Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.)
Currently, production of wheat cultivars (Triticum aestivum L.) that show durable field resistance against fungal pathogens is a priority of many breeding programs. This type of resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages. Until now, seven genes (Lr...
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2020-12-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://doi.org/10.24425/jppr.2020.134914 |
| _version_ | 1828143518453858304 |
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| author | Roksana Skowrońska Agnieszka Tomkowiak Justyna Szwarc Jerzy Nawracała Michał Kwiatek |
| author_facet | Roksana Skowrońska Agnieszka Tomkowiak Justyna Szwarc Jerzy Nawracała Michał Kwiatek |
| author_sort | Roksana Skowrońska |
| collection | DOAJ |
| description | Currently, production of wheat cultivars (Triticum aestivum L.) that show durable field
resistance against fungal pathogens is a priority of many breeding programs. This type of
resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages.
Until now, seven genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr75, Lr77 and Lr78)
conferring durable types of resistance against multiple fungal pathogens have been identified
in the wheat gene pool. In this study we showed a multiplex Polymerase Chain Reaction
(multiplex PCR) assay, which was developed for detection of slow rusting resistance
genes Lr34, Lr46, Lr68, using molecular markers: csLV34, Xwmc44 and csGS, respectively.
Identification of molecular markers was performed on 40 selected wheat genotypes which
are the sources of slow rusting genes according to literature reports. Multiplex PCR is an
important tool to reduce the time and cost of analysis. This multiplex PCR protocol can be
applicable for genotyping processes and marker assisted resistance breeding of wheat. |
| first_indexed | 2024-04-11T19:58:32Z |
| format | Article |
| id | doaj.art-d38477ddeb3e4004bbce31fd05706bae |
| institution | Directory Open Access Journal |
| issn | 1899-007X 1899-007X |
| language | English |
| last_indexed | 2024-04-11T19:58:32Z |
| publishDate | 2020-12-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-d38477ddeb3e4004bbce31fd05706bae2022-12-22T04:05:49ZengPolish Academy of SciencesJournal of Plant Protection Research1899-007X1899-007X2020-12-0160438839810.24425/jppr.2020.134914Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.)Roksana Skowrońska0https://orcid.org/0000-0001-8208-2801Agnieszka Tomkowiak1https://orcid.org/0000-0001-9516-8911Justyna Szwarc2Jerzy Nawracała 3https://orcid.org/0000-0002-6824-156X Michał Kwiatek4https://orcid.org/0000-0001-9442-3124Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, PolandDepartment of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, PolandDepartment of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, PolandDepartment of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, PolandDepartment of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, PolandCurrently, production of wheat cultivars (Triticum aestivum L.) that show durable field resistance against fungal pathogens is a priority of many breeding programs. This type of resistance involves race-nonspecific mechanisms and can be identified at adult-plant stages. Until now, seven genes (Lr34/Yr18, Lr46/Yr29, Lr67/Yr46, Lr68, Lr75, Lr77 and Lr78) conferring durable types of resistance against multiple fungal pathogens have been identified in the wheat gene pool. In this study we showed a multiplex Polymerase Chain Reaction (multiplex PCR) assay, which was developed for detection of slow rusting resistance genes Lr34, Lr46, Lr68, using molecular markers: csLV34, Xwmc44 and csGS, respectively. Identification of molecular markers was performed on 40 selected wheat genotypes which are the sources of slow rusting genes according to literature reports. Multiplex PCR is an important tool to reduce the time and cost of analysis. This multiplex PCR protocol can be applicable for genotyping processes and marker assisted resistance breeding of wheat.https://doi.org/10.24425/jppr.2020.134914leaf rustlr34lr46lr68multiplex pcr |
| spellingShingle | Roksana Skowrońska Agnieszka Tomkowiak Justyna Szwarc Jerzy Nawracała Michał Kwiatek Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.) Journal of Plant Protection Research leaf rust lr34 lr46 lr68 multiplex pcr |
| title | Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.) |
| title_full | Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.) |
| title_fullStr | Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.) |
| title_full_unstemmed | Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.) |
| title_short | Multiplex PCR assay for simultaneous identification of slow rust resistance genes Lr34, Lr46 and Lr68 in wheat (Triticum aestivum L.) |
| title_sort | multiplex pcr assay for simultaneous identification of slow rust resistance genes lr34 lr46 and lr68 in wheat triticum aestivum l |
| topic | leaf rust lr34 lr46 lr68 multiplex pcr |
| url | https://doi.org/10.24425/jppr.2020.134914 |
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