Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>Ga
Nanoparticles of Human Serum Albumin (NC) labelled with <sup>99m</sup>Tc are widely used in Nuclear Medicine and represent the gold-standard for the intraoperative detection of the sentinel lymph node in many kinds of cancer, mainly breast cancer and melanoma. A significant amount of rad...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/11/7/1776 |
| _version_ | 1797526483937263616 |
|---|---|
| author | Manuela Marenco Letizia Canziani Gianluca De Matteis Giorgio Cavenaghi Carlo Aprile Lorenzo Lodola |
| author_facet | Manuela Marenco Letizia Canziani Gianluca De Matteis Giorgio Cavenaghi Carlo Aprile Lorenzo Lodola |
| author_sort | Manuela Marenco |
| collection | DOAJ |
| description | Nanoparticles of Human Serum Albumin (NC) labelled with <sup>99m</sup>Tc are widely used in Nuclear Medicine and represent the gold-standard for the intraoperative detection of the sentinel lymph node in many kinds of cancer, mainly breast cancer and melanoma. A significant amount of radionuclides can be incorporated into the HSA particle, due to the multiple binding sites, and HSA-based nanocolloid catabolism is a fast and easy process that results in innocuous degradation products. NCs labelled with different isotopes represent an interesting radiopharmaceutical for extending diagnostic accuracy and surgical outcome, but the knowledge of the chemical bond between NCs and isotopes has not been fully elucidated, including information on its strength and specificity. The aim of this study is to investigate and compare the physicochemical characteristics of the bond between NCs and <sup>99m</sup>Tc and <sup>68</sup>Ga isotopes. Commercial kits of HSA-based nanocolloid particles (NanoAlbumon<sup>®</sup>) were used. For this purpose, we have primarily studied the kinetic orders of NC radiolabelling. Langmuir isotherms and pH effect on radiolabelling were tested and the stability of the radiometal complex was verified through competition reactions carried out in presence of different ligands. The future goal of our research is the development of inexpensive and instant kits, easily labelled with a wide spectrum of diagnostic and therapeutic isotopes, thus facilitating the availability of versatile and multipurpose radiopharmaceuticals. |
| first_indexed | 2024-03-10T09:30:55Z |
| format | Article |
| id | doaj.art-d377fbcaa78844f68d54cb37d260c628 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T09:30:55Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-d377fbcaa78844f68d54cb37d260c6282023-11-22T04:33:44ZengMDPI AGNanomaterials2079-49912021-07-01117177610.3390/nano11071776Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>GaManuela Marenco0Letizia Canziani1Gianluca De Matteis2Giorgio Cavenaghi3Carlo Aprile4Lorenzo Lodola5Fondazione IRCCS Policlinico San Matteo, Nuclear Medicine Unit, 27100 Pavia, ItalyFondazione IRCCS Policlinico San Matteo, Nuclear Medicine Unit, 27100 Pavia, ItalyFondazione IRCCS Policlinico San Matteo, Nuclear Medicine Unit, 27100 Pavia, ItalyFondazione IRCCS Policlinico San Matteo, Nuclear Medicine Unit, 27100 Pavia, ItalyNational Center for Oncological Hadrontherapy (CNAO), 27100 Pavia, ItalyFondazione IRCCS Policlinico San Matteo, Nuclear Medicine Unit, 27100 Pavia, ItalyNanoparticles of Human Serum Albumin (NC) labelled with <sup>99m</sup>Tc are widely used in Nuclear Medicine and represent the gold-standard for the intraoperative detection of the sentinel lymph node in many kinds of cancer, mainly breast cancer and melanoma. A significant amount of radionuclides can be incorporated into the HSA particle, due to the multiple binding sites, and HSA-based nanocolloid catabolism is a fast and easy process that results in innocuous degradation products. NCs labelled with different isotopes represent an interesting radiopharmaceutical for extending diagnostic accuracy and surgical outcome, but the knowledge of the chemical bond between NCs and isotopes has not been fully elucidated, including information on its strength and specificity. The aim of this study is to investigate and compare the physicochemical characteristics of the bond between NCs and <sup>99m</sup>Tc and <sup>68</sup>Ga isotopes. Commercial kits of HSA-based nanocolloid particles (NanoAlbumon<sup>®</sup>) were used. For this purpose, we have primarily studied the kinetic orders of NC radiolabelling. Langmuir isotherms and pH effect on radiolabelling were tested and the stability of the radiometal complex was verified through competition reactions carried out in presence of different ligands. The future goal of our research is the development of inexpensive and instant kits, easily labelled with a wide spectrum of diagnostic and therapeutic isotopes, thus facilitating the availability of versatile and multipurpose radiopharmaceuticals.https://www.mdpi.com/2079-4991/11/7/1776radiopharmaceuticalPET (positron emission tomography)SPECT (single-photon emission computed tomography)nanocolloidshuman serum albumin<sup>99m</sup>Tc |
| spellingShingle | Manuela Marenco Letizia Canziani Gianluca De Matteis Giorgio Cavenaghi Carlo Aprile Lorenzo Lodola Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>Ga Nanomaterials radiopharmaceutical PET (positron emission tomography) SPECT (single-photon emission computed tomography) nanocolloids human serum albumin <sup>99m</sup>Tc |
| title | Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>Ga |
| title_full | Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>Ga |
| title_fullStr | Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>Ga |
| title_full_unstemmed | Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>Ga |
| title_short | Chemical and Physical Characterisation of Human Serum Albumin Nanocolloids: Kinetics, Strength and Specificity of Bonds with <sup>99m</sup>Tc and <sup>68</sup>Ga |
| title_sort | chemical and physical characterisation of human serum albumin nanocolloids kinetics strength and specificity of bonds with sup 99m sup tc and sup 68 sup ga |
| topic | radiopharmaceutical PET (positron emission tomography) SPECT (single-photon emission computed tomography) nanocolloids human serum albumin <sup>99m</sup>Tc |
| url | https://www.mdpi.com/2079-4991/11/7/1776 |
| work_keys_str_mv | AT manuelamarenco chemicalandphysicalcharacterisationofhumanserumalbuminnanocolloidskineticsstrengthandspecificityofbondswithsup99msuptcandsup68supga AT letiziacanziani chemicalandphysicalcharacterisationofhumanserumalbuminnanocolloidskineticsstrengthandspecificityofbondswithsup99msuptcandsup68supga AT gianlucadematteis chemicalandphysicalcharacterisationofhumanserumalbuminnanocolloidskineticsstrengthandspecificityofbondswithsup99msuptcandsup68supga AT giorgiocavenaghi chemicalandphysicalcharacterisationofhumanserumalbuminnanocolloidskineticsstrengthandspecificityofbondswithsup99msuptcandsup68supga AT carloaprile chemicalandphysicalcharacterisationofhumanserumalbuminnanocolloidskineticsstrengthandspecificityofbondswithsup99msuptcandsup68supga AT lorenzolodola chemicalandphysicalcharacterisationofhumanserumalbuminnanocolloidskineticsstrengthandspecificityofbondswithsup99msuptcandsup68supga |