The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length

The objective of the research were to find out the effect of fructose addition in semen extender to the quality of separation Garut ram sperm and their length viability (up to 40% motility) in cool storage. Research design was split plot with four treatments of fructose addition in coconut water ext...

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Main Authors: SD Rasad, LC Simanjuntak
Format: Article
Language:English
Published: Universitas Jenderal Soedirman (UNSOED), Faculty of Animal Science 2009-09-01
Series:Animal Production: Indonesian Journal of Animal Production
Subjects:
Online Access:http://animalproduction.net/index.php/JAP/article/view/245
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author SD Rasad
LC Simanjuntak
author_facet SD Rasad
LC Simanjuntak
author_sort SD Rasad
collection DOAJ
description The objective of the research were to find out the effect of fructose addition in semen extender to the quality of separation Garut ram sperm and their length viability (up to 40% motility) in cool storage. Research design was split plot with four treatments of fructose addition in coconut water extender as a main plot i.e.: 1% (P1); 1.25% (P2); 1.50 (P3) and 1.75% (P4) and the length of sperm viability in cool storage (0; 3rd ; 6 th;9 th;12 th and 15 th hour), as a subplot. Each treatment was repeated five times. Sperm separation was done by albumin column method with 10 % concentration in top layer and 30% in lower layer. Parameters of research were sperm motility and abnormality after separation. Research result showed that addition of 1.75% fructose has the best sperm motility of 57.18± 1.43% and abnormality of 5.76±0.20% in 12 hours cool storage (top layer of albumin column). Sperm motility of 57.62 ± 0.65 % and abnormality of 4.35 ± 0.25% in 12 hours cool storage (lower layer of albumin column). As a conclusion, addition of fructose in coconut water semen extender has positive influenced on sperm quality. (Animal Production 11(3): 196-201 (2009)Key Words: fructose, sperm quality, extender, Garut ram
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spelling doaj.art-d3752ece74da4d05a90b366c9f33e7122024-04-03T04:40:20ZengUniversitas Jenderal Soedirman (UNSOED), Faculty of Animal ScienceAnimal Production: Indonesian Journal of Animal Production1411-20272009-09-01113196201The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage LengthSD RasadLC SimanjuntakThe objective of the research were to find out the effect of fructose addition in semen extender to the quality of separation Garut ram sperm and their length viability (up to 40% motility) in cool storage. Research design was split plot with four treatments of fructose addition in coconut water extender as a main plot i.e.: 1% (P1); 1.25% (P2); 1.50 (P3) and 1.75% (P4) and the length of sperm viability in cool storage (0; 3rd ; 6 th;9 th;12 th and 15 th hour), as a subplot. Each treatment was repeated five times. Sperm separation was done by albumin column method with 10 % concentration in top layer and 30% in lower layer. Parameters of research were sperm motility and abnormality after separation. Research result showed that addition of 1.75% fructose has the best sperm motility of 57.18± 1.43% and abnormality of 5.76±0.20% in 12 hours cool storage (top layer of albumin column). Sperm motility of 57.62 ± 0.65 % and abnormality of 4.35 ± 0.25% in 12 hours cool storage (lower layer of albumin column). As a conclusion, addition of fructose in coconut water semen extender has positive influenced on sperm quality. (Animal Production 11(3): 196-201 (2009)Key Words: fructose, sperm quality, extender, Garut ramhttp://animalproduction.net/index.php/JAP/article/view/245fructosesperm qualityextenderGarut ram
spellingShingle SD Rasad
LC Simanjuntak
The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length
Animal Production: Indonesian Journal of Animal Production
fructose
sperm quality
extender
Garut ram
title The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length
title_full The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length
title_fullStr The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length
title_full_unstemmed The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length
title_short The Effect of Fructose Addition in Semen Extender on Quality of Separation of Garut Ram Sperm in Several Storage Length
title_sort effect of fructose addition in semen extender on quality of separation of garut ram sperm in several storage length
topic fructose
sperm quality
extender
Garut ram
url http://animalproduction.net/index.php/JAP/article/view/245
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AT sdrasad effectoffructoseadditioninsemenextenderonqualityofseparationofgarutramsperminseveralstoragelength
AT lcsimanjuntak effectoffructoseadditioninsemenextenderonqualityofseparationofgarutramsperminseveralstoragelength