Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential Oils
<i>Artemisia judaica</i> (ArJ) is a Mediterranean aromatic plant used traditionally to treat gastrointestinal ailments, skin diseases, atherosclerosis, and as an immuno-stimulant. This study describes ArJ essential oil constituents and investigates their wound healing activity. The in vi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/332 |
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| author | Hamdoon A. Mohammed Kamal A. Qureshi Hussein M. Ali Mohsen S. Al-Omar Omar Khan Salman A. A. Mohammed |
| author_facet | Hamdoon A. Mohammed Kamal A. Qureshi Hussein M. Ali Mohsen S. Al-Omar Omar Khan Salman A. A. Mohammed |
| author_sort | Hamdoon A. Mohammed |
| collection | DOAJ |
| description | <i>Artemisia judaica</i> (ArJ) is a Mediterranean aromatic plant used traditionally to treat gastrointestinal ailments, skin diseases, atherosclerosis, and as an immuno-stimulant. This study describes ArJ essential oil constituents and investigates their wound healing activity. The in vitro antioxidant and antibiofilm activities of ArJ essential oil were investigated. The in vivo pro/anti-inflammatory and oxidative/antioxidant markers were compared with standard silver sulfadiazine (SS) in a second-degree skin burn experimental rat model. The gas chromatography-equipped flame ionization detector (GC-FID) analysis of ArJ essential oil revealed the major classes of compounds as oxygenated monoterpenes (>57%) and cinnamic acid derivatives (18.03%). The antimicrobial tests of ArJ essential oil revealed that <i>Bacillus cereus</i>, <i>Candida albicans</i>, and <i>Aspergillus niger</i> were the most susceptible test organisms. Two second-degree burns (each 1 inch square in diameter) were created on the dorsum of rats using an aluminum cylinder heated to 120 °C for 10 s. The wounds were treated either with ArJ or SS ointments for 21 days, while the negative control remained untreated, and biopsies were obtained for histological and biochemical analysis. The ArJ group demonstrated a significant increase in antioxidant superoxide dismutase (SOD) and catalase (CAT) enzymatic activities, while lipid peroxide (LP) levels remained insignificant compared to the negative control group. Additionally, ArJ and SS groups demonstrated a significant decrease in inflammatory levels of tumor necrosis factor α (TNF-α) compared to the negative group, while interleukin 1 beta (IL-1b) and IL-6 were comparable to the negative group. At the same time, anti-inflammatory IL-10 and transforming growth factor beta 1 (TGF-b1) markers increased significantly in the ArJ group compared to the negative control. The ArJ results demonstrated potent wound healing effects, comparable to SS, attributable to antioxidant and anti-inflammatory effects as well as a high proportion of oxygenated monoterpenes and cinnamate derivatives. |
| first_indexed | 2024-03-09T22:44:56Z |
| format | Article |
| id | doaj.art-d3823a7963054ec6bf735783947c05e7 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T22:44:56Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d3823a7963054ec6bf735783947c05e72023-11-23T18:32:10ZengMDPI AGAntioxidants2076-39212022-02-0111233210.3390/antiox11020332Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential OilsHamdoon A. Mohammed0Kamal A. Qureshi1Hussein M. Ali2Mohsen S. Al-Omar3Omar Khan4Salman A. A. Mohammed5Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Pharmaceutics, Unaizah College of Pharmacy, Qassim University, Unaizah 51911, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Qassim University, Buraydah 51452, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraydah 51452, Saudi Arabia<i>Artemisia judaica</i> (ArJ) is a Mediterranean aromatic plant used traditionally to treat gastrointestinal ailments, skin diseases, atherosclerosis, and as an immuno-stimulant. This study describes ArJ essential oil constituents and investigates their wound healing activity. The in vitro antioxidant and antibiofilm activities of ArJ essential oil were investigated. The in vivo pro/anti-inflammatory and oxidative/antioxidant markers were compared with standard silver sulfadiazine (SS) in a second-degree skin burn experimental rat model. The gas chromatography-equipped flame ionization detector (GC-FID) analysis of ArJ essential oil revealed the major classes of compounds as oxygenated monoterpenes (>57%) and cinnamic acid derivatives (18.03%). The antimicrobial tests of ArJ essential oil revealed that <i>Bacillus cereus</i>, <i>Candida albicans</i>, and <i>Aspergillus niger</i> were the most susceptible test organisms. Two second-degree burns (each 1 inch square in diameter) were created on the dorsum of rats using an aluminum cylinder heated to 120 °C for 10 s. The wounds were treated either with ArJ or SS ointments for 21 days, while the negative control remained untreated, and biopsies were obtained for histological and biochemical analysis. The ArJ group demonstrated a significant increase in antioxidant superoxide dismutase (SOD) and catalase (CAT) enzymatic activities, while lipid peroxide (LP) levels remained insignificant compared to the negative control group. Additionally, ArJ and SS groups demonstrated a significant decrease in inflammatory levels of tumor necrosis factor α (TNF-α) compared to the negative group, while interleukin 1 beta (IL-1b) and IL-6 were comparable to the negative group. At the same time, anti-inflammatory IL-10 and transforming growth factor beta 1 (TGF-b1) markers increased significantly in the ArJ group compared to the negative control. The ArJ results demonstrated potent wound healing effects, comparable to SS, attributable to antioxidant and anti-inflammatory effects as well as a high proportion of oxygenated monoterpenes and cinnamate derivatives.https://www.mdpi.com/2076-3921/11/2/332<i>Artemisia judaica</i>essential oilwound healinginflammatory markersantibiofilm activityantioxidant activity |
| spellingShingle | Hamdoon A. Mohammed Kamal A. Qureshi Hussein M. Ali Mohsen S. Al-Omar Omar Khan Salman A. A. Mohammed Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential Oils Antioxidants <i>Artemisia judaica</i> essential oil wound healing inflammatory markers antibiofilm activity antioxidant activity |
| title | Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential Oils |
| title_full | Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential Oils |
| title_fullStr | Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential Oils |
| title_full_unstemmed | Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential Oils |
| title_short | Bio-Evaluation of the Wound Healing Activity of <i>Artemisia judaica</i> L. as Part of the Plant’s Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory, and Antibiofilm Properties of the Plant’s Essential Oils |
| title_sort | bio evaluation of the wound healing activity of i artemisia judaica i l as part of the plant s use in traditional medicine phytochemical antioxidant anti inflammatory and antibiofilm properties of the plant s essential oils |
| topic | <i>Artemisia judaica</i> essential oil wound healing inflammatory markers antibiofilm activity antioxidant activity |
| url | https://www.mdpi.com/2076-3921/11/2/332 |
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