Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico
Pathogenic strains of <i>Escherichia coli</i> threaten public health due to their virulence factors and antibiotic resistance. Additionally, the virulence of this bacterium varies by region depending on environmental conditions, agricultural practices, and the use of antibiotics and disi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/12/1789 |
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| author | Yessica Enciso-Martínez Edwin Barrios-Villa César O. Sepúlveda-Moreno Manuel G. Ballesteros-Monrreal Dora E. Valencia-Rivera Gustavo A. González-Aguilar Miguel A. Martínez-Téllez Jesús Fernando Ayala-Zavala |
| author_facet | Yessica Enciso-Martínez Edwin Barrios-Villa César O. Sepúlveda-Moreno Manuel G. Ballesteros-Monrreal Dora E. Valencia-Rivera Gustavo A. González-Aguilar Miguel A. Martínez-Téllez Jesús Fernando Ayala-Zavala |
| author_sort | Yessica Enciso-Martínez |
| collection | DOAJ |
| description | Pathogenic strains of <i>Escherichia coli</i> threaten public health due to their virulence factors and antibiotic resistance. Additionally, the virulence of this bacterium varies by region depending on environmental conditions, agricultural practices, and the use of antibiotics and disinfectants. However, there is limited research on the prevalence of antibiotic-resistant <i>E. coli</i> in agriculture. Therefore, this research aimed to determine the antibiotic resistance of <i>E. coli</i> isolated from the Honeydew melon production system in Hermosillo, Sonora, Mexico. Thirty-two <i>E. coli</i> strains were isolated from 445 samples obtained from irrigation water, harvested melons, the hands of packaging workers, boxes, and discarded melons. The resistance profile of the <i>E. coli</i> strains was carried out to 12 antibiotics used in antimicrobial therapeutics against this bacterium; a high level of resistance to ertapenem (100%) was detected, followed by meropenem (97%), and ampicillin (94%); 47% of the strains were classified as multidrug-resistant. It was possible to identify the prevalence of the extended-spectrum β-lactamase (ESBLs) gene <i>bla<sub>TEM</sub></i> (15.6%), as well as the non-ESBL genes <i>qepA</i> (3.1%) and <i>aac(6′)lb-cr</i> (3.1%). The <i>E. coli</i> strains isolated from irrigation water were significantly associated with resistance to aztreonam, cefuroxime, amikacin, and sulfamethoxazole/trimethoprim. Irrigation water, packing workers’ hands, and discarded melons showed a higher prevalence of antibiotic-resistant, ESBL, and non-ESBL genes of <i>E. coli</i> strains in a farm and packing facility of Honeydew melon in Hermosillo, Sonora. |
| first_indexed | 2024-03-09T17:24:27Z |
| format | Article |
| id | doaj.art-d37abebd27724b0b81d3e26ed63f249d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T17:24:27Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d37abebd27724b0b81d3e26ed63f249d2023-11-24T12:54:26ZengMDPI AGAntibiotics2079-63822022-12-011112178910.3390/antibiotics11121789Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, MexicoYessica Enciso-Martínez0Edwin Barrios-Villa1César O. Sepúlveda-Moreno2Manuel G. Ballesteros-Monrreal3Dora E. Valencia-Rivera4Gustavo A. González-Aguilar5Miguel A. Martínez-Téllez6Jesús Fernando Ayala-Zavala7Coordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo 83304, MexicoDepartamento de Ciencias Químico-Biológicas y Agropecuarias, Universidad de Sonora, Unidad Regional Norte, Campus Caborca, Caborca 83621, MexicoDepartamento de Ciencias Económico-Administrativas, Universidad de Sonora, Unidad Regional Norte, Campus Caborca. H., Caborca 83621, MexicoDepartamento de Ciencias Químico-Biológicas y Agropecuarias, Universidad de Sonora, Unidad Regional Norte, Campus Caborca, Caborca 83621, MexicoDepartamento de Ciencias Químico-Biológicas y Agropecuarias, Universidad de Sonora, Unidad Regional Norte, Campus Caborca, Caborca 83621, MexicoCoordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo 83304, MexicoCoordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo 83304, MexicoCoordinación de Tecnología de Alimentos de Origen Vegetal, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo 83304, MexicoPathogenic strains of <i>Escherichia coli</i> threaten public health due to their virulence factors and antibiotic resistance. Additionally, the virulence of this bacterium varies by region depending on environmental conditions, agricultural practices, and the use of antibiotics and disinfectants. However, there is limited research on the prevalence of antibiotic-resistant <i>E. coli</i> in agriculture. Therefore, this research aimed to determine the antibiotic resistance of <i>E. coli</i> isolated from the Honeydew melon production system in Hermosillo, Sonora, Mexico. Thirty-two <i>E. coli</i> strains were isolated from 445 samples obtained from irrigation water, harvested melons, the hands of packaging workers, boxes, and discarded melons. The resistance profile of the <i>E. coli</i> strains was carried out to 12 antibiotics used in antimicrobial therapeutics against this bacterium; a high level of resistance to ertapenem (100%) was detected, followed by meropenem (97%), and ampicillin (94%); 47% of the strains were classified as multidrug-resistant. It was possible to identify the prevalence of the extended-spectrum β-lactamase (ESBLs) gene <i>bla<sub>TEM</sub></i> (15.6%), as well as the non-ESBL genes <i>qepA</i> (3.1%) and <i>aac(6′)lb-cr</i> (3.1%). The <i>E. coli</i> strains isolated from irrigation water were significantly associated with resistance to aztreonam, cefuroxime, amikacin, and sulfamethoxazole/trimethoprim. Irrigation water, packing workers’ hands, and discarded melons showed a higher prevalence of antibiotic-resistant, ESBL, and non-ESBL genes of <i>E. coli</i> strains in a farm and packing facility of Honeydew melon in Hermosillo, Sonora.https://www.mdpi.com/2079-6382/11/12/1789antibioticsfood safetypathogenic strainsmultidrug-resistance bacteria |
| spellingShingle | Yessica Enciso-Martínez Edwin Barrios-Villa César O. Sepúlveda-Moreno Manuel G. Ballesteros-Monrreal Dora E. Valencia-Rivera Gustavo A. González-Aguilar Miguel A. Martínez-Téllez Jesús Fernando Ayala-Zavala Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico Antibiotics antibiotics food safety pathogenic strains multidrug-resistance bacteria |
| title | Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico |
| title_full | Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico |
| title_fullStr | Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico |
| title_full_unstemmed | Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico |
| title_short | Prevalence of Antibiotic-Resistant <i>E. coli</i> Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico |
| title_sort | prevalence of antibiotic resistant i e coli i strains in a local farm and packing facilities of honeydew melon in hermosillo sonora mexico |
| topic | antibiotics food safety pathogenic strains multidrug-resistance bacteria |
| url | https://www.mdpi.com/2079-6382/11/12/1789 |
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