The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice
Abstract In our previous study, enterohepatic 12α-hydroxylated (12α) bile acid (BA) levels were found to be correlated with hepatic triacylglycerol concentration in rats fed high-fat (HF) diet. Since BA composition is diverse depending on animal species, we evaluated whether such a relationship is a...
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Nature Portfolio
2022-10-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-022-20838-9 |
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author | Wakana Iwasaki Ryo Yoshida Hongxia Liu Shota Hori Yuki Otsubo Yasutake Tanaka Masao Sato Satoshi Ishizuka |
author_facet | Wakana Iwasaki Ryo Yoshida Hongxia Liu Shota Hori Yuki Otsubo Yasutake Tanaka Masao Sato Satoshi Ishizuka |
author_sort | Wakana Iwasaki |
collection | DOAJ |
description | Abstract In our previous study, enterohepatic 12α-hydroxylated (12α) bile acid (BA) levels were found to be correlated with hepatic triacylglycerol concentration in rats fed high-fat (HF) diet. Since BA composition is diverse depending on animal species, we evaluated whether such a relationship is applicable in mice in response to an HF diet. C57BL/6JJmsSLC (B6) male mice were fed HF diet for 13 weeks and analyzed for triacylglycerol, cholesterol, oxysterols, and other metabolites in the liver. The BA composition was determined in the liver, small intestinal contents, portal plasma, aortic plasma, and feces. Neutral sterols were also measured in the feces. The ratio of 12α BA/non-12 BA increased in the liver, portal plasma, small intestinal contents, and feces of HF-fed B6 mice. Moreover, a positive correlation was observed between the ratio of fecal 12α BAs/non-12 BAs and hepatic triacylglycerol concentration. The concentration of 7α-hydroxycholesterol was increased in the liver of HF-fed B6 mice, whereas no increase was observed in the hepatic expression of cytochrome P450 family 7 subfamily A member 1. The present study showed that the ratio of 12α BA/non-12 BA in feces is closely associated with hepatic triacylglycerol accumulation in B6 mice fed HF diet. |
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spelling | doaj.art-d38746c08df7423fbee445c3178de4892022-12-22T03:38:23ZengNature PortfolioScientific Reports2045-23222022-10-011211910.1038/s41598-022-20838-9The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J miceWakana Iwasaki0Ryo Yoshida1Hongxia Liu2Shota Hori3Yuki Otsubo4Yasutake Tanaka5Masao Sato6Satoshi Ishizuka7Research Faculty of Agriculture, Hokkaido UniversityResearch Faculty of Agriculture, Hokkaido UniversityResearch Faculty of Agriculture, Hokkaido UniversityResearch Faculty of Agriculture, Hokkaido UniversityFaculty of Agriculture, Kyushu UniversityFaculty of Agriculture, Kyushu UniversityFaculty of Agriculture, Kyushu UniversityResearch Faculty of Agriculture, Hokkaido UniversityAbstract In our previous study, enterohepatic 12α-hydroxylated (12α) bile acid (BA) levels were found to be correlated with hepatic triacylglycerol concentration in rats fed high-fat (HF) diet. Since BA composition is diverse depending on animal species, we evaluated whether such a relationship is applicable in mice in response to an HF diet. C57BL/6JJmsSLC (B6) male mice were fed HF diet for 13 weeks and analyzed for triacylglycerol, cholesterol, oxysterols, and other metabolites in the liver. The BA composition was determined in the liver, small intestinal contents, portal plasma, aortic plasma, and feces. Neutral sterols were also measured in the feces. The ratio of 12α BA/non-12 BA increased in the liver, portal plasma, small intestinal contents, and feces of HF-fed B6 mice. Moreover, a positive correlation was observed between the ratio of fecal 12α BAs/non-12 BAs and hepatic triacylglycerol concentration. The concentration of 7α-hydroxycholesterol was increased in the liver of HF-fed B6 mice, whereas no increase was observed in the hepatic expression of cytochrome P450 family 7 subfamily A member 1. The present study showed that the ratio of 12α BA/non-12 BA in feces is closely associated with hepatic triacylglycerol accumulation in B6 mice fed HF diet.https://doi.org/10.1038/s41598-022-20838-9 |
spellingShingle | Wakana Iwasaki Ryo Yoshida Hongxia Liu Shota Hori Yuki Otsubo Yasutake Tanaka Masao Sato Satoshi Ishizuka The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice Scientific Reports |
title | The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice |
title_full | The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice |
title_fullStr | The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice |
title_full_unstemmed | The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice |
title_short | The ratio of 12α to non-12-hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high-fat diet-fed C57BL/6J mice |
title_sort | ratio of 12α to non 12 hydroxylated bile acids reflects hepatic triacylglycerol accumulation in high fat diet fed c57bl 6j mice |
url | https://doi.org/10.1038/s41598-022-20838-9 |
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