Silica Monolithic Capillary Columns for Hilic Separations
The monolithic capillary columns (0.1 mm x 150 mm) prepared by the acidic hydrolysis of tetramethoxysilane (TMOS) in the presence of polyethylene glycol (PEG) and urea were modified by zwitterionic stationary phases and evaluated under HILIC separation conditions by employing a mixture containing nu...
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| Format: | Article |
| Language: | English |
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University of Pannonia
2018-07-01
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| Series: | Hungarian Journal of Industry and Chemistry |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/hjic.2018.46.issue-1/hjic-2018-0009/hjic-2018-0009.xml?format=INT |
| _version_ | 1811301714892947456 |
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| author | Moravcová Dana Planeta Josef |
| author_facet | Moravcová Dana Planeta Josef |
| author_sort | Moravcová Dana |
| collection | DOAJ |
| description | The monolithic capillary columns (0.1 mm x 150 mm) prepared by the acidic hydrolysis of tetramethoxysilane (TMOS) in the presence of polyethylene glycol (PEG) and urea were modified by zwitterionic stationary phases and evaluated under HILIC separation conditions by employing a mixture containing nucleosides and nucleotides. The polymeric layer of zwitterions did not affect the high separation efficiency of the original silica monolith. The prepared zwitterionic columns exhibited high separation efficiencies in a range 61,000-289,000 theoretical plates/m for a 2- methacryloyloxyethyl phosphorylcholine-based stationary phase and in a range 59,000-135,000 theoretical plates/m for a [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide-based stationary phase under optimal separation conditions. The grafted layer of zwitterions on the silica monolithic surface also significantly improved the separation selectivity to compounds of interest |
| first_indexed | 2024-04-13T07:13:49Z |
| format | Article |
| id | doaj.art-d3789f118bf14db195a74c71ecdbeb28 |
| institution | Directory Open Access Journal |
| issn | 2450-5102 |
| language | English |
| last_indexed | 2024-04-13T07:13:49Z |
| publishDate | 2018-07-01 |
| publisher | University of Pannonia |
| record_format | Article |
| series | Hungarian Journal of Industry and Chemistry |
| spelling | doaj.art-d3789f118bf14db195a74c71ecdbeb282022-12-22T02:56:48ZengUniversity of PannoniaHungarian Journal of Industry and Chemistry2450-51022018-07-01461394210.1515/hjic-2018-0009hjic-2018-0009Silica Monolithic Capillary Columns for Hilic SeparationsMoravcová Dana0Planeta Josef1Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveri,Brno, Czech RepublicInstitute of Analytical Chemistry of the Czech Academy of Sciences, Veveri,Brno, Czech RepublicThe monolithic capillary columns (0.1 mm x 150 mm) prepared by the acidic hydrolysis of tetramethoxysilane (TMOS) in the presence of polyethylene glycol (PEG) and urea were modified by zwitterionic stationary phases and evaluated under HILIC separation conditions by employing a mixture containing nucleosides and nucleotides. The polymeric layer of zwitterions did not affect the high separation efficiency of the original silica monolith. The prepared zwitterionic columns exhibited high separation efficiencies in a range 61,000-289,000 theoretical plates/m for a 2- methacryloyloxyethyl phosphorylcholine-based stationary phase and in a range 59,000-135,000 theoretical plates/m for a [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide-based stationary phase under optimal separation conditions. The grafted layer of zwitterions on the silica monolithic surface also significantly improved the separation selectivity to compounds of interesthttp://www.degruyter.com/view/j/hjic.2018.46.issue-1/hjic-2018-0009/hjic-2018-0009.xml?format=INTHILICsilica monolithnucleosidenucleotidecapillary chromatography |
| spellingShingle | Moravcová Dana Planeta Josef Silica Monolithic Capillary Columns for Hilic Separations Hungarian Journal of Industry and Chemistry HILIC silica monolith nucleoside nucleotide capillary chromatography |
| title | Silica Monolithic Capillary Columns for Hilic Separations |
| title_full | Silica Monolithic Capillary Columns for Hilic Separations |
| title_fullStr | Silica Monolithic Capillary Columns for Hilic Separations |
| title_full_unstemmed | Silica Monolithic Capillary Columns for Hilic Separations |
| title_short | Silica Monolithic Capillary Columns for Hilic Separations |
| title_sort | silica monolithic capillary columns for hilic separations |
| topic | HILIC silica monolith nucleoside nucleotide capillary chromatography |
| url | http://www.degruyter.com/view/j/hjic.2018.46.issue-1/hjic-2018-0009/hjic-2018-0009.xml?format=INT |
| work_keys_str_mv | AT moravcovadana silicamonolithiccapillarycolumnsforhilicseparations AT planetajosef silicamonolithiccapillarycolumnsforhilicseparations |