigated the response of key metabolites and enzymes involved in the AsA−GSH cycle of B. fuscopurpurea under hyposalinity, with the addition of exogenous GSH and Lbuthionine-sulfoximine (BSO). The quantification of BfAPX gene expression was assessed across varied treatment regimens. And the putative interaction proteins of BfAPX were screened by yeast two hybrid system. It was found that under hyposalinity (15 and/or 0 psu), the content of reduced glutathione (GSH), total glutathione (GSH+ oxidized glutathione, GSSG) and cysteine, the ratio of GSH/GSSG and ascorbic acid (AsA)/ dehydroascorbic acid (DHA), and the activity of ascorbic acid peroxidase (APX) and monodehydroascorbate reductase (MDHAR) was significantly up-regulated. The hyposality-promoted GSH/GSSG was weakened while the glutathione reductase (GR) activity was promoted by adding exogenous GSH and BSO. The hyposality-promoted AsA/DHA ratio was strengthened by exogenous GSH but weakened by BSO. The dehydroascorbate reductase (DHAR) activity had no significant changes either with or without exogenous GSH under all salinities, while DHAR activity together with DHA content was enhanced by BSO. The expression of APX gene markedly increased under hyposalinity+BSO treatment. Putative interacting proteins of APX, including glutamate dehydrogenase 1a and fructose diphosphate aldolase, were identified through screening. The results indicated that the AsA−GSH cycle was involved in response of B. fuscopurpurea to hyposalinity by means of increasing GSH/GSSG ratio (through promoting GSH biosynthesis pathway and GSH regeneration from GSSG by GR catalyzation) and AsA/DHA ratio (promoting AsA regeneration through MDHAR). These findings would contribute to improve the aquaculture of this promising economic species and unveil how intertidal seaweeds address the global climate challenges.https://www.frontiersin.org/articles/10.3389/fmars.2023.1174472/fullantioxidantAsA–GSH cycleBangia fuscopurpureachlorophyll fluorescencehyposalinity stressinteraction protein |
| spellingShingle | Citong Niu Citong Niu Wenjun Wang Wenjun Wang Haiqin Yao Zhourui Liang Zhourui Liang Pengyan Zhang Xiaoping Lu Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity Frontiers in Marine Science antioxidant AsA–GSH cycle Bangia fuscopurpurea chlorophyll fluorescence hyposalinity stress interaction protein |
| title | Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity |
| title_full | Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity |
| title_fullStr | Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity |
| title_full_unstemmed | Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity |
| title_short | Ascorbate−glutathione cycle involving in response of Bangia fuscopurpurea (Bangiales, Rhodophyta) to hyposalinity |
| title_sort | ascorbate glutathione cycle involving in response of bangia fuscopurpurea bangiales rhodophyta to hyposalinity |
| topic | antioxidant AsA–GSH cycle Bangia fuscopurpurea chlorophyll fluorescence hyposalinity stress interaction protein |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1174472/full |
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