SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Milk
The aim of this work is to develop a device based on thin-layer chromatography coupled with surface-enhanced Raman spectroscopy (TLC-SERS) to analyze sulfamethoxazole (SMX) and trimethoprim (TMP) in commercial milk samples using chemometric tools. Samples were eluted in TLC plates, and a central com...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/12/528 |
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| author | Frederico Luis Felipe Soares Benedito Roberto de Alvarenga Junior Renato Lajarim Carneiro |
| author_facet | Frederico Luis Felipe Soares Benedito Roberto de Alvarenga Junior Renato Lajarim Carneiro |
| author_sort | Frederico Luis Felipe Soares |
| collection | DOAJ |
| description | The aim of this work is to develop a device based on thin-layer chromatography coupled with surface-enhanced Raman spectroscopy (TLC-SERS) to analyze sulfamethoxazole (SMX) and trimethoprim (TMP) in commercial milk samples using chemometric tools. Samples were eluted in TLC plates, and a central composite design (CCD) of two factors was performed to optimize the gold nanoparticle dispersion on TLC plates for SERS, aiming at the detection of both drugs at concentrations close to their maximum residual limits (MRLs). Following the optimization, hyperspectral images from the SERS were captured of the TLC plates. Multivariate curve resolution (MCR-ALS) and independent component analysis (ICA) chemometric techniques were used to extract the signals of the analytes. All the samples presented recovery values of 81–128% for TMP. The quantification of SMX was not possible due to SERS suppression by an interferent. However, it was possible to detect SMX at a concentration of two times the MRL (8.0 × 10<sup>−7</sup> mol·L<sup>−1</sup>). The results demonstrate that the TLC-SERS device is a potential tool for the quantification of TMP and the detection of SMX in milk. |
| first_indexed | 2024-03-09T17:12:43Z |
| format | Article |
| id | doaj.art-d3693952c1d443b880ce82462457f479 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T17:12:43Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-d3693952c1d443b880ce82462457f4792023-11-24T13:59:30ZengMDPI AGChemosensors2227-90402022-12-01101252810.3390/chemosensors10120528SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in MilkFrederico Luis Felipe Soares0Benedito Roberto de Alvarenga Junior1Renato Lajarim Carneiro2Department of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, São Carlos 13560-905, BrazilDepartment of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, São Carlos 13560-905, BrazilDepartment of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, São Carlos 13560-905, BrazilThe aim of this work is to develop a device based on thin-layer chromatography coupled with surface-enhanced Raman spectroscopy (TLC-SERS) to analyze sulfamethoxazole (SMX) and trimethoprim (TMP) in commercial milk samples using chemometric tools. Samples were eluted in TLC plates, and a central composite design (CCD) of two factors was performed to optimize the gold nanoparticle dispersion on TLC plates for SERS, aiming at the detection of both drugs at concentrations close to their maximum residual limits (MRLs). Following the optimization, hyperspectral images from the SERS were captured of the TLC plates. Multivariate curve resolution (MCR-ALS) and independent component analysis (ICA) chemometric techniques were used to extract the signals of the analytes. All the samples presented recovery values of 81–128% for TMP. The quantification of SMX was not possible due to SERS suppression by an interferent. However, it was possible to detect SMX at a concentration of two times the MRL (8.0 × 10<sup>−7</sup> mol·L<sup>−1</sup>). The results demonstrate that the TLC-SERS device is a potential tool for the quantification of TMP and the detection of SMX in milk.https://www.mdpi.com/2227-9040/10/12/528surface-enhanced Raman spectroscopychemometricshyperspectral imagethin-layer chromatographymilkmultivariate curve resolution |
| spellingShingle | Frederico Luis Felipe Soares Benedito Roberto de Alvarenga Junior Renato Lajarim Carneiro SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Milk Chemosensors surface-enhanced Raman spectroscopy chemometrics hyperspectral image thin-layer chromatography milk multivariate curve resolution |
| title | SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Milk |
| title_full | SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Milk |
| title_fullStr | SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Milk |
| title_full_unstemmed | SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Milk |
| title_short | SERS-TLC Device for Simultaneous Determination of Sulfamethoxazole and Trimethoprim in Milk |
| title_sort | sers tlc device for simultaneous determination of sulfamethoxazole and trimethoprim in milk |
| topic | surface-enhanced Raman spectroscopy chemometrics hyperspectral image thin-layer chromatography milk multivariate curve resolution |
| url | https://www.mdpi.com/2227-9040/10/12/528 |
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