Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.)
Currently, production of wheat cultivars (Triticum aestivum L.) that show durable field resistance against fungal pathogens is a priority of many breeding programs. This type of resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages. Until now, seven genes (Lr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences
2020-12-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2020.134914 |
| Summary: | Currently, production of wheat cultivars (Triticum aestivum L.) that show durable field
resistance against fungal pathogens is a priority of many breeding programs. This type of
resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages.
Until now, seven genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr75, Lr77 and Lr78)
conferring durable types of resistance against multiple fungal pathogens have been identified
in the wheat gene pool. In this study we showed a multiplex Polymerase Chain Reaction
(multiplex PCR) assay, which was developed for detection of slow rusting resistance
genes Lr34, Lr46, Lr68, using molecular markers: csLV34, Xwmc44 and csGS, respectively.
Identification of molecular markers was performed on 40 selected wheat genotypes which
are the sources of slow rusting genes according to literature reports. Multiplex PCR is an
important tool to reduce the time and cost of analysis. This multiplex PCR protocol can be
applicable for genotyping processes and marker assisted resistance breeding of wheat. |
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| ISSN: | 1899-007X 1899-007X |