The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis
The K<sup>+</sup> transporter KT/HAK/KUP (K<sup>+</sup> transporter/high-affinity K<sup>+</sup>/K<sup>+</sup> uptake) family has a critical effect on K<sup>+</sup> uptake and translocation in plants under different environmental conditions....
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MDPI AG
2023-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/13/2422 |
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| author | Hong Zhu Jiayu Guo Tao Ma Shuyan Liu Yuanyuan Zhou Xue Yang Qiyan Li Kaiyue Yu Tongshuai Wang Sixiang He Chunmei Zhao Jingshan Wang Jiongming Sui |
| author_facet | Hong Zhu Jiayu Guo Tao Ma Shuyan Liu Yuanyuan Zhou Xue Yang Qiyan Li Kaiyue Yu Tongshuai Wang Sixiang He Chunmei Zhao Jingshan Wang Jiongming Sui |
| author_sort | Hong Zhu |
| collection | DOAJ |
| description | The K<sup>+</sup> transporter KT/HAK/KUP (K<sup>+</sup> transporter/high-affinity K<sup>+</sup>/K<sup>+</sup> uptake) family has a critical effect on K<sup>+</sup> uptake and translocation in plants under different environmental conditions. However, the functional analysis of KT/HAK/KUP members in sweet potatoes is still limited. The present work reported the physiological activity of a new gene, <i>IbHAK11</i>, in the KT/HAK/KUP family in sweet potatoes. <i>IbHAK11</i> expression increased significantly in the low K<sup>+</sup>-tolerant line compared with the low K<sup>+</sup>-sensitive line following treatment with low K<sup>+</sup> concentrations. <i>IbHAK11</i> upregulation promoted root growth in <i>Arabidopsis</i> under low K<sup>+</sup> conditions. Under high saline stress, transgenic lines had superior growth and photosynthetic characteristics compared with the wild-type (WT). As for <i>IbHAK11</i>-overexpressing plants, activation of both the non-enzymatic and enzymatic reactive oxygen species (ROS) scavenging systems was observed. Therefore, <i>IbHAK11</i>-overexpressing plants had lower malondialdehyde (MDA) and ROS levels (including H<sub>2</sub>O<sub>2</sub> and O<sup>2−</sup>) compared with WT under salt-induced stress. We also found that under both low K<sup>+</sup> and high salinity conditions, overexpression of <i>IbHAK11</i> enhanced K<sup>+</sup> translocation from the root to the shoot and decreased Na<sup>+</sup> absorption in <i>Arabidopsis</i>. Consequently, IbHAK11 positively regulated K<sup>+</sup> deficiency and high salinity stresses by regulating K<sup>+</sup> translocation and Na<sup>+</sup> uptake, thus maintaining K<sup>+</sup>/Na<sup>+</sup> homeostasis in plants. |
| first_indexed | 2024-03-11T01:32:40Z |
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| id | doaj.art-d36fb0357c05493d8745669bfd61cae4 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T01:32:40Z |
| publishDate | 2023-06-01 |
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| series | Plants |
| spelling | doaj.art-d36fb0357c05493d8745669bfd61cae42023-11-18T17:17:14ZengMDPI AGPlants2223-77472023-06-011213242210.3390/plants12132422The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion HomeostasisHong Zhu0Jiayu Guo1Tao Ma2Shuyan Liu3Yuanyuan Zhou4Xue Yang5Qiyan Li6Kaiyue Yu7Tongshuai Wang8Sixiang He9Chunmei Zhao10Jingshan Wang11Jiongming Sui12College of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCrop research Institute, Shandong Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in Huang-Huai-Hai Region, Ministry of Agriculture and Rural Affairs, Jinan 250100, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agricultural University, Qingdao 266109, ChinaThe K<sup>+</sup> transporter KT/HAK/KUP (K<sup>+</sup> transporter/high-affinity K<sup>+</sup>/K<sup>+</sup> uptake) family has a critical effect on K<sup>+</sup> uptake and translocation in plants under different environmental conditions. However, the functional analysis of KT/HAK/KUP members in sweet potatoes is still limited. The present work reported the physiological activity of a new gene, <i>IbHAK11</i>, in the KT/HAK/KUP family in sweet potatoes. <i>IbHAK11</i> expression increased significantly in the low K<sup>+</sup>-tolerant line compared with the low K<sup>+</sup>-sensitive line following treatment with low K<sup>+</sup> concentrations. <i>IbHAK11</i> upregulation promoted root growth in <i>Arabidopsis</i> under low K<sup>+</sup> conditions. Under high saline stress, transgenic lines had superior growth and photosynthetic characteristics compared with the wild-type (WT). As for <i>IbHAK11</i>-overexpressing plants, activation of both the non-enzymatic and enzymatic reactive oxygen species (ROS) scavenging systems was observed. Therefore, <i>IbHAK11</i>-overexpressing plants had lower malondialdehyde (MDA) and ROS levels (including H<sub>2</sub>O<sub>2</sub> and O<sup>2−</sup>) compared with WT under salt-induced stress. We also found that under both low K<sup>+</sup> and high salinity conditions, overexpression of <i>IbHAK11</i> enhanced K<sup>+</sup> translocation from the root to the shoot and decreased Na<sup>+</sup> absorption in <i>Arabidopsis</i>. Consequently, IbHAK11 positively regulated K<sup>+</sup> deficiency and high salinity stresses by regulating K<sup>+</sup> translocation and Na<sup>+</sup> uptake, thus maintaining K<sup>+</sup>/Na<sup>+</sup> homeostasis in plants.https://www.mdpi.com/2223-7747/12/13/2422sweet potatoK<sup>+</sup> transporterIbHAK11K<sup>+</sup> deficiency tolerancehigh salinity toleranceK<sup>+</sup>/Na<sup>+</sup> homeostasis |
| spellingShingle | Hong Zhu Jiayu Guo Tao Ma Shuyan Liu Yuanyuan Zhou Xue Yang Qiyan Li Kaiyue Yu Tongshuai Wang Sixiang He Chunmei Zhao Jingshan Wang Jiongming Sui The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis Plants sweet potato K<sup>+</sup> transporter IbHAK11 K<sup>+</sup> deficiency tolerance high salinity tolerance K<sup>+</sup>/Na<sup>+</sup> homeostasis |
| title | The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis |
| title_full | The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis |
| title_fullStr | The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis |
| title_full_unstemmed | The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis |
| title_short | The Sweet Potato K<sup>+</sup> Transporter IbHAK11 Regulates K<sup>+</sup> Deficiency and High Salinity Stress Tolerance by Maintaining Positive Ion Homeostasis |
| title_sort | sweet potato k sup sup transporter ibhak11 regulates k sup sup deficiency and high salinity stress tolerance by maintaining positive ion homeostasis |
| topic | sweet potato K<sup>+</sup> transporter IbHAK11 K<sup>+</sup> deficiency tolerance high salinity tolerance K<sup>+</sup>/Na<sup>+</sup> homeostasis |
| url | https://www.mdpi.com/2223-7747/12/13/2422 |
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