Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro
In light of the promising effect of sodium trimetaphosphate nanoparticles (TMPn) on dental enamel, in addition to the scarce evidence of the effects of these nanoparticles on biofilms, this study evaluated the activity of TMPn with/without fluoride (F) on the pH, inorganic composition and extracellu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/1/170 |
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| author | Viviane de Oliveira Zequini Amarante Alberto Carlos Botazzo Delbem Caio Sampaio Leonardo Antônio de Morais Emerson Rodrigues de Camargo Douglas Roberto Monteiro Juliano Pelim Pessan Thayse Yumi Hosida |
| author_facet | Viviane de Oliveira Zequini Amarante Alberto Carlos Botazzo Delbem Caio Sampaio Leonardo Antônio de Morais Emerson Rodrigues de Camargo Douglas Roberto Monteiro Juliano Pelim Pessan Thayse Yumi Hosida |
| author_sort | Viviane de Oliveira Zequini Amarante |
| collection | DOAJ |
| description | In light of the promising effect of sodium trimetaphosphate nanoparticles (TMPn) on dental enamel, in addition to the scarce evidence of the effects of these nanoparticles on biofilms, this study evaluated the activity of TMPn with/without fluoride (F) on the pH, inorganic composition and extracellular matrix (ECM) components of dual-species biofilms of <i>Streptococcus mutans</i> and <i>Candida albicans</i>. The biofilms were cultivated in artificial saliva in microtiter plates and treated with solutions containing 1% or 3% conventional/microparticulate TMP (TMPm) or TMPn, with or without F. After the last treatment, the protein and carbohydrate content of the ECM was analyzed, and the pH and F, calcium (Ca), phosphorus (P), and TMP concentrations of the biofilms were determined. In another set of experiments, after the last treatment, the biofilms were exposed to a 20% sucrose solution, and their matrix composition, pH, and inorganic component contents were evaluated. 3% TMPn/F significantly reduced ECM carbohydrate and increased biofilm pH (after sucrose exposure) than other treatments. Also, it significantly increased P and F levels before sucrose exposure in comparison to 3% TMPm/F. In conclusion, 3% TMPn/F affected the biofilm ECM and pH, besides influencing inorganic biofilm composition by increasing P and F levels in the biofilm fluid. |
| first_indexed | 2024-03-09T12:05:08Z |
| format | Article |
| id | doaj.art-d377cbdbba4f4f31a5af4fa00e5c5b09 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T12:05:08Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-d377cbdbba4f4f31a5af4fa00e5c5b092023-11-30T22:59:43ZengMDPI AGNanomaterials2079-49912022-12-0113117010.3390/nano13010170Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In VitroViviane de Oliveira Zequini Amarante0Alberto Carlos Botazzo Delbem1Caio Sampaio2Leonardo Antônio de Morais3Emerson Rodrigues de Camargo4Douglas Roberto Monteiro5Juliano Pelim Pessan6Thayse Yumi Hosida7Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, BrazilDepartment of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, BrazilDepartment of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, BrazilDepartment of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, BrazilDepartment of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, SP, BrazilDepartment of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, BrazilDepartment of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, BrazilDepartment of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, BrazilIn light of the promising effect of sodium trimetaphosphate nanoparticles (TMPn) on dental enamel, in addition to the scarce evidence of the effects of these nanoparticles on biofilms, this study evaluated the activity of TMPn with/without fluoride (F) on the pH, inorganic composition and extracellular matrix (ECM) components of dual-species biofilms of <i>Streptococcus mutans</i> and <i>Candida albicans</i>. The biofilms were cultivated in artificial saliva in microtiter plates and treated with solutions containing 1% or 3% conventional/microparticulate TMP (TMPm) or TMPn, with or without F. After the last treatment, the protein and carbohydrate content of the ECM was analyzed, and the pH and F, calcium (Ca), phosphorus (P), and TMP concentrations of the biofilms were determined. In another set of experiments, after the last treatment, the biofilms were exposed to a 20% sucrose solution, and their matrix composition, pH, and inorganic component contents were evaluated. 3% TMPn/F significantly reduced ECM carbohydrate and increased biofilm pH (after sucrose exposure) than other treatments. Also, it significantly increased P and F levels before sucrose exposure in comparison to 3% TMPm/F. In conclusion, 3% TMPn/F affected the biofilm ECM and pH, besides influencing inorganic biofilm composition by increasing P and F levels in the biofilm fluid.https://www.mdpi.com/2079-4991/13/1/170phosphatesfluoridesnanotechnology<i>Streptococcus mutans</i><i>Candida albicans</i>dental caries |
| spellingShingle | Viviane de Oliveira Zequini Amarante Alberto Carlos Botazzo Delbem Caio Sampaio Leonardo Antônio de Morais Emerson Rodrigues de Camargo Douglas Roberto Monteiro Juliano Pelim Pessan Thayse Yumi Hosida Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro Nanomaterials phosphates fluorides nanotechnology <i>Streptococcus mutans</i> <i>Candida albicans</i> dental caries |
| title | Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro |
| title_full | Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro |
| title_fullStr | Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro |
| title_full_unstemmed | Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro |
| title_short | Activity of Sodium Trimetaphosphate Nanoparticles on Cariogenic-Related Biofilms In Vitro |
| title_sort | activity of sodium trimetaphosphate nanoparticles on cariogenic related biofilms in vitro |
| topic | phosphates fluorides nanotechnology <i>Streptococcus mutans</i> <i>Candida albicans</i> dental caries |
| url | https://www.mdpi.com/2079-4991/13/1/170 |
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