Genetics techniques for Thermococcus kodakarensis
Thermococcus kodakarensis (T.k.) has emerged as a premier model system for studies of archaeal biochemistry, genetics, and hyperthermophily. This prominence is derived largely from the natural competence of T.k. and the comprehensive, rapid, and facile techniques available for manipulation of the T...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00195/full |
| _version_ | 1828473680250798080 |
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| author | Travis H. Hileman Thomas James Santangelo |
| author_facet | Travis H. Hileman Thomas James Santangelo |
| author_sort | Travis H. Hileman |
| collection | DOAJ |
| description | Thermococcus kodakarensis (T.k.) has emerged as a premier model system for studies of archaeal biochemistry, genetics, and hyperthermophily. This prominence is derived largely from the natural competence of T.k. and the comprehensive, rapid, and facile techniques available for manipulation of the T.k. genome. These genetic capacities are complemented by robust planktonic growth, simple selections and screens, defined in vitro transcription and translation systems, replicative expression plasmids, in vivo reporter constructs, and an ever-expanding knowledge of the regulatory mechanisms underlying T.k. metabolism. Here we review the existing techniques for genetic and biochemical manipulation of T.k. We also introduce a universal platform to generate the first comprehensive deletion and epitope/affinity-tagged archaeal strain libraries. |
| first_indexed | 2024-12-11T05:47:59Z |
| format | Article |
| id | doaj.art-d3819b0b1863464c937e33c4bd86a7d4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T05:47:59Z |
| publishDate | 2012-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d3819b0b1863464c937e33c4bd86a7d42022-12-22T01:18:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2012-06-01310.3389/fmicb.2012.0019526057Genetics techniques for Thermococcus kodakarensisTravis H. Hileman0Thomas James Santangelo1Ohio State UniversityOhio State UniversityThermococcus kodakarensis (T.k.) has emerged as a premier model system for studies of archaeal biochemistry, genetics, and hyperthermophily. This prominence is derived largely from the natural competence of T.k. and the comprehensive, rapid, and facile techniques available for manipulation of the T.k. genome. These genetic capacities are complemented by robust planktonic growth, simple selections and screens, defined in vitro transcription and translation systems, replicative expression plasmids, in vivo reporter constructs, and an ever-expanding knowledge of the regulatory mechanisms underlying T.k. metabolism. Here we review the existing techniques for genetic and biochemical manipulation of T.k. We also introduce a universal platform to generate the first comprehensive deletion and epitope/affinity-tagged archaeal strain libraries.http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00195/fullArchaeaGeneticsThermococcalesThermococcusHyperthermophilerecombination |
| spellingShingle | Travis H. Hileman Thomas James Santangelo Genetics techniques for Thermococcus kodakarensis Frontiers in Microbiology Archaea Genetics Thermococcales Thermococcus Hyperthermophile recombination |
| title | Genetics techniques for Thermococcus kodakarensis |
| title_full | Genetics techniques for Thermococcus kodakarensis |
| title_fullStr | Genetics techniques for Thermococcus kodakarensis |
| title_full_unstemmed | Genetics techniques for Thermococcus kodakarensis |
| title_short | Genetics techniques for Thermococcus kodakarensis |
| title_sort | genetics techniques for thermococcus kodakarensis |
| topic | Archaea Genetics Thermococcales Thermococcus Hyperthermophile recombination |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00195/full |
| work_keys_str_mv | AT travishhileman geneticstechniquesforthermococcuskodakarensis AT thomasjamessantangelo geneticstechniquesforthermococcuskodakarensis |