Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice

Epicuticular wax forms the outermost protective barrier of the aerial surfaces of land plants, working in concert with other components of the plant cuticle to prevent uncontrolled loss of water and to provide protection against an array of external environmental stress. In this study, chemically-mu...

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Main Author: Thomas H. Tai
Format: Article
Language:English
Published: Elsevier 2015-07-01
Series:Rice Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1672630815000372
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author Thomas H. Tai
author_facet Thomas H. Tai
author_sort Thomas H. Tai
collection DOAJ
description Epicuticular wax forms the outermost protective barrier of the aerial surfaces of land plants, working in concert with other components of the plant cuticle to prevent uncontrolled loss of water and to provide protection against an array of external environmental stress. In this study, chemically-mutagenized populations of rice (Oryza sativa L.) derived from approximately 4 750 M2 families were screened for adhesion of water droplets resulting in a wet leaf/glossy (wlg) phenotype. Mutants were identified in 11 independently-derived M2 families. Scanning electron microscopy analysis confirmed the association of the wlg phenotype with changes in the epicuticular wax crystals of these plants. The phenotypes of five mutants (7-17A, 26.1, 524.2, 680.2, and 843.1) were confirmed to be the result of single recessive gene mutation. Evaluation of mutants from 3 (6-1A, 7-17A, and 11-39A) of 11 M2 families revealed significant reductions (> 50%) in surface wax content and increases in cuticle membrane permeability.
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spelling doaj.art-d3833bdbec364d549a5b74133472cc7e2022-12-21T19:12:53ZengElsevierRice Science1672-63082015-07-0122417117910.1016/j.rsci.2015.04.002Identification and Characterization of Reduced Epicuticular Wax Mutants in RiceThomas H. TaiEpicuticular wax forms the outermost protective barrier of the aerial surfaces of land plants, working in concert with other components of the plant cuticle to prevent uncontrolled loss of water and to provide protection against an array of external environmental stress. In this study, chemically-mutagenized populations of rice (Oryza sativa L.) derived from approximately 4 750 M2 families were screened for adhesion of water droplets resulting in a wet leaf/glossy (wlg) phenotype. Mutants were identified in 11 independently-derived M2 families. Scanning electron microscopy analysis confirmed the association of the wlg phenotype with changes in the epicuticular wax crystals of these plants. The phenotypes of five mutants (7-17A, 26.1, 524.2, 680.2, and 843.1) were confirmed to be the result of single recessive gene mutation. Evaluation of mutants from 3 (6-1A, 7-17A, and 11-39A) of 11 M2 families revealed significant reductions (> 50%) in surface wax content and increases in cuticle membrane permeability.http://www.sciencedirect.com/science/article/pii/S1672630815000372chemical mutagenesiscuticle waxforward geneticsmembrane permeabilityricescanning electron microscopy
spellingShingle Thomas H. Tai
Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice
Rice Science
chemical mutagenesis
cuticle wax
forward genetics
membrane permeability
rice
scanning electron microscopy
title Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice
title_full Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice
title_fullStr Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice
title_full_unstemmed Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice
title_short Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice
title_sort identification and characterization of reduced epicuticular wax mutants in rice
topic chemical mutagenesis
cuticle wax
forward genetics
membrane permeability
rice
scanning electron microscopy
url http://www.sciencedirect.com/science/article/pii/S1672630815000372
work_keys_str_mv AT thomashtai identificationandcharacterizationofreducedepicuticularwaxmutantsinrice