Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research
Many applications in plant biology requires editing genomes accurately including correcting point mutations, incorporation of single-nucleotide polymorphisms (SNPs), and introduction of multinucleotide insertion/deletions (indels) into a predetermined position in the genome. These types of modificat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2020-01-01
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| Series: | BioDesign Research |
| Online Access: | http://dx.doi.org/10.34133/2020/9350905 |
| _version_ | 1797285222625050624 |
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| author | Md. Mahmudul Hassan Guoliang Yuan Jin-Gui Chen Gerald A. Tuskan Xiaohan Yang |
| author_facet | Md. Mahmudul Hassan Guoliang Yuan Jin-Gui Chen Gerald A. Tuskan Xiaohan Yang |
| author_sort | Md. Mahmudul Hassan |
| collection | DOAJ |
| description | Many applications in plant biology requires editing genomes accurately including correcting point mutations, incorporation of single-nucleotide polymorphisms (SNPs), and introduction of multinucleotide insertion/deletions (indels) into a predetermined position in the genome. These types of modifications are possible using existing genome-editing technologies such as the CRISPR-Cas systems, which require induction of double-stranded breaks in the target DNA site and the supply of a donor DNA molecule that contains the desired edit sequence. However, low frequency of homologous recombination in plants and difficulty of delivering the donor DNA molecules make this process extremely inefficient. Another kind of technology known as base editing can perform precise editing; however, only certain types of modifications can be obtained, e.g., C/G-to-T/A and A/T-to-G/C. Recently, a new type of genome-editing technology, referred to as “prime editing,” has been developed, which can achieve various types of editing such as any base-to-base conversion, including both transitions (C→T, G→A, A→G, and T→C) and transversion mutations (C→A, C→G, G→C, G→T, A→C, A→T, T→A, and T→G), as well as small indels without the requirement for inducing double-stranded break in the DNA. Because prime editing has wide flexibility to achieve different types of edits in the genome, it holds a great potential for developing superior crops for various purposes, such as increasing yield, providing resistance to various abiotic and biotic stresses, and improving quality of plant product. In this review, we describe the prime editing technology and discuss its limitations and potential applications in plant biology research. |
| first_indexed | 2024-03-07T17:59:05Z |
| format | Article |
| id | doaj.art-d3663851462a48e2ae33ccdff22aa1b9 |
| institution | Directory Open Access Journal |
| issn | 2693-1257 |
| language | English |
| last_indexed | 2024-03-07T17:59:05Z |
| publishDate | 2020-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | BioDesign Research |
| spelling | doaj.art-d3663851462a48e2ae33ccdff22aa1b92024-03-02T11:13:23ZengAmerican Association for the Advancement of Science (AAAS)BioDesign Research2693-12572020-01-01202010.34133/2020/9350905Prime Editing Technology and Its Prospects for Future Applications in Plant Biology ResearchMd. Mahmudul Hassan0Guoliang Yuan1Jin-Gui Chen2Gerald A. Tuskan3Xiaohan Yang4Biosciences Division, Oak Ridge National Laboratory, Oak Ridge TN 37831, USA; Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA; Department of Genetics and Plant Breeding, Patuakhali Science and Technology University, Dumki, Patuakhali 8602, BangladeshBiosciences Division, Oak Ridge National Laboratory, Oak Ridge TN 37831, USA; Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USABiosciences Division, Oak Ridge National Laboratory, Oak Ridge TN 37831, USA; Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USABiosciences Division, Oak Ridge National Laboratory, Oak Ridge TN 37831, USA; Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USABiosciences Division, Oak Ridge National Laboratory, Oak Ridge TN 37831, USA; Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USAMany applications in plant biology requires editing genomes accurately including correcting point mutations, incorporation of single-nucleotide polymorphisms (SNPs), and introduction of multinucleotide insertion/deletions (indels) into a predetermined position in the genome. These types of modifications are possible using existing genome-editing technologies such as the CRISPR-Cas systems, which require induction of double-stranded breaks in the target DNA site and the supply of a donor DNA molecule that contains the desired edit sequence. However, low frequency of homologous recombination in plants and difficulty of delivering the donor DNA molecules make this process extremely inefficient. Another kind of technology known as base editing can perform precise editing; however, only certain types of modifications can be obtained, e.g., C/G-to-T/A and A/T-to-G/C. Recently, a new type of genome-editing technology, referred to as “prime editing,” has been developed, which can achieve various types of editing such as any base-to-base conversion, including both transitions (C→T, G→A, A→G, and T→C) and transversion mutations (C→A, C→G, G→C, G→T, A→C, A→T, T→A, and T→G), as well as small indels without the requirement for inducing double-stranded break in the DNA. Because prime editing has wide flexibility to achieve different types of edits in the genome, it holds a great potential for developing superior crops for various purposes, such as increasing yield, providing resistance to various abiotic and biotic stresses, and improving quality of plant product. In this review, we describe the prime editing technology and discuss its limitations and potential applications in plant biology research.http://dx.doi.org/10.34133/2020/9350905 |
| spellingShingle | Md. Mahmudul Hassan Guoliang Yuan Jin-Gui Chen Gerald A. Tuskan Xiaohan Yang Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research BioDesign Research |
| title | Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research |
| title_full | Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research |
| title_fullStr | Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research |
| title_full_unstemmed | Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research |
| title_short | Prime Editing Technology and Its Prospects for Future Applications in Plant Biology Research |
| title_sort | prime editing technology and its prospects for future applications in plant biology research |
| url | http://dx.doi.org/10.34133/2020/9350905 |
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