Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic Sea
This work studied the potential biotechnological applications of a naviculoid diatom (IMA053) and a green microalga (<i>Tetraselmis marina</i> IMA043) isolated from the North Adriatic Sea. Water, methanol, and dichloromethane (DCM) extracts were prepared from microalgae biomass and evalu...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/20/3/207 |
| _version_ | 1797445769307881472 |
|---|---|
| author | Riccardo Trentin Luísa Custódio Maria João Rodrigues Emanuela Moschin Katia Sciuto José Paulo da Silva Isabella Moro |
| author_facet | Riccardo Trentin Luísa Custódio Maria João Rodrigues Emanuela Moschin Katia Sciuto José Paulo da Silva Isabella Moro |
| author_sort | Riccardo Trentin |
| collection | DOAJ |
| description | This work studied the potential biotechnological applications of a naviculoid diatom (IMA053) and a green microalga (<i>Tetraselmis marina</i> IMA043) isolated from the North Adriatic Sea. Water, methanol, and dichloromethane (DCM) extracts were prepared from microalgae biomass and evaluated for total phenolic content (TPC) and in vitro antioxidant properties. Biomass was profiled for fatty acid methyl esters (FAME) composition. The DCM extracts had the highest levels of total phenolics, with values of 40.58 and 86.14 mg GAE/g dry weight (DW in IMA053 and IMA043, respectively). The DCM extracts had a higher radical scavenging activity (RSA) than the water and methanol ones, especially those from IMA043, with RSAs of 99.65% toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) at 10 mg/mL, and of 103.43% against 2,2-diphenyl-1-picrylhydrazyl (DPPH) at 5 mg/mL. The DCM extract of IMA053 displayed relevant copper chelating properties (67.48% at 10 mg/mL), while the highest iron chelating activity was observed in the water extract of the same species (92.05% at 10 mg/mL). Both strains presented a high proportion of saturated (SFA) and monounsaturated (MUFA) fatty acids. The results suggested that these microalgae could be further explored as sources of natural antioxidants for the pharmaceutical and food industry and as feedstock for biofuel production. |
| first_indexed | 2024-03-09T13:31:35Z |
| format | Article |
| id | doaj.art-d378fe3b03bb435dbdb7919f132c0c86 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T13:31:35Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-d378fe3b03bb435dbdb7919f132c0c862023-11-30T21:18:18ZengMDPI AGMarine Drugs1660-33972022-03-0120320710.3390/md20030207Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic SeaRiccardo Trentin0Luísa Custódio1Maria João Rodrigues2Emanuela Moschin3Katia Sciuto4José Paulo da Silva5Isabella Moro6Centre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Ed. 7, Campus of Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Ed. 7, Campus of Gambelas, 8005-139 Faro, PortugalCentre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Ed. 7, Campus of Gambelas, 8005-139 Faro, PortugalDepartment of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, ItalyDepartment of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, Via Luigi Borsari 46, 44121 Ferrara, ItalyCentre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Ed. 7, Campus of Gambelas, 8005-139 Faro, PortugalDepartment of Biology, University of Padova, Via U. Bassi 58/B, 35131 Padova, ItalyThis work studied the potential biotechnological applications of a naviculoid diatom (IMA053) and a green microalga (<i>Tetraselmis marina</i> IMA043) isolated from the North Adriatic Sea. Water, methanol, and dichloromethane (DCM) extracts were prepared from microalgae biomass and evaluated for total phenolic content (TPC) and in vitro antioxidant properties. Biomass was profiled for fatty acid methyl esters (FAME) composition. The DCM extracts had the highest levels of total phenolics, with values of 40.58 and 86.14 mg GAE/g dry weight (DW in IMA053 and IMA043, respectively). The DCM extracts had a higher radical scavenging activity (RSA) than the water and methanol ones, especially those from IMA043, with RSAs of 99.65% toward 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) at 10 mg/mL, and of 103.43% against 2,2-diphenyl-1-picrylhydrazyl (DPPH) at 5 mg/mL. The DCM extract of IMA053 displayed relevant copper chelating properties (67.48% at 10 mg/mL), while the highest iron chelating activity was observed in the water extract of the same species (92.05% at 10 mg/mL). Both strains presented a high proportion of saturated (SFA) and monounsaturated (MUFA) fatty acids. The results suggested that these microalgae could be further explored as sources of natural antioxidants for the pharmaceutical and food industry and as feedstock for biofuel production.https://www.mdpi.com/1660-3397/20/3/207biodieselmicroalgal biotechnologynatural antioxidants |
| spellingShingle | Riccardo Trentin Luísa Custódio Maria João Rodrigues Emanuela Moschin Katia Sciuto José Paulo da Silva Isabella Moro Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic Sea Marine Drugs biodiesel microalgal biotechnology natural antioxidants |
| title | Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic Sea |
| title_full | Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic Sea |
| title_fullStr | Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic Sea |
| title_full_unstemmed | Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic Sea |
| title_short | Total Phenolic Levels, In Vitro Antioxidant Properties, and Fatty Acid Profile of Two Microalgae, <i>Tetraselmis marina</i> Strain IMA043 and Naviculoid Diatom Strain IMA053, Isolated from the North Adriatic Sea |
| title_sort | total phenolic levels in vitro antioxidant properties and fatty acid profile of two microalgae i tetraselmis marina i strain ima043 and naviculoid diatom strain ima053 isolated from the north adriatic sea |
| topic | biodiesel microalgal biotechnology natural antioxidants |
| url | https://www.mdpi.com/1660-3397/20/3/207 |
| work_keys_str_mv | AT riccardotrentin totalphenoliclevelsinvitroantioxidantpropertiesandfattyacidprofileoftwomicroalgaeitetraselmismarinaistrainima043andnaviculoiddiatomstrainima053isolatedfromthenorthadriaticsea AT luisacustodio totalphenoliclevelsinvitroantioxidantpropertiesandfattyacidprofileoftwomicroalgaeitetraselmismarinaistrainima043andnaviculoiddiatomstrainima053isolatedfromthenorthadriaticsea AT mariajoaorodrigues totalphenoliclevelsinvitroantioxidantpropertiesandfattyacidprofileoftwomicroalgaeitetraselmismarinaistrainima043andnaviculoiddiatomstrainima053isolatedfromthenorthadriaticsea AT emanuelamoschin totalphenoliclevelsinvitroantioxidantpropertiesandfattyacidprofileoftwomicroalgaeitetraselmismarinaistrainima043andnaviculoiddiatomstrainima053isolatedfromthenorthadriaticsea AT katiasciuto totalphenoliclevelsinvitroantioxidantpropertiesandfattyacidprofileoftwomicroalgaeitetraselmismarinaistrainima043andnaviculoiddiatomstrainima053isolatedfromthenorthadriaticsea AT josepaulodasilva totalphenoliclevelsinvitroantioxidantpropertiesandfattyacidprofileoftwomicroalgaeitetraselmismarinaistrainima043andnaviculoiddiatomstrainima053isolatedfromthenorthadriaticsea AT isabellamoro totalphenoliclevelsinvitroantioxidantpropertiesandfattyacidprofileoftwomicroalgaeitetraselmismarinaistrainima043andnaviculoiddiatomstrainima053isolatedfromthenorthadriaticsea |