Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogs
Abstract Background Guidelines‐driven screening protocols for early cancer detection in dogs are lacking, and cancer often is detected at advanced stages. Hypothesis/Objectives To examine how cancer typically is detected in dogs and whether the addition of a next‐generation sequencing‐based “liquid...
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16616 |
| _version_ | 1797937999476948992 |
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| author | Andi Flory Lisa McLennan Betsy Peet Marissa Kroll Deirdre Stuart Devon Brown Kathy Stuebner Brenda Phillips Brenda L. Coomber J. Paul Woods Mairin Miller Chelsea D. Tripp Amber Wolf‐Ringwall Kristina M. Kruglyak Angela L. McCleary‐Wheeler Ashley Phelps‐Dunn Lilian K. Wong Chelsea D. Warren Gina Brandstetter Michelle C. Rosentel Lauren R. DiMarzio Allison L. O'Kell Todd A. Cohen Daniel S. Grosu Jason Chibuk Dana W. Y. Tsui Ilya Chorny Jill M. Rafalko |
| author_facet | Andi Flory Lisa McLennan Betsy Peet Marissa Kroll Deirdre Stuart Devon Brown Kathy Stuebner Brenda Phillips Brenda L. Coomber J. Paul Woods Mairin Miller Chelsea D. Tripp Amber Wolf‐Ringwall Kristina M. Kruglyak Angela L. McCleary‐Wheeler Ashley Phelps‐Dunn Lilian K. Wong Chelsea D. Warren Gina Brandstetter Michelle C. Rosentel Lauren R. DiMarzio Allison L. O'Kell Todd A. Cohen Daniel S. Grosu Jason Chibuk Dana W. Y. Tsui Ilya Chorny Jill M. Rafalko |
| author_sort | Andi Flory |
| collection | DOAJ |
| description | Abstract Background Guidelines‐driven screening protocols for early cancer detection in dogs are lacking, and cancer often is detected at advanced stages. Hypothesis/Objectives To examine how cancer typically is detected in dogs and whether the addition of a next‐generation sequencing‐based “liquid biopsy” test to a wellness visit has the potential to enhance cancer detection. Animals Client‐owned dogs with definitive cancer diagnoses enrolled in a clinical validation study for a novel blood‐based multicancer early detection test. Methods Retrospective medical record review was performed to establish the history and presenting complaint that ultimately led to a definitive cancer diagnosis. Blood samples were subjected to DNA extraction, library preparation, and next‐generation sequencing. Sequencing data were analyzed using an internally developed bioinformatics pipeline to detect genomic alterations associated with the presence of cancer. Results In an unselected cohort of 359 cancer‐diagnosed dogs, 4% of cases were detected during a wellness visit, 8% were detected incidentally, and 88% were detected after the owner reported clinical signs suggestive of cancer. Liquid biopsy detected disease in 54.7% (95% confidence interval [CI], 49.5%‐59.8%) of patients, including 32% of dogs with early‐stage cancer, 48% of preclinical dogs, and 84% of dogs with advanced‐stage disease. Conclusions/Clinical Importance Most cases of cancer were diagnosed after the onset of clinical signs; only 4% of dogs had cancer detected using the current standard of care (i.e., wellness visit). Liquid biopsy has the potential to increase detection of cancer when added to a dog's wellness visit. |
| first_indexed | 2024-04-10T18:54:04Z |
| format | Article |
| id | doaj.art-d371ca1a414843748846f7200db24ee1 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-10T18:54:04Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-d371ca1a414843748846f7200db24ee12023-02-01T04:49:52ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762023-01-0137125826710.1111/jvim.16616Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogsAndi Flory0Lisa McLennan1Betsy Peet2Marissa Kroll3Deirdre Stuart4Devon Brown5Kathy Stuebner6Brenda Phillips7Brenda L. Coomber8J. Paul Woods9Mairin Miller10Chelsea D. Tripp11Amber Wolf‐Ringwall12Kristina M. Kruglyak13Angela L. McCleary‐Wheeler14Ashley Phelps‐Dunn15Lilian K. Wong16Chelsea D. Warren17Gina Brandstetter18Michelle C. Rosentel19Lauren R. DiMarzio20Allison L. O'Kell21Todd A. Cohen22Daniel S. Grosu23Jason Chibuk24Dana W. Y. Tsui25Ilya Chorny26Jill M. Rafalko27PetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USADepartment of Clinical Studies, Ontario Veterinary College University of Guelph Guelph Ontario CanadaBridge Animal Referral Center Edmonds Washington USAClinical Investigation Center University of Minnesota Saint Paul Minnesota USAVeterinary Specialty Hospital of San Diego San Diego California USADepartment of Biomedical Sciences, Ontario Veterinary College University of Guelph Guelph Ontario CanadaInstitute for Comparative Cancer Investigation at the Mona Campbell Centre for Animal Cancer, Ontario Veterinary College, University of Guelph Guelph Ontario CanadaVeterinary Specialty Hospital of North County San Marcos California USABridge Animal Referral Center Edmonds Washington USADepartment of Veterinary Clinical Sciences University of Minnesota, College of Veterinary Medicine Saint Paul Minnesota USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAPetDx, Inc La Jolla California USAAbstract Background Guidelines‐driven screening protocols for early cancer detection in dogs are lacking, and cancer often is detected at advanced stages. Hypothesis/Objectives To examine how cancer typically is detected in dogs and whether the addition of a next‐generation sequencing‐based “liquid biopsy” test to a wellness visit has the potential to enhance cancer detection. Animals Client‐owned dogs with definitive cancer diagnoses enrolled in a clinical validation study for a novel blood‐based multicancer early detection test. Methods Retrospective medical record review was performed to establish the history and presenting complaint that ultimately led to a definitive cancer diagnosis. Blood samples were subjected to DNA extraction, library preparation, and next‐generation sequencing. Sequencing data were analyzed using an internally developed bioinformatics pipeline to detect genomic alterations associated with the presence of cancer. Results In an unselected cohort of 359 cancer‐diagnosed dogs, 4% of cases were detected during a wellness visit, 8% were detected incidentally, and 88% were detected after the owner reported clinical signs suggestive of cancer. Liquid biopsy detected disease in 54.7% (95% confidence interval [CI], 49.5%‐59.8%) of patients, including 32% of dogs with early‐stage cancer, 48% of preclinical dogs, and 84% of dogs with advanced‐stage disease. Conclusions/Clinical Importance Most cases of cancer were diagnosed after the onset of clinical signs; only 4% of dogs had cancer detected using the current standard of care (i.e., wellness visit). Liquid biopsy has the potential to increase detection of cancer when added to a dog's wellness visit.https://doi.org/10.1111/jvim.16616cancer screeningclinical signsearly stageincidentalliquid biopsymulticancer early detection |
| spellingShingle | Andi Flory Lisa McLennan Betsy Peet Marissa Kroll Deirdre Stuart Devon Brown Kathy Stuebner Brenda Phillips Brenda L. Coomber J. Paul Woods Mairin Miller Chelsea D. Tripp Amber Wolf‐Ringwall Kristina M. Kruglyak Angela L. McCleary‐Wheeler Ashley Phelps‐Dunn Lilian K. Wong Chelsea D. Warren Gina Brandstetter Michelle C. Rosentel Lauren R. DiMarzio Allison L. O'Kell Todd A. Cohen Daniel S. Grosu Jason Chibuk Dana W. Y. Tsui Ilya Chorny Jill M. Rafalko Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogs Journal of Veterinary Internal Medicine cancer screening clinical signs early stage incidental liquid biopsy multicancer early detection |
| title | Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogs |
| title_full | Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogs |
| title_fullStr | Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogs |
| title_full_unstemmed | Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogs |
| title_short | Cancer detection in clinical practice and using blood‐based liquid biopsy: A retrospective audit of over 350 dogs |
| title_sort | cancer detection in clinical practice and using blood based liquid biopsy a retrospective audit of over 350 dogs |
| topic | cancer screening clinical signs early stage incidental liquid biopsy multicancer early detection |
| url | https://doi.org/10.1111/jvim.16616 |
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