2D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and Therapy
Self-assembly of 13 nm gold nanoparticles (AuNPs) engineered into 2D structures in solution using DNA tiles for their possible use for gene delivery and photothermal therapy is reported. The two different DNA tiles were constructed and the AuNPs coated with oligonucleotides possessing complementary...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2012-03-01
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| Series: | Nano Biomedicine and Engineering |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v4i1.p17-22 |
| _version_ | 1826994082230042624 |
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| author | Ali Yasin Sonay Kemal Keseroğlu Mustafa Culha |
| author_facet | Ali Yasin Sonay Kemal Keseroğlu Mustafa Culha |
| author_sort | Ali Yasin Sonay |
| collection | DOAJ |
| description | Self-assembly of 13 nm gold nanoparticles (AuNPs) engineered into 2D structures in solution using DNA tiles for their possible use for gene delivery and photothermal therapy is reported. The two different DNA tiles were constructed and the AuNPs coated with oligonucleotides possessing complementary sequence from the free ends were hybridized with the sticky ends of the tiles. The DNA tiles were bind to each other by mixing the tile structures without a heating and cooling step. The constructed nanostructures were 5 to 7 DNA tiles long since heat was not used to elongate them. When the DNA tiles were bound to the AuNPs, it was observed that AuNPs tend to stay in close proximity by filling the gaps between tiles. The stability of the constructed structures was tested against DNase, a DNA cleaving enzyme, for possible applications for gene delivery and photothermal therapy. It was found that the AuNP bound DNA tile structures resist the DNase cleavage up to eighty percent. Due to the presence of the AuNPs in the structure, the enzyme cannot bind to DNA sequences and this increases the DNA tile structures’ stability. |
| first_indexed | 2024-03-11T21:45:57Z |
| format | Article |
| id | doaj.art-d3727cf5358e4b22bb0f55fd4896e3dc |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2025-02-18T09:13:55Z |
| publishDate | 2012-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-d3727cf5358e4b22bb0f55fd4896e3dc2024-11-02T22:57:56ZengTsinghua University PressNano Biomedicine and Engineering2150-55782012-03-0141172210.5101/nbe.v4i1.p17-222D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and TherapyAli Yasin Sonay0Kemal Keseroğlu1Mustafa Culha2Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Ataşehir, Istanbul 34755, TurkeyDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Ataşehir, Istanbul 34755, TurkeyDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Ataşehir, Istanbul 34755, TurkeySelf-assembly of 13 nm gold nanoparticles (AuNPs) engineered into 2D structures in solution using DNA tiles for their possible use for gene delivery and photothermal therapy is reported. The two different DNA tiles were constructed and the AuNPs coated with oligonucleotides possessing complementary sequence from the free ends were hybridized with the sticky ends of the tiles. The DNA tiles were bind to each other by mixing the tile structures without a heating and cooling step. The constructed nanostructures were 5 to 7 DNA tiles long since heat was not used to elongate them. When the DNA tiles were bound to the AuNPs, it was observed that AuNPs tend to stay in close proximity by filling the gaps between tiles. The stability of the constructed structures was tested against DNase, a DNA cleaving enzyme, for possible applications for gene delivery and photothermal therapy. It was found that the AuNP bound DNA tile structures resist the DNase cleavage up to eighty percent. Due to the presence of the AuNPs in the structure, the enzyme cannot bind to DNA sequences and this increases the DNA tile structures’ stability.https://www.sciopen.com/article/10.5101/nbe.v4i1.p17-22self assemblydna tilegold nanoparticlesdelivery and photothermal therapy |
| spellingShingle | Ali Yasin Sonay Kemal Keseroğlu Mustafa Culha 2D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and Therapy Nano Biomedicine and Engineering self assembly dna tile gold nanoparticles delivery and photothermal therapy |
| title | 2D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and Therapy |
| title_full | 2D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and Therapy |
| title_fullStr | 2D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and Therapy |
| title_full_unstemmed | 2D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and Therapy |
| title_short | 2D Gold Nanoparticle Structures Engineered Through DNA Tiles for Delivery and Therapy |
| title_sort | 2d gold nanoparticle structures engineered through dna tiles for delivery and therapy |
| topic | self assembly dna tile gold nanoparticles delivery and photothermal therapy |
| url | https://www.sciopen.com/article/10.5101/nbe.v4i1.p17-22 |
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