Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their Mixture
This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of <i>Matricaria chamomilla</i> L. essential oils (EOs). EOs of <i>M. chamomilla&l...
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MDPI AG
2022-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/18/5850 |
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| author | Ahmed Qasem Hamza Assaggaf Domenico Montesano Zineb Khalil Samiah Hamad Al-Mijalli Aicha EL Baaboua Nasreddine El Omari Naoual El Menyiy Saad Bakrim Ryan A. Sheikh Mohammed Merae Alshahrani Ahmed Abdullah Al Awadh Gokhan Zengin Abdelhakim Bouyahya Hanae Naceiri Mrabti |
| author_facet | Ahmed Qasem Hamza Assaggaf Domenico Montesano Zineb Khalil Samiah Hamad Al-Mijalli Aicha EL Baaboua Nasreddine El Omari Naoual El Menyiy Saad Bakrim Ryan A. Sheikh Mohammed Merae Alshahrani Ahmed Abdullah Al Awadh Gokhan Zengin Abdelhakim Bouyahya Hanae Naceiri Mrabti |
| author_sort | Ahmed Qasem |
| collection | DOAJ |
| description | This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of <i>Matricaria chamomilla</i> L. essential oils (EOs). EOs of <i>M. chamomilla</i> were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography–mass spectrophotometry (GC-MS). The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The antidiabetic activity was performed in vitro using the enzyme inhibition test. The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and <i>β</i>-carotene bleaching assay. The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test. The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), <i>β</i>-myrcene (8.01%), and chamazulene (6.54%). MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC<sub>50</sub> ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL). The mixture exhibited the best radical scavenging activity, with an IC<sub>50</sub> of 533.89 ± 15.05 µg/mL. As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL). The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h. The impact of EOs on the studied species showed an excellent antimicrobial (<i>Staphylococcus aureus</i> ATCC 29213 (22.97 ± 0.16 mm)), antifungal (<i>Aspergillus niger</i> (18.13 ± 0.18 mm)) and anti-yeast (<i>Candida albicans</i> (21.07 ± 0.24 mm) effect against all the tested strains. The results obtained indicate that the EOs of <i>M. chamomilla</i> could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required. |
| first_indexed | 2024-03-09T23:02:23Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-09T23:02:23Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d378b88a805a4a35ba9d51af5b1a63f52023-11-23T17:59:57ZengMDPI AGMolecules1420-30492022-09-012718585010.3390/molecules27185850Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their MixtureAhmed Qasem0Hamza Assaggaf1Domenico Montesano2Zineb Khalil3Samiah Hamad Al-Mijalli4Aicha EL Baaboua5Nasreddine El Omari6Naoual El Menyiy7Saad Bakrim8Ryan A. Sheikh9Mohammed Merae Alshahrani10Ahmed Abdullah Al Awadh11Gokhan Zengin12Abdelhakim Bouyahya13Hanae Naceiri Mrabti14Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 21955, Saudi ArabiaLaboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, ItalyLaboratory of Medicinal Chemistry, Drug Sciences Research Center, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Rabat BP 6203, MoroccoDepartment of Biology, College of Sciences, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaBiology and Health Laboratory, Department of Biology, Faculty of Science, Abdelmalek Essaadi University, Tetouan 93000, MoroccoLaboratory of Histology, Embryology and Cytogenetic, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Taounate 34025, MoroccoLaboratory of Pharmacology, National Agency of Medicinal and Aromatic Plants, Taounate 34025, MoroccoGeo-Bio-Environment Engineering and Innovation Laboratory, Molecular Engineering, Biotechnologies and Innovation Team, Polydisciplinary Faculty of Taroudant, Ibn Zohr University, Agadir 80000, MoroccoBiochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, Najran 61441, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, Najran 61441, Saudi ArabiaDepartment of Biology, Science Faculty, Selcuk University, Konya 42250, TurkeyLaboratory of Human Pathologies Biology, Department of Biology, Faculty of Sciences, Mohammed V University in Rabat, Rabat BP 6203, MoroccoLaboratory of Pharmacology and Toxicology, Bio Pharmaceutical and Toxicological Analysis Research Team, Faculty of Medicine and Pharmacy, University Mohammed V in Rabat, Rabat BP 6203, MoroccoThis exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of <i>Matricaria chamomilla</i> L. essential oils (EOs). EOs of <i>M. chamomilla</i> were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography–mass spectrophotometry (GC-MS). The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The antidiabetic activity was performed in vitro using the enzyme inhibition test. The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and <i>β</i>-carotene bleaching assay. The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test. The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), <i>β</i>-myrcene (8.01%), and chamazulene (6.54%). MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC<sub>50</sub> ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL). The mixture exhibited the best radical scavenging activity, with an IC<sub>50</sub> of 533.89 ± 15.05 µg/mL. As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL). The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h. The impact of EOs on the studied species showed an excellent antimicrobial (<i>Staphylococcus aureus</i> ATCC 29213 (22.97 ± 0.16 mm)), antifungal (<i>Aspergillus niger</i> (18.13 ± 0.18 mm)) and anti-yeast (<i>Candida albicans</i> (21.07 ± 0.24 mm) effect against all the tested strains. The results obtained indicate that the EOs of <i>M. chamomilla</i> could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.https://www.mdpi.com/1420-3049/27/18/5850<i>Matricaria chamomilla</i> L.essential oilsantimicrobial activityantidiabetic activityanti-inflammatory effectantioxidant effect |
| spellingShingle | Ahmed Qasem Hamza Assaggaf Domenico Montesano Zineb Khalil Samiah Hamad Al-Mijalli Aicha EL Baaboua Nasreddine El Omari Naoual El Menyiy Saad Bakrim Ryan A. Sheikh Mohammed Merae Alshahrani Ahmed Abdullah Al Awadh Gokhan Zengin Abdelhakim Bouyahya Hanae Naceiri Mrabti Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their Mixture Molecules <i>Matricaria chamomilla</i> L. essential oils antimicrobial activity antidiabetic activity anti-inflammatory effect antioxidant effect |
| title | Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their Mixture |
| title_full | Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their Mixture |
| title_fullStr | Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their Mixture |
| title_full_unstemmed | Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their Mixture |
| title_short | Determination of Chemical Compounds and Investigation of Biological Properties of <i>Matricaria chamomilla</i> Essential Oils, Honey, and Their Mixture |
| title_sort | determination of chemical compounds and investigation of biological properties of i matricaria chamomilla i essential oils honey and their mixture |
| topic | <i>Matricaria chamomilla</i> L. essential oils antimicrobial activity antidiabetic activity anti-inflammatory effect antioxidant effect |
| url | https://www.mdpi.com/1420-3049/27/18/5850 |
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