Bactericidal and disinfective properties of disinfectant «GK-10»
The article presents the results of research on bactericidal properties of the new domestic disinfectant «GK-10», which is a transparent solution of light yellow color, without mechanical inclusions with a specific odor. It is well mixed with water. It is based on alkyl dimethylbenzyl ammonium chlor...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
2018-04-01
|
| Series: | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки |
| Subjects: | |
| Online Access: | https://nvlvet.com.ua/index.php/journal/article/view/1582 |
| _version_ | 1818325465887145984 |
|---|---|
| author | O.L. Tishyn R.V. Khomiak G.T. Kopijchuk M.M. Danko S.A. Ponomariova |
| author_facet | O.L. Tishyn R.V. Khomiak G.T. Kopijchuk M.M. Danko S.A. Ponomariova |
| author_sort | O.L. Tishyn |
| collection | DOAJ |
| description | The article presents the results of research on bactericidal properties of the new domestic disinfectant «GK-10», which is a transparent solution of light yellow color, without mechanical inclusions with a specific odor. It is well mixed with water. It is based on alkyl dimethylbenzyl ammonium chloride (QAC) and dodecylbispropylentriamine (1,3-prodadiene) (tertiary amines). Was found bactericidal dilution (BD), bactericidal concentration (BC), phenolic coefficient (FC) and protein index (PI). Thus, the most susceptible to the action of the disinfectant were were gram-negative bacteria E. coli, where cell death occurred at 10 and 30 minute exposures at concentrations of 0.19 and 0.14%, respectively. Due to the action of the disinfectant on the gram-positive bacteria S. aureus, cell death came at these exposures at concentrations 0.27 and 0.19%, respectively. The least susceptible to the action of the disinfectant were vegetative forms of B. subtilis, the death of these bacteria came at concentrations 0.37 and 0.27% at 10 and 30 minutes of exposure, respectively. Effects of disinfectant was over 5.38 and 2.74 times higher on microorganisms of E. coli and S. aureus, respectively, of the decontamination effect of phenol, and in the presence of protein, the activity of the disinfectant is reduced by 6.45 times. The efficiency of the disinfectant for the strains of microorganisms at the disinfestation of the test-object surfaces is determined. It has been established that for test cultures of E. coli and S. aureus 0.05–0.3% of disinfectant concentration is ineffective, and 0.5% its disinfectant concentration for disinfection of hard surfaces in preventive disinfection of premises for animals is insufficient for exposure in 120 minutes. For spore form B. subtilis 1.0% disinfectant concentration was ineffective. 1.0% concentration is effective for aseptic cleaning: slaughterhouses, processing shops, laboratory facilities, means of transporting products of animal origin, soaking clothes before washing, and also for treating surfaces of wood, metal and glazed tile at exposures of 60 minutes and more, and when spore forms of microorganisms, the working concentration of insoluble in disinfection of objects subject to veterinary control should be 3% and above. It has been proved that the disinfectant «GK-10» is effective at a concentration of 0.5% at exposure for 180 minutes and at a concentration of 1.0% at an exposure of 120 minutes for the sanation and preventive disinfection of premises for animals. |
| first_indexed | 2024-12-13T11:44:55Z |
| format | Article |
| id | doaj.art-d37e342132974f5496b2a4e77d94ef75 |
| institution | Directory Open Access Journal |
| issn | 2518-7554 2518-1327 |
| language | English |
| last_indexed | 2024-12-13T11:44:55Z |
| publishDate | 2018-04-01 |
| publisher | Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv |
| record_format | Article |
| series | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки |
| spelling | doaj.art-d37e342132974f5496b2a4e77d94ef752022-12-21T23:47:32ZengStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies LvivНауковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки2518-75542518-13272018-04-0120873710.15421/nvlvet87011582Bactericidal and disinfective properties of disinfectant «GK-10»O.L. Tishyn0R.V. Khomiak1G.T. Kopijchuk2M.M. Danko3S.A. Ponomariova4State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, LvivState Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, LvivState Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, LvivState Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, LvivState Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives, LvivThe article presents the results of research on bactericidal properties of the new domestic disinfectant «GK-10», which is a transparent solution of light yellow color, without mechanical inclusions with a specific odor. It is well mixed with water. It is based on alkyl dimethylbenzyl ammonium chloride (QAC) and dodecylbispropylentriamine (1,3-prodadiene) (tertiary amines). Was found bactericidal dilution (BD), bactericidal concentration (BC), phenolic coefficient (FC) and protein index (PI). Thus, the most susceptible to the action of the disinfectant were were gram-negative bacteria E. coli, where cell death occurred at 10 and 30 minute exposures at concentrations of 0.19 and 0.14%, respectively. Due to the action of the disinfectant on the gram-positive bacteria S. aureus, cell death came at these exposures at concentrations 0.27 and 0.19%, respectively. The least susceptible to the action of the disinfectant were vegetative forms of B. subtilis, the death of these bacteria came at concentrations 0.37 and 0.27% at 10 and 30 minutes of exposure, respectively. Effects of disinfectant was over 5.38 and 2.74 times higher on microorganisms of E. coli and S. aureus, respectively, of the decontamination effect of phenol, and in the presence of protein, the activity of the disinfectant is reduced by 6.45 times. The efficiency of the disinfectant for the strains of microorganisms at the disinfestation of the test-object surfaces is determined. It has been established that for test cultures of E. coli and S. aureus 0.05–0.3% of disinfectant concentration is ineffective, and 0.5% its disinfectant concentration for disinfection of hard surfaces in preventive disinfection of premises for animals is insufficient for exposure in 120 minutes. For spore form B. subtilis 1.0% disinfectant concentration was ineffective. 1.0% concentration is effective for aseptic cleaning: slaughterhouses, processing shops, laboratory facilities, means of transporting products of animal origin, soaking clothes before washing, and also for treating surfaces of wood, metal and glazed tile at exposures of 60 minutes and more, and when spore forms of microorganisms, the working concentration of insoluble in disinfection of objects subject to veterinary control should be 3% and above. It has been proved that the disinfectant «GK-10» is effective at a concentration of 0.5% at exposure for 180 minutes and at a concentration of 1.0% at an exposure of 120 minutes for the sanation and preventive disinfection of premises for animals.https://nvlvet.com.ua/index.php/journal/article/view/1582desinfectang «gk-10», test-cultures, bactericidal dilution, bactericidal concentration, phenol coefficient, protein index, test-objects, desinfection |
| spellingShingle | O.L. Tishyn R.V. Khomiak G.T. Kopijchuk M.M. Danko S.A. Ponomariova Bactericidal and disinfective properties of disinfectant «GK-10» Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки desinfectang «gk-10», test-cultures, bactericidal dilution, bactericidal concentration, phenol coefficient, protein index, test-objects, desinfection |
| title | Bactericidal and disinfective properties of disinfectant «GK-10» |
| title_full | Bactericidal and disinfective properties of disinfectant «GK-10» |
| title_fullStr | Bactericidal and disinfective properties of disinfectant «GK-10» |
| title_full_unstemmed | Bactericidal and disinfective properties of disinfectant «GK-10» |
| title_short | Bactericidal and disinfective properties of disinfectant «GK-10» |
| title_sort | bactericidal and disinfective properties of disinfectant gk 10 |
| topic | desinfectang «gk-10», test-cultures, bactericidal dilution, bactericidal concentration, phenol coefficient, protein index, test-objects, desinfection |
| url | https://nvlvet.com.ua/index.php/journal/article/view/1582 |
| work_keys_str_mv | AT oltishyn bactericidalanddisinfectivepropertiesofdisinfectantgk10 AT rvkhomiak bactericidalanddisinfectivepropertiesofdisinfectantgk10 AT gtkopijchuk bactericidalanddisinfectivepropertiesofdisinfectantgk10 AT mmdanko bactericidalanddisinfectivepropertiesofdisinfectantgk10 AT saponomariova bactericidalanddisinfectivepropertiesofdisinfectantgk10 |