Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birds
<p>Abstract</p> <p>Background</p> <p><it>Mycobacterium avium </it>includes the subspecies <it>avium</it>, <it>silvaticum</it>, <it>paratuberculosis </it>and <it>hominissuis</it>, and <it>M. avium </it&...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-08-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/159 |
| _version_ | 1830302424989958144 |
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| author | Nilsen Sigrun Olsen Ingrid Agdestein Angelika Johansen Tone Holstad Gudmund Djønne Berit |
| author_facet | Nilsen Sigrun Olsen Ingrid Agdestein Angelika Johansen Tone Holstad Gudmund Djønne Berit |
| author_sort | Nilsen Sigrun |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Mycobacterium avium </it>includes the subspecies <it>avium</it>, <it>silvaticum</it>, <it>paratuberculosis </it>and <it>hominissuis</it>, and <it>M. avium </it>subspecies has been isolated from various environments all over the world including from biofilms in water distribution systems. The aim of this study was to examine isolates of <it>M. avium </it>subsp. <it>avium </it>and <it>M. avium </it>subsp. <it>hominissuis </it>of different origin for biofilm formation and to look for correlations between biofilm formation and RFLP-types, and to standardise the method to test for biofilm formation. In order to determine the best screening method, a panel of 14 isolates of <it>M. avium </it>subsp. <it>avium </it>and <it>M. avium </it>subsp. <it>hominissuis</it>, were tested for their ability to form biofilm in microtiter plates under different conditions. Subsequently, 83 additional isolates from humans, swine and birds were tested for biofilm formation. The isolates were tested for the presence of selected genes involved in the synthesis of glycopeptidolipids (GPLs) in the cell wall of <it>M. avium</it>, which is believed to be important for biofilm formation. Colony morphology and <it>hsp65 </it>sequvar were also determined.</p> <p>Results</p> <p>Nine isolates from swine produced biofilm. There was a significant higher frequency of porcine isolates forming biofilm compared to human isolates. All isolates were previously characterised by IS<it>1311</it>- and IS<it>1245</it>-RFLP typing. The ability to form biofilm did not correlate with the RFLP-type, <it>hsp65 </it>sequevar, colony morphology or the presence of gene sequences related to GPL synthesis.</p> <p>Conclusion</p> <p>The observed differences in biofilm forming abilities between porcine and human isolates raises questions regarding the importance of biofilm formation for infectious potential. The optimised method worked well for screening of multiple isolates.</p> |
| first_indexed | 2024-12-19T09:07:24Z |
| format | Article |
| id | doaj.art-d380433fa9234c9683482a9e320b11ee |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-19T09:07:24Z |
| publishDate | 2009-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-d380433fa9234c9683482a9e320b11ee2022-12-21T20:28:19ZengBMCBMC Microbiology1471-21802009-08-019115910.1186/1471-2180-9-159Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birdsNilsen SigrunOlsen IngridAgdestein AngelikaJohansen ToneHolstad GudmundDjønne Berit<p>Abstract</p> <p>Background</p> <p><it>Mycobacterium avium </it>includes the subspecies <it>avium</it>, <it>silvaticum</it>, <it>paratuberculosis </it>and <it>hominissuis</it>, and <it>M. avium </it>subspecies has been isolated from various environments all over the world including from biofilms in water distribution systems. The aim of this study was to examine isolates of <it>M. avium </it>subsp. <it>avium </it>and <it>M. avium </it>subsp. <it>hominissuis </it>of different origin for biofilm formation and to look for correlations between biofilm formation and RFLP-types, and to standardise the method to test for biofilm formation. In order to determine the best screening method, a panel of 14 isolates of <it>M. avium </it>subsp. <it>avium </it>and <it>M. avium </it>subsp. <it>hominissuis</it>, were tested for their ability to form biofilm in microtiter plates under different conditions. Subsequently, 83 additional isolates from humans, swine and birds were tested for biofilm formation. The isolates were tested for the presence of selected genes involved in the synthesis of glycopeptidolipids (GPLs) in the cell wall of <it>M. avium</it>, which is believed to be important for biofilm formation. Colony morphology and <it>hsp65 </it>sequvar were also determined.</p> <p>Results</p> <p>Nine isolates from swine produced biofilm. There was a significant higher frequency of porcine isolates forming biofilm compared to human isolates. All isolates were previously characterised by IS<it>1311</it>- and IS<it>1245</it>-RFLP typing. The ability to form biofilm did not correlate with the RFLP-type, <it>hsp65 </it>sequevar, colony morphology or the presence of gene sequences related to GPL synthesis.</p> <p>Conclusion</p> <p>The observed differences in biofilm forming abilities between porcine and human isolates raises questions regarding the importance of biofilm formation for infectious potential. The optimised method worked well for screening of multiple isolates.</p>http://www.biomedcentral.com/1471-2180/9/159 |
| spellingShingle | Nilsen Sigrun Olsen Ingrid Agdestein Angelika Johansen Tone Holstad Gudmund Djønne Berit Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birds BMC Microbiology |
| title | Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birds |
| title_full | Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birds |
| title_fullStr | Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birds |
| title_full_unstemmed | Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birds |
| title_short | Biofilm formation by <it>Mycobacterium avium </it>isolates originating from humans, swine and birds |
| title_sort | biofilm formation by it mycobacterium avium it isolates originating from humans swine and birds |
| url | http://www.biomedcentral.com/1471-2180/9/159 |
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